ID   HSDL1_RAT               Reviewed;         330 AA.
AC   Q4V8B7;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2005, sequence version 1.
DT   03-AUG-2022, entry version 117.
DE   RecName: Full=Inactive hydroxysteroid dehydrogenase-like protein 1;
GN   Name=Hsdl1;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Testis;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- SUBUNIT: Interacts with STYXL1. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR)
CC       family. 17-beta-HSD 3 subfamily. {ECO:0000305}.
CC   -!- CAUTION: Although it belongs to the SDR family, Phe-218 is present
CC       instead of the conserved Tyr which is an active site residue. It is
CC       therefore expected that this protein lacks oxidoreductase activity.
CC       {ECO:0000305}.
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DR   EMBL; BC097457; AAH97457.1; -; mRNA.
DR   RefSeq; NP_001020067.1; NM_001024896.1.
DR   RefSeq; XP_006255775.1; XM_006255713.3.
DR   RefSeq; XP_006255776.1; XM_006255714.3.
DR   AlphaFoldDB; Q4V8B7; -.
DR   SMR; Q4V8B7; -.
DR   IntAct; Q4V8B7; 1.
DR   STRING; 10116.ENSRNOP00000020860; -.
DR   SwissPalm; Q4V8B7; -.
DR   jPOST; Q4V8B7; -.
DR   PaxDb; Q4V8B7; -.
DR   PRIDE; Q4V8B7; -.
DR   Ensembl; ENSRNOT00000114729; ENSRNOP00000088924; ENSRNOG00000015576.
DR   GeneID; 361418; -.
DR   KEGG; rno:361418; -.
DR   UCSC; RGD:1308433; rat.
DR   CTD; 83693; -.
DR   RGD; 1308433; Hsdl1.
DR   eggNOG; KOG1014; Eukaryota.
DR   GeneTree; ENSGT00940000160053; -.
DR   HOGENOM; CLU_010194_38_0_1; -.
DR   InParanoid; Q4V8B7; -.
DR   OMA; SCNCYVE; -.
DR   OrthoDB; 913128at2759; -.
DR   PhylomeDB; Q4V8B7; -.
DR   TreeFam; TF314591; -.
DR   PRO; PR:Q4V8B7; -.
DR   Proteomes; UP000002494; Chromosome 19.
DR   Bgee; ENSRNOG00000015576; Expressed in skeletal muscle tissue and 18 other tissues.
DR   GO; GO:0045111; C:intermediate filament cytoskeleton; IEA:Ensembl.
DR   GO; GO:0005739; C:mitochondrion; ISS:UniProtKB.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR002347; SDR_fam.
DR   Pfam; PF00106; adh_short; 1.
DR   PRINTS; PR00081; GDHRDH.
DR   SUPFAM; SSF51735; SSF51735; 1.
PE   2: Evidence at transcript level;
KW   Acetylation; Mitochondrion; NADP; Reference proteome.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000250|UniProtKB:Q3SXM5"
FT   CHAIN           2..330
FT                   /note="Inactive hydroxysteroid dehydrogenase-like protein
FT                   1"
FT                   /id="PRO_0000313674"
FT   REGION          2..82
FT                   /note="Required for mitochondria translocation"
FT                   /evidence="ECO:0000250"
FT   BINDING         74..80
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250"
FT   BINDING         125
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250"
FT   BINDING         222
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250"
FT   MOD_RES         2
FT                   /note="N-acetylalanine"
FT                   /evidence="ECO:0000250|UniProtKB:Q3SXM5"
SQ   SEQUENCE   330 AA;  36908 MW;  2596B7EAE1337244 CRC64;
     MAAVDSFYLL YREIARSCSC YVEALALVGA WYTARKSIPV ICDFYSLVRL HFIPRLGSRP
     DLIKQYGRWA VISGATDGIG KAYAEELASH GLNIILISQE EEKLQAVAKH IADTYRVETL
     VLVADFSRGR EIYAPIREAL RDRDIGILVN DVGAFYPYPQ YFSQVPEDTI WDIVNVNIAA
     ASLMVHIVLP GMVERKKGAI VTVSSGSCCK PTPQLAAFSA SKAYLDHFSR ALQYEYASKG
     IFVQSLIPFY VTSSVTAPGS FLRRCPWLAP SPRVYAQHAV STLGISKRTT GYWSHSIQFL
     FAQYMPEWLW VWGANLLNRS LRKEALSSQA