HRS1_ARATH
ID HRS1_ARATH Reviewed; 344 AA.
AC Q9FX67;
DT 12-APR-2017, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 162.
DE RecName: Full=Transcription factor HRS1 {ECO:0000305};
DE AltName: Full=MYB-domain transcription factor HRS1 {ECO:0000305};
DE AltName: Full=NIGT1 protein homolog {ECO:0000303|PubMed:25723764};
DE Short=AtNIGT1 {ECO:0000303|PubMed:25723764};
DE AltName: Full=Protein HYPERSENSITIVITY TO LOW PI-ELICITED PRIMARY ROOT SHORTENING 1 {ECO:0000303|PubMed:19341407};
GN Name=HRS1 {ECO:0000303|PubMed:19341407};
GN Synonyms=NIGT1 {ECO:0000303|PubMed:25723764};
GN OrderedLocusNames=At1g13300 {ECO:0000312|Araport:AT1G13300};
GN ORFNames=T6J4.6 {ECO:0000312|EMBL:AAG09552.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP FUNCTION, TISSUE SPECIFICITY, INDUCTION BY PHOSPHATE DEPRIVATION, AND
RP DISRUPTION PHENOTYPE.
RX PubMed=19341407; DOI=10.1111/j.1744-7909.2009.00819.x;
RA Liu H., Yang H., Wu C., Feng J., Liu X., Qin H., Wang D.;
RT "Overexpressing HRS1 confers hypersensitivity to low phosphate-elicited
RT inhibition of primary root growth in Arabidopsis thaliana.";
RL J. Integr. Plant Biol. 51:382-392(2009).
RN [5]
RP FUNCTION.
RX PubMed=22545134; DOI=10.1371/journal.pone.0035764;
RA Wu C., Feng J., Wang R., Liu H., Yang H., Rodriguez P.L., Qin H., Liu X.,
RA Wang D.;
RT "HRS1 acts as a negative regulator of abscisic acid signaling to promote
RT timely germination of Arabidopsis seeds.";
RL PLoS ONE 7:E35764-E35764(2012).
RN [6]
RP FUNCTION, SUBCELLULAR LOCATION, AND INDUCTION BY NITRATE.
RX PubMed=25723764; DOI=10.1038/ncomms7274;
RA Medici A., Marshall-Colon A., Ronzier E., Szponarski W., Wang R., Gojon A.,
RA Crawford N.M., Ruffel S., Coruzzi G.M., Krouk G.;
RT "AtNIGT1/HRS1 integrates nitrate and phosphate signals at the Arabidopsis
RT root tip.";
RL Nat. Commun. 6:6274-6274(2015).
RN [7]
RP INDUCTION.
RX PubMed=27419465; DOI=10.1111/tpj.13272;
RA Menz J., Li Z., Schulze W.X., Ludewig U.;
RT "Early nitrogen-deprivation responses in Arabidopsis roots reveal distinct
RT differences on transcriptome and (phospho-) proteome levels between nitrate
RT and ammonium nutrition.";
RL Plant J. 88:717-734(2016).
CC -!- FUNCTION: Transcription factor involved in nitrate and phosphate
CC signaling in roots. Integrates nitrate and phosphate starvation
CC responses and adaptation of root architecture depending on nutrient
CC availabilities. Acts downstream of the nitrate sensor and transporter
CC NPF6.3/NRT1.1. Represses primary root development in response to
CC phosphate deficiency conditions, only when nitrate is present
CC (PubMed:25723764). Involved in the modulation of primary root and root
CC hair growth in phosphate-deprived environement (PubMed:19341407). May
CC be required for suppressing abscisic acid (ABA) signaling in
CC germinating embryo axis, which promotes the timely germination of seeds
CC (PubMed:22545134). {ECO:0000269|PubMed:19341407,
CC ECO:0000269|PubMed:22545134, ECO:0000269|PubMed:25723764}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00625,
CC ECO:0000269|PubMed:25723764}.
CC -!- TISSUE SPECIFICITY: Expressed in the root hair region and root hair
CC cells. {ECO:0000269|PubMed:19341407}.
