HRPZ_PSESM
ID HRPZ_PSESM Reviewed; 370 AA.
AC Q887C6; Q52474; Q6L901;
DT 26-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 25-MAY-2022, entry version 93.
DE RecName: Full=Harpin HrpZ;
DE AltName: Full=Harpin-Pst;
DE AltName: Full=HrpZ-Pst protein;
GN Name=hrpZ; OrderedLocusNames=PSPTO_1382;
OS Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=223283;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION IN HR.
RC STRAIN=ATCC BAA-871 / DC3000;
RX PubMed=7579616; DOI=10.1094/mpmi-8-0717;
RA Preston G., Huang H.-C., He S.Y., Collmer A.;
RT "The HrpZ proteins of Pseudomonas syringae pvs. syringae, glycinea, and
RT tomato are encoded by an operon containing Yersinia ysc homologs and elicit
RT the hypersensitive response in tomato but not soybean.";
RL Mol. Plant Microbe Interact. 8:717-732(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC BAA-871 / DC3000;
RX PubMed=12580281; DOI=10.1094/mpmi.2003.16.1.43;
RA Fouts D.E., Badel J.L., Ramos A.R., Rapp R.A., Collmer A.;
RT "A Pseudomonas syringae pv. tomato DC3000 Hrp (type III secretion) deletion
RT mutant expressing the Hrp system of bean pathogen P. syringae pv. syringae
RT 61 retains normal host specificity for tomato.";
RL Mol. Plant Microbe Interact. 16:43-52(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ICMP 2844;
RA Inoue Y., Takikawa Y.;
RT "Phylogenic analysis of DNA sequences around the hrpZ regions of
RT Pseudomonas syringae.";
RL Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-871 / DC3000;
RX PubMed=12928499; DOI=10.1073/pnas.1731982100;
RA Buell C.R., Joardar V., Lindeberg M., Selengut J., Paulsen I.T.,
RA Gwinn M.L., Dodson R.J., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R.,
RA Daugherty S.C., Brinkac L.M., Beanan M.J., Haft D.H., Nelson W.C.,
RA Davidsen T.M., Zafar N., Zhou L., Liu J., Yuan Q., Khouri H.M.,
RA Fedorova N.B., Tran B., Russell D., Berry K.J., Utterback T.R.,
RA Van Aken S.E., Feldblyum T.V., D'Ascenzo M., Deng W.-L., Ramos A.R.,
RA Alfano J.R., Cartinhour S., Chatterjee A.K., Delaney T.P., Lazarowitz S.G.,
RA Martin G.B., Schneider D.J., Tang X., Bender C.L., White O., Fraser C.M.,
RA Collmer A.;
RT "The complete genome sequence of the Arabidopsis and tomato pathogen
RT Pseudomonas syringae pv. tomato DC3000.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:10181-10186(2003).
RN [5]
RP SUBCELLULAR LOCATION.
RC STRAIN=ATCC BAA-871 / DC3000;
RX PubMed=11277437; DOI=10.1094/mpmi.2001.14.3.394;
RA Brown I.R., Mansfield J.W., Taira S., Roine E., Romantschuk M.;
RT "Immunocytochemical localization of hrpA and hrpZ supports a role for the
RT hrp pilus in the transfer of effector proteins from Pseudomonas syringae
RT pv. tomato across the host plant cell wall.";
RL Mol. Plant Microbe Interact. 14:394-404(2001).
RN [6]
RP SUBCELLULAR LOCATION.
RC STRAIN=ATCC BAA-871 / DC3000;
RX PubMed=11953310; DOI=10.1093/emboj/21.8.1909;
RA Li C.-M., Brown I.R., Mansfield J.W., Stevens C., Boureau T.,
RA Romantschuk M., Taira S.;
RT "The Hrp pilus of Pseudomonas syringae elongates from its tip and acts as a
RT conduit for translocation of the effector protein HrpZ.";
RL EMBO J. 21:1909-1915(2002).
