ID   HRPZ_PSESY              Reviewed;         341 AA.
AC   P35674; O31180;
DT   01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1994, sequence version 1.
DT   25-MAY-2022, entry version 89.
DE   RecName: Full=Harpin HrpZ;
DE   AltName: Full=Harpin-Pss;
DE   AltName: Full=HrpZ-Pss protein;
GN   Name=hrpZ;
OS   Pseudomonas syringae pv. syringae.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas; Pseudomonas syringae.
OX   NCBI_TaxID=321;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-7, AND FUNCTION IN
RP   HR.
RC   STRAIN=Pss61;
RX   PubMed=8324821; DOI=10.1016/0092-8674(93)90354-s;
RA   He S.Y., Huang H.-C., Collmer A.;
RT   "Pseudomonas syringae pv. syringae harpinPss: a protein that is secreted
RT   via the Hrp pathway and elicits the hypersensitive response in plants.";
RL   Cell 73:1255-1266(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=NV;
RA   Appel M., Hampf M., Mansvelt E.L., Hapgood J.P., Bellstedt D.U.;
RT   "Amplification, sequence and expression of the hrpZ gene of the bacterial
RT   canker pathogen, Pseudomonas syringae pv. syringae strain NV.";
RL   Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   TRANSCRIPTIONAL REGULATION.
RC   STRAIN=Pss61;
RX   PubMed=8106313; DOI=10.1128/jb.176.4.1025-1036.1994;
RA   Xiao Y., Heu S., Yi J., Lu Y., Hutcheson S.W.;
RT   "Identification of a putative alternate sigma factor and characterization
RT   of a multicomponent regulatory cascade controlling the expression of
RT   Pseudomonas syringae pv. syringae Pss61 hrp and hrmA genes.";
RL   J. Bacteriol. 176:1025-1036(1994).
RN   [4]
RP   FUNCTION IN HR.
RC   STRAIN=Pss61;
RX   PubMed=7579616; DOI=10.1094/mpmi-8-0717;
RA   Preston G., Huang H.-C., He S.Y., Collmer A.;
RT   "The HrpZ proteins of Pseudomonas syringae pvs. syringae, glycinea, and
RT   tomato are encoded by an operon containing Yersinia ysc homologs and elicit
RT   the hypersensitive response in tomato but not soybean.";
RL   Mol. Plant Microbe Interact. 8:717-732(1995).
RN   [5]
RP   SUBCELLULAR LOCATION.
RC   STRAIN=Pss61;
RX   PubMed=7579617; DOI=10.1094/mpmi-8-0733;
RA   Huang H.-C., Lin R.-H., Chang C.-J., Collmer A., Deng W.-L.;
RT   "The complete hrp gene cluster of Pseudomonas syringae pv. syringae 61
RT   includes two blocks of genes required for harpinPss secretion that are
RT   arranged colinearly with Yersinia ysc homologs.";
RL   Mol. Plant Microbe Interact. 8:733-746(1995).
RN   [6]
RP   SUBCELLULAR LOCATION.
RC   STRAIN=Pss61;
RA   Hoyos M.E., Stanley C.M., He S.Y., Pike S., Pu X.-A., Novacky A.;
RT   "The interaction of harpinPss, with plant cell walls.";
RL   Mol. Plant Microbe Interact. 9:608-616(1996).
RN   [7]
RP   SUBUNIT.
RC   STRAIN=Pss61;
RX   AGRICOLA=IND21807826; DOI=10.1006/pmpp.1997.0120;
RA   Chen C.-H., Lin H.-J., Feng T.-Y.;
RT   "An amphipathic protein from sweet pepper can dissociate harpinPss
RT   multimeric forms and intensify the harpinPss-mediated hypersensitive
RT   response.";
RL   Physiol. Mol. Plant Pathol. 52:139-149(1998).
CC   -!- FUNCTION: Harpins are proteins able to elicit hypersensitive response
CC       (HR) in non-host plants and are required for pathogenicity in host
CC       plants. HrpZ forms ion-conducting pores permeable for cations. Such
CC       pore-forming activity may allow nutrient release and/or delivery of
CC       virulence factors during bacterial colonization of host plants (By
CC       similarity). {ECO:0000250, ECO:0000269|PubMed:7579616,
CC       ECO:0000269|PubMed:8324821}.