CC -!- INDUCTION: Induced by nitrate (PubMed:25723764). Down-regulated under
CC nitrate deprivation conditions (PubMed:27419465).
CC {ECO:0000269|PubMed:25723764, ECO:0000269|PubMed:27419465}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype under normal growth
CC conditions. {ECO:0000269|PubMed:19341407}.
CC -!- MISCELLANEOUS: Plants over-expressing display increased susceptibility
CC in primary root shortening under low phosphate conditions.
CC {ECO:0000269|PubMed:19341407}.
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DR EMBL; AC011810; AAG09552.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE28996.1; -; Genomic_DNA.
DR EMBL; AF367336; AAK32923.1; -; mRNA.
DR EMBL; AY124865; AAM70574.1; -; mRNA.
DR PIR; F86267; F86267.
DR RefSeq; NP_563926.1; NM_101201.2.
DR AlphaFoldDB; Q9FX67; -.
DR SMR; Q9FX67; -.
DR IntAct; Q9FX67; 1.
DR STRING; 3702.AT1G13300.1; -.
DR PaxDb; Q9FX67; -.
DR PRIDE; Q9FX67; -.
DR ProteomicsDB; 230142; -.
DR EnsemblPlants; AT1G13300.1; AT1G13300.1; AT1G13300.
DR GeneID; 837890; -.
DR Gramene; AT1G13300.1; AT1G13300.1; AT1G13300.
DR KEGG; ath:AT1G13300; -.
DR Araport; AT1G13300; -.
DR TAIR; locus:2205339; AT1G13300.
DR eggNOG; ENOG502QSXV; Eukaryota.
DR HOGENOM; CLU_036551_0_0_1; -.
DR InParanoid; Q9FX67; -.
DR OMA; ESMRKDP; -.
DR OrthoDB; 907731at2759; -.
DR PhylomeDB; Q9FX67; -.
DR PRO; PR:Q9FX67; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9FX67; baseline and differential.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:TAIR.
DR GO; GO:0001217; F:DNA-binding transcription repressor activity; IDA:TAIR.
DR GO; GO:0071456; P:cellular response to hypoxia; HEP:TAIR.
DR GO; GO:1901699; P:cellular response to nitrogen compound; IEP:TAIR.
DR GO; GO:0016036; P:cellular response to phosphate starvation; IMP:TAIR.
DR GO; GO:0009788; P:negative regulation of abscisic acid-activated signaling pathway; IMP:CACAO.
DR GO; GO:0080022; P:primary root development; IMP:TAIR.
DR GO; GO:0032107; P:regulation of response to nutrient levels; IGI:TAIR.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; TAS:TAIR.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR044787; HRS1-like.
DR InterPro; IPR017930; Myb_dom.
DR InterPro; IPR006447; Myb_dom_plants.
DR PANTHER; PTHR31003; PTHR31003; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR TIGRFAMs; TIGR01557; myb_SHAQKYF; 1.
DR PROSITE; PS51294; HTH_MYB; 1.
PE 2: Evidence at transcript level;
KW DNA-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..344
FT /note="Transcription factor HRS1"
FT /id="PRO_0000439543"
FT DOMAIN 178..238
FT /note="HTH myb-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DNA_BIND 209..234
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT REGION 88..184
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 269..322
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 108..123
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 137..181
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 269..290
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 344 AA; 39057 MW; 2D5E8861A0CE4366 CRC64;
MIKKFSNMDY NQKRERCGQY IEALEEERRK IHVFQRELPL CLDLVTQAIE ACKRELPEMT
TENMYGQPEC SEQTTGECGP VLEQFLTIKD SSTSNEEEDE EFDDEHGNHD PDNDSEDKNT
KSDWLKSVQL WNQPDHPLLP KEERLQQETM TRDESMRKDP MVNGGEGRKR EAEKDGGGGR
KQRRCWSSQL HRRFLNALQH LGGPHVATPK QIREFMKVDG LTNDEVKSHL QKYRLHTRRP
RQTVPNNGNS QTQHFVVVGG LWVPQSDYST GKTTGGATTS STTTTTGIYG TMAAPPPPQW
PSHSNYRPSI IVDEGSGSHS EGVVVRCSSP AMSSSTRNHY VKNN