CC -!- FUNCTION: Harpins are proteins able to elicit hypersensitive response
CC (HR) in non-host plants and are required for pathogenicity in host
CC plants. HrpZ forms ion-conducting pores permeable for cations. Such
CC pore-forming activity may allow nutrient release and/or delivery of
CC virulence factors during bacterial colonization of host plants (By
CC similarity). {ECO:0000250, ECO:0000269|PubMed:7579616}.
CC -!- SUBUNIT: Homomultimeric. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Secreted. Host cell membrane {ECO:0000305}.
CC Note=Secreted via type III secretion system (TTSS), delivered into the
CC host intercellular space. HrpZ travels through the hrp pilus and it is
CC secreted only from the pilus tip.
CC -!- DOMAIN: The glycine-rich region is characteristic of harpins.
CC -!- SIMILARITY: Belongs to the harpin HrpZ family. {ECO:0000305}.
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DR EMBL; AF232004; AAB00127.1; -; Genomic_DNA.
DR EMBL; AB112567; BAD20880.1; -; Genomic_DNA.
DR EMBL; AE016853; AAO54904.1; -; Genomic_DNA.
DR RefSeq; NP_791209.1; NC_004578.1.
DR RefSeq; WP_005763902.1; NC_004578.1.
DR AlphaFoldDB; Q887C6; -.
DR STRING; 223283.PSPTO_1382; -.
DR DNASU; 1183018; -.
DR EnsemblBacteria; AAO54904; AAO54904; PSPTO_1382.
DR GeneID; 1183018; -.
DR KEGG; pst:PSPTO_1382; -.
DR PATRIC; fig|223283.9.peg.1404; -.
DR eggNOG; ENOG5032BF5; Bacteria.
DR HOGENOM; CLU_071571_0_0_6; -.
DR OMA; MDDNPAQ; -.
DR OrthoDB; 1412217at2; -.
DR Proteomes; UP000002515; Chromosome.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0020002; C:host cell plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0006811; P:ion transport; IEA:UniProtKB-KW.
DR GO; GO:0034053; P:modulation by symbiont of host defense-related programmed cell death; IEA:UniProtKB-KW.
DR InterPro; IPR006961; HrpN/Z.
DR Pfam; PF04877; Harpin; 1.
PE 1: Evidence at protein level;
KW Host cell membrane; Host membrane; Hypersensitive response elicitation;
KW Ion channel; Ion transport; Membrane; Reference proteome; Repeat; Secreted;
KW Transport; Virulence.
FT CHAIN 1..370
FT /note="Harpin HrpZ"
FT /id="PRO_0000220301"
FT REPEAT 235..240
FT /note="1-1"
FT REPEAT 286..291
FT /note="1-2"
FT REGION 221..257
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 235..291
FT /note="2 X 7 AA repeats of G-G-G-L-G-[ST]-P"
FT REGION 282..314
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VARIANT 265
FT /note="V -> A (in strain: ICMP 2844)"
FT CONFLICT 304
FT /note="A -> V (in Ref. 1 and 2)"
FT /evidence="ECO:0000305"
FT CONFLICT 324
FT /note="L -> R (in Ref. 1 and 2)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 370 AA; 36513 MW; 54E3FC775AD20938 CRC64;
MQALNSISSL QTSASLFPVS LNSDVSANTS TSSKELKAVI DQLVQALTQS GQLDETSPLG
KMLAKAMAAD GKSANSIDDI TASLDKLIHE KLGDNFGASA GIGAGGGGGG IGGAGSGSGV
GGGLSSDAGA GQSDLMSQVL NGLGKAVLDD LLTPSGEGGT TFSSDDMPTL EKVAQFMDDN
KAQFPTRDGG SWMNELKEDN GLDAQETAQF RSALDVIGQQ LGQQQGDASG VTSGGGLGSP
VSDSSLGNPA IDANTGPAAN GNASVDVGQL IGQLIDRGLQ SVSSGGGLGT PVDNSTQPTG
GTPAANPTGN VSNQDLGQLL SGLLQRGLEA TLQDAGNTGA DLQSSAAQVA AQLINALLQG
TNNQTNQAVA
