HSD4A_ARATH
ID HSD4A_ARATH Reviewed; 299 AA.
AC P0DKC7; Q9FGP2;
DT 01-MAY-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2013, sequence version 1.
DT 03-AUG-2022, entry version 51.
DE RecName: Full=11-beta-hydroxysteroid dehydrogenase-like 4A;
DE EC=1.1.1.-;
DE AltName: Full=17-beta-hydroxysteroid dehydrogenase-like 4A;
DE EC=1.1.1.-;
DE AltName: Full=Hydroxysteroid dehydrogenase 4;
DE Short=AtHSD4;
GN Name=HSD4; OrderedLocusNames=At5g50590; ORFNames=MBA10.15;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RA Kaneko T., Katoh T., Asamizu E., Sato S., Nakamura Y., Kotani H.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. XI.";
RL Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Underwood B.A., Xiao Y.-L., Moskal W.A. Jr., Monaghan E.L., Wang W.,
RA Redman J.C., Wu H.C., Utterback T., Town C.D.;
RL Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Quinitio C., Chen H., Kim C.J., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP GENE FAMILY.
RX PubMed=17616511; DOI=10.1104/pp.107.100560;
RA Li F., Asami T., Wu X., Tsang E.W., Cutler A.J.;
RT "A putative hydroxysteroid dehydrogenase involved in regulating plant
RT growth and development.";
RL Plant Physiol. 145:87-97(2007).
RN [6]
RP GENE FAMILY.
RX PubMed=19542545; DOI=10.1093/pcp/pcp092;
RA Baud S., Dichow N.R., Kelemen Z., d'Andrea S., To A., Berger N.,
RA Canonge M., Kronenberger J., Viterbo D., Dubreucq B., Lepiniec L.,
RA Chardot T., Miquel M.;
RT "Regulation of HSD1 in seeds of Arabidopsis thaliana.";
RL Plant Cell Physiol. 50:1463-1478(2009).
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass type II
CC membrane protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR)
CC family. {ECO:0000305}.
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DR EMBL; AB025619; BAB09144.1; -; Genomic_DNA.
DR EMBL; CP002688; AED95966.1; -; Genomic_DNA.
DR EMBL; DQ056713; AAY78859.1; -; mRNA.
DR EMBL; BT026066; ABG48422.1; -; mRNA.
DR RefSeq; NP_199871.1; NM_124447.2.
DR RefSeq; NP_680417.1; NM_148112.2.
DR AlphaFoldDB; P0DKC7; -.
DR SMR; P0DKC7; -.
DR BioGRID; 20374; 1.
DR BioGRID; 20386; 1.
DR STRING; 3702.AT5G50590.1; -.
DR PaxDb; P0DKC7; -.
DR PRIDE; P0DKC7; -.
DR EnsemblPlants; AT5G50590.1; AT5G50590.1; AT5G50590.
DR EnsemblPlants; AT5G50690.1; AT5G50690.1; AT5G50690.
DR GeneID; 835128; -.
DR GeneID; 835140; -.
DR Gramene; AT5G50590.1; AT5G50590.1; AT5G50590.
DR Gramene; AT5G50690.1; AT5G50690.1; AT5G50690.
DR KEGG; ath:AT5G50590; -.
DR KEGG; ath:AT5G50690; -.
DR Araport; AT5G50590; -.
DR eggNOG; KOG1205; Eukaryota.
DR HOGENOM; CLU_010194_2_1_1; -.
DR InParanoid; P0DKC7; -.
DR OrthoDB; 906746at2759; -.
DR PhylomeDB; P0DKC7; -.
DR PRO; PR:P0DKC7; -.
DR Proteomes; UP000006548; Chromosome 5.
DR Genevisible; P0DKC7; AT.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0072582; F:17-beta-hydroxysteroid dehydrogenase (NADP+) activity; IBA:GO_Central.
DR GO; GO:0016491; F:oxidoreductase activity; IBA:GO_Central.
DR GO; GO:0006694; P:steroid biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:0008202; P:steroid metabolic process; IBA:GO_Central.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR020904; Sc_DH/Rdtase_CS.
DR InterPro; IPR002347; SDR_fam.
DR Pfam; PF00106; adh_short; 1.
DR PRINTS; PR00081; GDHRDH.
DR PRINTS; PR00080; SDRFAMILY.
DR SUPFAM; SSF51735; SSF51735; 1.
DR PROSITE; PS00061; ADH_SHORT; 1.
PE 2: Evidence at transcript level;
KW Lipid biosynthesis; Lipid metabolism; Membrane; NADP; Oxidoreductase;
KW Reference proteome; Signal-anchor; Steroid biosynthesis; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..299
FT /note="11-beta-hydroxysteroid dehydrogenase-like 4A"
FT /id="PRO_0000422282"
FT TRANSMEM 10..30
FT /note="Helical; Signal-anchor for type II membrane protein"
FT /evidence="ECO:0000255"
FT ACT_SITE 197
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10001"
FT BINDING 54..80
FT /ligand="NADP(+)"
FT /ligand_id="ChEBI:CHEBI:58349"
FT /evidence="ECO:0000250"
FT BINDING 105
FT /ligand="NADP(+)"
FT /ligand_id="ChEBI:CHEBI:58349"
FT /evidence="ECO:0000250"
FT BINDING 184
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 197..201
FT /ligand="NADP(+)"
FT /ligand_id="ChEBI:CHEBI:58349"
FT /evidence="ECO:0000250"
FT BINDING 201
FT /ligand="NADP(+)"
FT /ligand_id="ChEBI:CHEBI:58349"
FT /evidence="ECO:0000250"
SQ SEQUENCE 299 AA; 33248 MW; 77F6AC14AD01DD9D CRC64;
MDLNNKIFNI LLPIVTVSFL LVFMPFSIFF KLLQFIRGCK ESEKVNGKVV IITGSSSGIG
EHLAYEYARR GAYLTLVARR EDRLQVVADR CRKLGSPDVA VVRGDVSVIK DCKRFVQETI
SRFGRLDHLV NNAGIAEAKF FEDYSEISDV LPIVNTNFWG PVYATHFAIP HLKKTKGKII
AVASPAGWSG VPRMSIYAAS KAAMINFYET LRIELHPEVG VTIVFPGLIE NGNTNPDLLA
EKQDWSQVVT IESAAECAKA VVNGICRGKT FVAEPSWVRV LFWLSAICPE LLISKPKRN
