HS12B_HUMAN
ID HS12B_HUMAN Reviewed; 686 AA.
AC Q96MM6; D3DVX7; Q2TAK3; Q9BR52;
DT 10-JAN-2003, integrated into UniProtKB/Swiss-Prot.
DT 10-JAN-2003, sequence version 2.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=Heat shock 70 kDa protein 12B;
GN Name=HSPA12B; Synonyms=C20orf60;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Placenta;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=11780052; DOI=10.1038/414865a;
RA Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R.,
RA Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L.,
RA Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P.,
RA Bird C.P., Blakey S.E., Bridgeman A.M., Brown A.J., Buck D., Burrill W.D.,
RA Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G.,
RA Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E.,
RA Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D.,
RA Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P.,
RA Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E.,
RA Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J.,
RA Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D.,
RA Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S.,
RA Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D.,
RA Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A.,
RA Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T.,
RA Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I.,
RA Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M.,
RA Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D.,
RA Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M.,
RA Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A.,
RA Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L.,
RA Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L.,
RA Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J.;
RT "The DNA sequence and comparative analysis of human chromosome 20.";
RL Nature 414:865-871(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain, and Ovary;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP IDENTIFICATION, AND TISSUE SPECIFICITY.
RX PubMed=12552099; DOI=10.1073/pnas.252764399;
RA Han Z., Truong Q.A., Park S., Breslow J.L.;
RT "Two Hsp70 family members expressed in atherosclerotic lesions.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:1256-1261(2003).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-25; SER-29 AND SER-276, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=24275569; DOI=10.1016/j.jprot.2013.11.014;
RA Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L.,
RA Ye M., Zou H.;
RT "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver
RT phosphoproteome.";
RL J. Proteomics 96:253-262(2014).
CC -!- INTERACTION:
CC Q96MM6; A8MQ03: CYSRT1; NbExp=3; IntAct=EBI-10291310, EBI-3867333;
CC Q96MM6; O95967: EFEMP2; NbExp=3; IntAct=EBI-10291310, EBI-743414;
CC Q96MM6; O43301: HSPA12A; NbExp=3; IntAct=EBI-10291310, EBI-296980;
CC Q96MM6; Q15323: KRT31; NbExp=6; IntAct=EBI-10291310, EBI-948001;
CC Q96MM6; O76011: KRT34; NbExp=3; IntAct=EBI-10291310, EBI-1047093;
CC Q96MM6; Q6A162: KRT40; NbExp=3; IntAct=EBI-10291310, EBI-10171697;
CC Q96MM6; P60410: KRTAP10-8; NbExp=3; IntAct=EBI-10291310, EBI-10171774;
CC Q96MM6; Q7Z3S9: NOTCH2NLA; NbExp=3; IntAct=EBI-10291310, EBI-945833;
CC Q96MM6; P0DPK4: NOTCH2NLC; NbExp=3; IntAct=EBI-10291310, EBI-22310682;
CC Q96MM6; Q12933: TRAF2; NbExp=3; IntAct=EBI-10291310, EBI-355744;
CC -!- TISSUE SPECIFICITY: Highest expression in muscle and heart. Lower
CC levels in liver and kidney. {ECO:0000269|PubMed:12552099}.
CC -!- SIMILARITY: Belongs to the heat shock protein 70 family. {ECO:0000305}.
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DR EMBL; AK056712; BAB71261.1; -; mRNA.
DR EMBL; AL109804; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471133; EAX10516.1; -; Genomic_DNA.
DR EMBL; BC110881; AAI10882.1; -; mRNA.
DR EMBL; BC117284; AAI17285.1; -; mRNA.
DR EMBL; BC126263; AAI26264.1; -; mRNA.
DR CCDS; CCDS13061.1; -.
DR RefSeq; NP_001184256.1; NM_001197327.1.
DR RefSeq; NP_443202.3; NM_052970.4.
DR RefSeq; XP_016883121.1; XM_017027632.1.
DR AlphaFoldDB; Q96MM6; -.
DR BioGRID; 125532; 72.
DR IntAct; Q96MM6; 11.
DR STRING; 9606.ENSP00000254963; -.
DR iPTMnet; Q96MM6; -.
DR PhosphoSitePlus; Q96MM6; -.
DR BioMuta; HSPA12B; -.
DR DMDM; 27734244; -.
DR EPD; Q96MM6; -.
DR jPOST; Q96MM6; -.
DR MassIVE; Q96MM6; -.
DR PaxDb; Q96MM6; -.
DR PeptideAtlas; Q96MM6; -.
DR PRIDE; Q96MM6; -.
DR ProteomicsDB; 77374; -.
DR Antibodypedia; 2863; 129 antibodies from 24 providers.
DR DNASU; 116835; -.
DR Ensembl; ENST00000254963.7; ENSP00000254963.2; ENSG00000132622.11.
