ID   AP2S_DICDI              Reviewed;         142 AA.
AC   Q54H39;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   18-MAR-2008, sequence version 2.
DT   25-MAY-2022, entry version 90.
DE   RecName: Full=AP-2 complex subunit sigma;
DE   AltName: Full=Adaptin small chain;
DE   AltName: Full=Adaptor-related protein complex 2 subunit sigma;
DE   AltName: Full=Clathrin assembly protein 2 sigma small chain;
DE   AltName: Full=Sigma2-adaptin;
GN   Name=ap2s1; ORFNames=DDB_G0289721;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- FUNCTION: Component of the adaptor complexes which link clathrin to
CC       receptors in coated vesicles. Clathrin-associated protein complexes are
CC       believed to interact with the cytoplasmic tails of membrane proteins,
CC       leading to their selection and concentration (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Adaptor protein complex 2 (AP-2) is a heterotetramer composed
CC       of two large adaptins (alpha-type and beta-type subunits), a medium
CC       adaptin (mu-type subunit AP50) and a small adaptin (sigma-type subunit
CC       AP17).
CC   -!- SUBCELLULAR LOCATION: Cell membrane. Membrane, coated pit
CC       {ECO:0000250}; Peripheral membrane protein {ECO:0000250}; Cytoplasmic
CC       side {ECO:0000250}. Note=Component of the coat surrounding the
CC       cytoplasmic face of coated vesicles in the plasma membrane.
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the adaptor complexes small subunit family.
CC       {ECO:0000305}.
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DR   EMBL; AAFI02000148; EAL62572.2; -; Genomic_DNA.
DR   RefSeq; XP_636079.2; XM_630987.2.
DR   AlphaFoldDB; Q54H39; -.
DR   SMR; Q54H39; -.
DR   STRING; 44689.DDB0234236; -.
DR   PaxDb; Q54H39; -.
DR   EnsemblProtists; EAL62572; EAL62572; DDB_G0289721.
DR   GeneID; 8627289; -.
DR   KEGG; ddi:DDB_G0289721; -.
DR   dictyBase; DDB_G0289721; ap2s1.
DR   eggNOG; KOG0935; Eukaryota.
DR   HOGENOM; CLU_061221_3_2_1; -.
DR   InParanoid; Q54H39; -.
DR   OMA; IYLSHIH; -.
DR   PhylomeDB; Q54H39; -.
DR   Reactome; R-DDI-437239; Recycling pathway of L1.
DR   Reactome; R-DDI-8856825; Cargo recognition for clathrin-mediated endocytosis.
DR   Reactome; R-DDI-8856828; Clathrin-mediated endocytosis.
DR   Reactome; R-DDI-8866427; VLDLR internalisation and degradation.
DR   Reactome; R-DDI-8964038; LDL clearance.
DR   PRO; PR:Q54H39; -.
DR   Proteomes; UP000002195; Chromosome 5.
DR   GO; GO:0030122; C:AP-2 adaptor complex; IDA:dictyBase.
DR   GO; GO:0030132; C:clathrin coat of coated pit; IC:dictyBase.
DR   GO; GO:0043231; C:intracellular membrane-bounded organelle; IBA:GO_Central.
DR   GO; GO:0035615; F:clathrin adaptor activity; IEA:InterPro.
DR   GO; GO:0072583; P:clathrin-dependent endocytosis; IEA:InterPro.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   GO; GO:0016192; P:vesicle-mediated transport; IBA:GO_Central.
DR   CDD; cd14833; AP2_sigma; 1.
DR   InterPro; IPR016635; AP_complex_ssu.
DR   InterPro; IPR022775; AP_mu_sigma_su.
DR   InterPro; IPR027156; APS2.
DR   InterPro; IPR011012; Longin-like_dom_sf.
DR   PANTHER; PTHR11753; PTHR11753; 1.
DR   PANTHER; PTHR11753:SF6; PTHR11753:SF6; 1.
DR   Pfam; PF01217; Clat_adaptor_s; 1.
DR   PIRSF; PIRSF015588; AP_complex_sigma; 1.
DR   SUPFAM; SSF64356; SSF64356; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Coated pit; Endocytosis; Membrane; Protein transport;
KW   Reference proteome; Transport.
FT   CHAIN           1..142
FT                   /note="AP-2 complex subunit sigma"
FT                   /id="PRO_0000328667"
SQ   SEQUENCE   142 AA;  17016 MW;  556A88DBA8ED8D7F CRC64;
     MIHFILIQNR QGKTRLSKWY TPYEDVEKRK LSHEIHKIVN SRETKFTNFV EFRTHRIVYR
     RYAGLFFSVC VDPTDNELFC LEAIHLFVEV LDAYFGNVCE LDLVFNFYKV YAIIDEVFLA
     GELMEPSKHV ILQRMEFLDN LP