CC   -!- SUBUNIT: Homomultimeric. {ECO:0000269|Ref.7}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}. Host cell membrane
CC       {ECO:0000250}. Note=Secreted via type III secretion system (TTSS),
CC       delivered into the host intercellular space. HrpZ travels through the
CC       hrp pilus and it is secreted only from the pilus tip (By similarity).
CC       {ECO:0000250}.
CC   -!- INDUCTION: Regulated by HrpRS and HrpL. {ECO:0000269|PubMed:8106313}.
CC   -!- DOMAIN: The glycine-rich region is characteristic of harpins.
CC   -!- SIMILARITY: Belongs to the harpin HrpZ family. {ECO:0000305}.
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DR   EMBL; L14775; AAA25839.1; -; Genomic_DNA.
DR   EMBL; AF031667; AAB86735.1; -; Genomic_DNA.
DR   PIR; A40706; A40706.
DR   RefSeq; WP_003433477.1; NZ_MLEV01000001.1.
DR   AlphaFoldDB; P35674; -.
DR   TCDB; 1.C.56.1.1; the pseudomonas syringae hrpz target host cell membrane cation channel (hrpz) family.
DR   PRIDE; P35674; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0020002; C:host cell plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   GO; GO:0006811; P:ion transport; IEA:UniProtKB-KW.
DR   GO; GO:0034053; P:modulation by symbiont of host defense-related programmed cell death; IEA:UniProtKB-KW.
DR   InterPro; IPR006961; HrpN/Z.
DR   Pfam; PF04877; Harpin; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Host cell membrane; Host membrane;
KW   Hypersensitive response elicitation; Ion channel; Ion transport; Membrane;
KW   Repeat; Secreted; Transport; Virulence.
FT   CHAIN           1..341
FT                   /note="Harpin HrpZ"
FT                   /id="PRO_0000220302"
FT   REPEAT          210..216
FT                   /note="1-1"
FT   REPEAT          265..271
FT                   /note="1-2"
FT   REPEAT          276..279
FT                   /note="2-1"
FT   REPEAT          311..314
FT                   /note="2-2"
FT   REGION          199..228
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          210..271
FT                   /note="2 X 7 AA repeats of G-G-G-L-G-T-P"
FT   REGION          276..314
FT                   /note="2 X 4 AA repeats of Q-T-G-T"
FT   VARIANT         25
FT                   /note="A -> T (in strain: NV)"
FT   VARIANT         30..31
FT                   /note="ST -> ASS (in strain: NV)"
FT   VARIANT         102
FT                   /note="S -> N (in strain: NV)"
FT   VARIANT         120
FT                   /note="A -> V (in strain: NV)"
FT   VARIANT         202
FT                   /note="D -> G (in strain: NV)"
FT   VARIANT         205
FT                   /note="S -> G (in strain: NV)"
FT   VARIANT         221
FT                   /note="N -> S (in strain: NV)"
FT   VARIANT         225..226
FT                   /note="VM -> GVK (in strain: NV)"
FT   VARIANT         242..244
FT                   /note="NTR -> TTS (in strain: NV)"
FT   VARIANT         280
FT                   /note="S -> A (in strain: NV)"
FT   VARIANT         313
FT                   /note="G -> A (in strain: NV)"
SQ   SEQUENCE   341 AA;  34721 MW;  75FB7329B5380179 CRC64;
     MQSLSLNSSS LQTPAMALVL VRPEAETTGS TSSKALQEVV VKLAEELMRN GQLDDSSPLG
     KLLAKSMAAD GKAGGGIEDV IAALDKLIHE KLGDNFGASA DSASGTGQQD LMTQVLNGLA
     KSMLDDLLTK QDGGTSFSED DMPMLNKIAQ FMDDNPAQFP KPDSGSWVNE LKEDNFLDGD
     ETAAFRSALD IIGQQLGNQQ SDAGSLAGTG GGLGTPSSFS NNSSVMGDPL IDANTGPGDS
     GNTRGEAGQL IGELIDRGLQ SVLAGGGLGT PVNTPQTGTS ANGGQSAQDL DQLLGGLLLK
     GLEATLKDAG QTGTDVQSSA AQIATLLVST LLQGTRNQAA A