DR GeneID; 116835; -.
DR KEGG; hsa:116835; -.
DR MANE-Select; ENST00000254963.7; ENSP00000254963.2; NM_052970.5; NP_443202.3.
DR UCSC; uc002wjd.4; human.
DR CTD; 116835; -.
DR DisGeNET; 116835; -.
DR GeneCards; HSPA12B; -.
DR HGNC; HGNC:16193; HSPA12B.
DR HPA; ENSG00000132622; Low tissue specificity.
DR MIM; 610702; gene.
DR neXtProt; NX_Q96MM6; -.
DR OpenTargets; ENSG00000132622; -.
DR PharmGKB; PA25771; -.
DR VEuPathDB; HostDB:ENSG00000132622; -.
DR eggNOG; KOG0101; Eukaryota.
DR GeneTree; ENSGT00940000159032; -.
DR InParanoid; Q96MM6; -.
DR OMA; IYKHTME; -.
DR OrthoDB; 804087at2759; -.
DR PhylomeDB; Q96MM6; -.
DR TreeFam; TF329492; -.
DR PathwayCommons; Q96MM6; -.
DR Reactome; R-HSA-3371453; Regulation of HSF1-mediated heat shock response.
DR SignaLink; Q96MM6; -.
DR BioGRID-ORCS; 116835; 10 hits in 1068 CRISPR screens.
DR ChiTaRS; HSPA12B; human.
DR GenomeRNAi; 116835; -.
DR Pharos; Q96MM6; Tbio.
DR PRO; PR:Q96MM6; -.
DR Proteomes; UP000005640; Chromosome 20.
DR RNAct; Q96MM6; protein.
DR Bgee; ENSG00000132622; Expressed in tendon of biceps brachii and 165 other tissues.
DR ExpressionAtlas; Q96MM6; baseline and differential.
DR Genevisible; Q96MM6; HS.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR InterPro; IPR043129; ATPase_NBD.
DR InterPro; IPR029509; HSPA12B.
DR PANTHER; PTHR14187:SF39; PTHR14187:SF39; 1.
DR SUPFAM; SSF53067; SSF53067; 2.
PE 1: Evidence at protein level;
KW ATP-binding; Nucleotide-binding; Phosphoprotein; Reference proteome.
FT CHAIN 1..686
FT /note="Heat shock 70 kDa protein 12B"
FT /id="PRO_0000078294"
FT REGION 12..53
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 30..53
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 25
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:24275569"
FT MOD_RES 29
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:24275569"
FT MOD_RES 42
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q9CZJ2"
FT MOD_RES 44
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9CZJ2"
FT MOD_RES 46
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9CZJ2"
FT MOD_RES 276
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:24275569"
FT VARIANT 23
FT /note="V -> L (in dbSNP:rs34414870)"
FT /id="VAR_049621"
FT VARIANT 270
FT /note="R -> H (in dbSNP:rs6139194)"
FT /id="VAR_059362"
FT CONFLICT 300
FT /note="E -> D (in Ref. 1; BAB71261)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 686 AA; 75688 MW; 5F873AE97E366606 CRC64;
MLAVPEMGLQ GLYIGSSPER SPVPSPPGSP RTQESCGIAP LTPSQSPKPE VRAPQQASFS
VVVAIDFGTT SSGYAFSFAS DPEAIHMMRK WEGGDPGVAH QKTPTCLLLT PEGAFHSFGY
TARDYYHDLD PEEARDWLYF EKFKMKIHSA TDLTLKTQLE AVNGKTMPAL EVFAHALRFF
REHALQELRE QSPSLPEKDT VRWVLTVPAI WKQPAKQFMR EAAYLAGLVS RENAEQLLIA
LEPEAASVYC RKLRLHQLLD LSGRAPGGGR LGERRSIDSS FRQAREQLRR SRHSRTFLVE
SGVGELWAEM QAGDRYVVAD CGGGTVDLTV HQLEQPHGTL KELYKASGGP YGAVGVDLAF
EQLLCRIFGE DFIATFKRQR PAAWVDLTIA FEARKRTAGP HRAGALNISL PFSFIDFYRK
QRGHNVETAL RRSSVNFVKW SSQGMLRMSC EAMNELFQPT VSGIIQHIEA LLARPEVQGV
KLLFLVGGFA ESAVLQHAVQ AALGARGLRV VVPHDVGLTI LKGAVLFGQA PGVVRVRRSP
LTYGVGVLNR FVPGRHPPEK LLVRDGRRWC TDVFERFVAA EQSVALGEEV RRSYCPARPG
QRRVLINLYC CAAEDARFIT DPGVRKCGAL SLELEPADCG QDTAGAPPGR REIRAAMQFG
DTEIKVTAVD VSTNRSVRAS IDFLSN
