HS12B_MOUSE
ID HS12B_MOUSE Reviewed; 685 AA.
AC Q9CZJ2;
DT 10-JAN-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 123.
DE RecName: Full=Heat shock 70 kDa protein 12B;
GN Name=Hspa12b;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Embryo;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP PROTEIN SEQUENCE OF 577-591, AND IDENTIFICATION BY MASS SPECTROMETRY.
RC STRAIN=OF1; TISSUE=Hippocampus;
RA Lubec G., Sunyer B., Chen W.-Q.;
RL Submitted (JAN-2009) to UniProtKB.
RN [4]
RP IDENTIFICATION, AND TISSUE SPECIFICITY.
RX PubMed=12552099; DOI=10.1073/pnas.252764399;
RA Han Z., Truong Q.A., Park S., Breslow J.L.;
RT "Two Hsp70 family members expressed in atherosclerotic lesions.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:1256-1261(2003).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-42, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=17242355; DOI=10.1073/pnas.0609836104;
RA Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.;
RT "Large-scale phosphorylation analysis of mouse liver.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-42; SER-44 AND SER-46, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas,
RC Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- TISSUE SPECIFICITY: Expressed most strongly in heart with little or no
CC expression in other tissues. In the aorta, preferentially expressed in
CC lesions. {ECO:0000269|PubMed:12552099}.
CC -!- SIMILARITY: Belongs to the heat shock protein 70 family. {ECO:0000305}.
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DR EMBL; AK012548; BAB28308.1; -; mRNA.
DR EMBL; BC011103; AAH11103.1; -; mRNA.
DR EMBL; AY196790; AAO37639.1; -; mRNA.
DR CCDS; CCDS16755.1; -.
DR RefSeq; NP_082582.1; NM_028306.3.
DR AlphaFoldDB; Q9CZJ2; -.
DR SMR; Q9CZJ2; -.
DR BioGRID; 215479; 2.
DR IntAct; Q9CZJ2; 1.
DR STRING; 10090.ENSMUSP00000096950; -.
DR iPTMnet; Q9CZJ2; -.
DR PhosphoSitePlus; Q9CZJ2; -.
DR SwissPalm; Q9CZJ2; -.
DR jPOST; Q9CZJ2; -.
DR MaxQB; Q9CZJ2; -.
DR PaxDb; Q9CZJ2; -.
DR PeptideAtlas; Q9CZJ2; -.
DR PRIDE; Q9CZJ2; -.
DR ProteomicsDB; 273383; -.
DR Antibodypedia; 2863; 129 antibodies from 24 providers.
DR Ensembl; ENSMUST00000099349; ENSMUSP00000096950; ENSMUSG00000074793.
DR GeneID; 72630; -.
DR KEGG; mmu:72630; -.
DR UCSC; uc008mkr.1; mouse.
DR CTD; 116835; -.
DR MGI; MGI:1919880; Hspa12b.
DR VEuPathDB; HostDB:ENSMUSG00000074793; -.
DR eggNOG; KOG0101; Eukaryota.
DR GeneTree; ENSGT00940000159032; -.
DR HOGENOM; CLU_009958_5_3_1; -.
DR InParanoid; Q9CZJ2; -.
DR OMA; IYKHTME; -.
DR OrthoDB; 804087at2759; -.
DR PhylomeDB; Q9CZJ2; -.
DR TreeFam; TF329492; -.
DR Reactome; R-MMU-3371453; Regulation of HSF1-mediated heat shock response.
DR BioGRID-ORCS; 72630; 1 hit in 72 CRISPR screens.
DR ChiTaRS; Hspa12b; mouse.
DR PRO; PR:Q9CZJ2; -.
DR Proteomes; UP000000589; Chromosome 2.
DR RNAct; Q9CZJ2; protein.
DR Bgee; ENSMUSG00000074793; Expressed in aortic valve and 160 other tissues.
DR ExpressionAtlas; Q9CZJ2; baseline and differential.
DR Genevisible; Q9CZJ2; MM.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR InterPro; IPR043129; ATPase_NBD.
DR InterPro; IPR029509; HSPA12B.
DR PANTHER; PTHR14187:SF39; PTHR14187:SF39; 1.
DR SUPFAM; SSF53067; SSF53067; 2.
PE 1: Evidence at protein level;
KW ATP-binding; Direct protein sequencing; Nucleotide-binding; Phosphoprotein;
KW Reference proteome.
FT CHAIN 1..685
FT /note="Heat shock 70 kDa protein 12B"
FT /id="PRO_0000078295"
FT REGION 1..50
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 30..50
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 25
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q96MM6"
FT MOD_RES 29
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q96MM6"
FT MOD_RES 42
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:17242355,
FT ECO:0007744|PubMed:21183079"
FT MOD_RES 44
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 46
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 276
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q96MM6"
SQ SEQUENCE 685 AA; 76119 MW; 641FE753180EBE2B CRC64;
MLTVPEMGLQ GLYISSSPER SPVPSPPGSP RTQESCGIAP LTPSQSPKPE ARALQQASFS
VVVAIDFGTT SSGYAFSFAS DPEAIHMMRK WEGGDPGVAH QKTPTCLLLT PEGIFHSFGY
TARDYYHDLD PEEARDWLYF EKFKMKIHSA TDLTLKTQLE AVNGKKMLAL EVFAHALRFF
KEHALQELRE QSECMLEKGA VRWVLTVPAI WKQPAKQFMR EAAYLAGLVS REDAEKLLIA
LEPEAASVYC RKLRLHQLMD LSSRTAGRGR LGERRSIDSS FRHAREQLRR SRHSRTFLVE
AGVGELWAEM QEGDRYMVAD CGGGTVDLTV HQLEQPHGTL KELYKASGGP YGAVGVDLAF
EQLLCRIFGE DFIAKFKRQR PAAWVDLTIA FEARKRTAGP HRAGALNISL PFSFIDFYRK
QRGHNVETAL RRSSVNLVKW SSQGMLRMSC EAMNELFQPT VSGIIQHIEM LLAKPEVQGV
KLLFLVGGFA ESAVLQHAVQ EALGTRGLRV VVPHDVGLTI LKGAVLFGQA PGVVRVRRSP
LTYGVGVLNR FVPGHHPPEK LLVRDGRRWC TDVFERFVAA EQSVALGEEV RRSYCPARPG
QRRVLINLYC CAAEDARFIT DPGVRKCGAL SLELEPEGCP ENTGTSPSRR EIRAAMQFGD
TEIKVTAVDV STNRSVRAAI DFLSN
