ID   HRT2_HEVBR              Reviewed;         284 AA.
AC   Q8W3U4;
DT   05-SEP-2012, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2002, sequence version 1.
DT   03-AUG-2022, entry version 74.
DE   RecName: Full=Rubber cis-polyprenyltransferase HRT2;
DE            EC=2.5.1.20;
GN   Name=HRT2;
OS   Hevea brasiliensis (Para rubber tree) (Siphonia brasiliensis).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Malpighiales; Euphorbiaceae; Crotonoideae; Micrandreae;
OC   Hevea.
OX   NCBI_TaxID=3981;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, AND TISSUE
RP   SPECIFICITY.
RX   PubMed=14622254; DOI=10.1046/j.1432-1033.2003.03863.x;
RA   Asawatreratanakul K., Zhang Y.W., Wititsuwannakul D., Wititsuwannakul R.,
RA   Takahashi S., Rattanapittayaporn A., Koyama T.;
RT   "Molecular cloning, expression and characterization of cDNA encoding cis-
RT   prenyltransferases from Hevea brasiliensis. A key factor participating in
RT   natural rubber biosynthesis.";
RL   Eur. J. Biochem. 270:4671-4680(2003).
CC   -!- FUNCTION: Proposed to be involved in rubber biosynthesis as a particle-
CC       bound rubber transferase responsible for the cis-1,4-polymerization of
CC       isoprene subunits. Probably requires additional factors for the
CC       production of high molecular mass rubber.
CC       {ECO:0000269|PubMed:14622254}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(cis-prenyl)n-diphosphate + isopentenyl diphosphate = (cis-
CC         prenyl)(n+1)-diphosphate + diphosphate; Xref=Rhea:RHEA:18801,
CC         Rhea:RHEA-COMP:10564, Rhea:RHEA-COMP:14484, ChEBI:CHEBI:33019,
CC         ChEBI:CHEBI:83374, ChEBI:CHEBI:128769; EC=2.5.1.20;
CC         Evidence={ECO:0000269|PubMed:14622254};
CC   -!- TISSUE SPECIFICITY: Predominnatly expressed in latex.
CC       {ECO:0000269|PubMed:14622254}.
CC   -!- SIMILARITY: Belongs to the UPP synthase family. {ECO:0000305}.
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DR   EMBL; AB064661; BAB83522.1; -; mRNA.
DR   AlphaFoldDB; Q8W3U4; -.
DR   SMR; Q8W3U4; -.
DR   BioCyc; MetaCyc:MON-20090; -.
DR   BRENDA; 2.5.1.20; 2665.
DR   GO; GO:0050267; F:rubber cis-polyprenylcistransferase activity; IEA:UniProtKB-EC.
DR   CDD; cd00475; Cis_IPPS; 1.
DR   Gene3D; 3.40.1180.10; -; 1.
DR   InterPro; IPR001441; UPP_synth-like.
DR   InterPro; IPR018520; UPP_synth-like_CS.
DR   InterPro; IPR036424; UPP_synth-like_sf.
DR   PANTHER; PTHR10291; PTHR10291; 1.
DR   Pfam; PF01255; Prenyltransf; 1.
DR   SUPFAM; SSF64005; SSF64005; 1.
DR   TIGRFAMs; TIGR00055; uppS; 1.
DR   PROSITE; PS01066; UPP_SYNTHASE; 1.
PE   1: Evidence at protein level;
KW   Transferase.
FT   CHAIN           1..284
FT                   /note="Rubber cis-polyprenyltransferase HRT2"
FT                   /id="PRO_0000418868"
FT   ACT_SITE        41
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   284 AA;  32683 MW;  204E3B95B702EED9 CRC64;
     MELYNGERPS VFRLLGKYMR KGLYSILTQG PIPTHIAFIL DGNGRFAKKH KLPEGGGHKA
     GFLALLNVLT YCYELGVKYA TIYAFSIDNF RRKPHEVQYV MNLMLEKIEG MIMEESIINA
     YDICVRFVGN LKLLDEPLKT AADKIMRATA KNSKFVLLLA VCYTSTDEIV HAVEESSKDK
     LKSDEICNDG NGDCVIKIEE MEPYSEIKLV ELERNTYINP YPDVLIRTSG ETRLSNYLLW
     QTTNCILYSP HALWPEIGLR HVVWAVINCQ RHYSYLEKHK EYLK