HSD6_ARATH
ID HSD6_ARATH Reviewed; 342 AA.
AC Q9LUE4;
DT 01-MAY-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=11-beta-hydroxysteroid dehydrogenase-like 6;
DE EC=1.1.1.-;
DE AltName: Full=17-beta-hydroxysteroid dehydrogenase-like 6;
DE EC=1.1.1.-;
DE AltName: Full=Hydroxysteroid dehydrogenase 6;
DE Short=AtHSD6;
GN Name=HSD6; OrderedLocusNames=At5g50770; ORFNames=MFB16.17;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10718197; DOI=10.1093/dnares/7.1.31;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence
RT features of the regions of 3,076,755 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:31-63(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Shinn P., Chen H., Cheuk R., Kim C.J., Ecker J.R.;
RT "Arabidopsis cDNA clones.";
RL Submitted (JAN-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP GENE FAMILY.
RX PubMed=17616511; DOI=10.1104/pp.107.100560;
RA Li F., Asami T., Wu X., Tsang E.W., Cutler A.J.;
RT "A putative hydroxysteroid dehydrogenase involved in regulating plant
RT growth and development.";
RL Plant Physiol. 145:87-97(2007).
RN [5]
RP GENE FAMILY.
RX PubMed=19542545; DOI=10.1093/pcp/pcp092;
RA Baud S., Dichow N.R., Kelemen Z., d'Andrea S., To A., Berger N.,
RA Canonge M., Kronenberger J., Viterbo D., Dubreucq B., Lepiniec L.,
RA Chardot T., Miquel M.;
RT "Regulation of HSD1 in seeds of Arabidopsis thaliana.";
RL Plant Cell Physiol. 50:1463-1478(2009).
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass type II
CC membrane protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR)
CC family. {ECO:0000305}.
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DR EMBL; AB023037; BAA96990.1; -; Genomic_DNA.
DR EMBL; CP002688; AED95990.1; -; Genomic_DNA.
DR EMBL; BT020525; AAW49294.1; -; mRNA.
DR RefSeq; NP_199890.1; NM_124455.2.
DR AlphaFoldDB; Q9LUE4; -.
DR SMR; Q9LUE4; -.
DR STRING; 3702.AT5G50770.1; -.
DR PaxDb; Q9LUE4; -.
DR PRIDE; Q9LUE4; -.
DR ProteomicsDB; 232109; -.
DR EnsemblPlants; AT5G50770.1; AT5G50770.1; AT5G50770.
DR GeneID; 835149; -.
DR Gramene; AT5G50770.1; AT5G50770.1; AT5G50770.
DR KEGG; ath:AT5G50770; -.
DR Araport; AT5G50770; -.
DR TAIR; locus:2163315; AT5G50770.
DR eggNOG; KOG1205; Eukaryota.
DR HOGENOM; CLU_010194_2_1_1; -.
DR InParanoid; Q9LUE4; -.
DR OMA; ASGCGYI; -.
DR OrthoDB; 906746at2759; -.
DR PhylomeDB; Q9LUE4; -.
DR PRO; PR:Q9LUE4; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9LUE4; baseline and differential.
DR Genevisible; Q9LUE4; AT.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016491; F:oxidoreductase activity; IBA:GO_Central.
DR GO; GO:0006694; P:steroid biosynthetic process; IEA:UniProtKB-KW.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR020904; Sc_DH/Rdtase_CS.
DR InterPro; IPR002347; SDR_fam.
DR Pfam; PF00106; adh_short; 1.
DR PRINTS; PR00081; GDHRDH.
DR PRINTS; PR00080; SDRFAMILY.
DR SUPFAM; SSF51735; SSF51735; 1.
DR PROSITE; PS00061; ADH_SHORT; 1.
PE 2: Evidence at transcript level;
KW Lipid biosynthesis; Lipid metabolism; Membrane; NADP; Oxidoreductase;
KW Reference proteome; Signal-anchor; Steroid biosynthesis; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..342
FT /note="11-beta-hydroxysteroid dehydrogenase-like 6"
FT /id="PRO_0000422285"
FT TRANSMEM 10..30
FT /note="Helical; Signal-anchor for type II membrane protein"
FT /evidence="ECO:0000255"
FT ACT_SITE 197
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10001"
FT BINDING 54..80
FT /ligand="NADP(+)"
FT /ligand_id="ChEBI:CHEBI:58349"
FT /evidence="ECO:0000250"
FT BINDING 105
FT /ligand="NADP(+)"
FT /ligand_id="ChEBI:CHEBI:58349"
FT /evidence="ECO:0000250"
FT BINDING 184
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 197..201
FT /ligand="NADP(+)"
FT /ligand_id="ChEBI:CHEBI:58349"
FT /evidence="ECO:0000250"
FT BINDING 201
FT /ligand="NADP(+)"
FT /ligand_id="ChEBI:CHEBI:58349"
FT /evidence="ECO:0000250"
SQ SEQUENCE 342 AA; 37993 MW; 45ADF1559AAD97FC CRC64;
MDSINKIINF LFPLLTLYAL LVFYPTYQRL KSAVSICRNL FSENVAGKVV VITGAASGIG
EALAYEYGKR GAYLALVDIR GEPLFHVAAL AELYGSPEVL PLVADVSKLQ DCERFIRATV
LHFGRLDHLV TNAGVAPLYF FADIEDVSKA SPAMDINFWG SVYCTFFASP YLKKFRGRIV
VIASGCGYIA SPRLSFYCAS KAAVIAFYET LRTEFGSDIG VTIVAPGIVD SEMSRGKFMT
KDGKLVVDKE LRDVQMSVLP VESAERCAKA IMRSVCRGDR YLLEPDWIGC VILLKVFCSE
ATEWVARWLL IARPKFPMME ALSNKILDVA HAFNSFFSFP HY
