AP2S_YEAST
ID AP2S_YEAST Reviewed; 147 AA.
AC Q00381; D6VWM9;
DT 01-APR-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-APR-1993, sequence version 1.
DT 03-AUG-2022, entry version 168.
DE RecName: Full=AP-2 complex subunit sigma;
DE AltName: Full=Adaptin small chain;
DE AltName: Full=Clathrin assembly protein 2 sigma small chain;
DE AltName: Full=Clathrin coat assembly protein AP17;
DE AltName: Full=Clathrin coat-associated protein AP17;
DE AltName: Full=Plasma membrane adaptor AP-2 17 kDa protein;
DE AltName: Full=Sigma2-adaptin;
GN Name=APS2; Synonyms=YAP17; OrderedLocusNames=YJR058C; ORFNames=J1720;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2040623; DOI=10.1016/s0021-9258(18)99141-6;
RA Kirchhausen T., Davis A.C., Frucht S., O'Brine Greco B., Payne G.S.,
RA Tubb B.;
RT "AP17 and AP19, the mammalian small chains of the clathrin-associated
RT protein complexes show homology to Yap17p, their putative homolog in
RT yeast.";
RL J. Biol. Chem. 266:11153-11157(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8840504;
RX DOI=10.1002/(sici)1097-0061(199607)12:9<869::aid-yea964>3.0.co;2-1;
RA Huang M.-E., Manus V., Chuat J.-C., Galibert F.;
RT "Analysis of a 62 kb DNA sequence of chromosome X reveals 36 open reading
RT frames and a gene cluster with a counterpart on chromosome XI.";
RL Yeast 12:869-875(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8641269; DOI=10.1002/j.1460-2075.1996.tb00557.x;
RA Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C.,
RA Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D.,
RA Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J.,
RA Heumann K., Hilger F., Hollenberg C.P., Huang M.-E., Jacq C.,
RA Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E.,
RA Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T.,
RA Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B.,
RA Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R.,
RA Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N.,
RA To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H.,
RA von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.;
RT "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.";
RL EMBO J. 15:2031-2049(1996).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [6]
RP FUNCTION, SUBUNIT, AND INTERACTION WITH APL1.
RX PubMed=10564262; DOI=10.1091/mbc.10.11.3643;
RA Yeung B.G., Phan H.L., Payne G.S.;
RT "Adaptor complex-independent clathrin function in yeast.";
RL Mol. Biol. Cell 10:3643-3659(1999).
RN [7]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
CC -!- FUNCTION: Component of the adaptor complexes which link clathrin to
CC receptors in coated vesicles. Clathrin-associated protein complexes are
CC believed to interact with the cytoplasmic tails of membrane proteins,
CC leading to their selection and concentration.
CC {ECO:0000269|PubMed:10564262}.
CC -!- SUBUNIT: Adaptor protein complex 2 (AP-2) is a heterotetramer composed
CC of two large adaptins (alpha-type subunit APL3 and beta-type subunit
CC APL1), a medium chain (mu-type subunit APM4) and a small adaptin
CC (sigma-type subunit APS2). Interacts with APL1.
CC {ECO:0000269|PubMed:10564262}.
CC -!- INTERACTION:
CC Q00381; P38065: APL3; NbExp=3; IntAct=EBI-2608, EBI-2181;
CC -!- SUBCELLULAR LOCATION: Cell membrane. Membrane, coated pit; Peripheral
CC membrane protein; Cytoplasmic side. Note=Component of the coat
CC surrounding the cytoplasmic face of the plasma membrane coated
CC vesicles.
CC -!- MISCELLANEOUS: Present with 1390 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the adaptor complexes small subunit family.
CC {ECO:0000305}.
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DR EMBL; M37193; AAA35225.1; -; Genomic_DNA.
DR EMBL; Z49558; CAA89586.1; -; Genomic_DNA.
DR EMBL; L47993; AAB39284.1; -; Genomic_DNA.
DR EMBL; AY557893; AAS56219.1; -; Genomic_DNA.
DR EMBL; BK006943; DAA08845.1; -; Genomic_DNA.
DR PIR; C40535; C40535.
DR RefSeq; NP_012592.1; NM_001181716.1.
DR AlphaFoldDB; Q00381; -.
DR SMR; Q00381; -.
DR BioGRID; 33815; 50.
DR ComplexPortal; CPX-534; Adapter complex AP-2.
DR DIP; DIP-5093N; -.
DR IntAct; Q00381; 6.
DR MINT; Q00381; -.
DR STRING; 4932.YJR058C; -.
DR MaxQB; Q00381; -.
DR PaxDb; Q00381; -.
DR PRIDE; Q00381; -.
DR EnsemblFungi; YJR058C_mRNA; YJR058C; YJR058C.
DR GeneID; 853521; -.
DR KEGG; sce:YJR058C; -.
DR SGD; S000003819; APS2.
DR VEuPathDB; FungiDB:YJR058C; -.
DR eggNOG; KOG0935; Eukaryota.
DR GeneTree; ENSGT00970000193421; -.
DR HOGENOM; CLU_061221_3_1_1; -.
DR InParanoid; Q00381; -.
DR OMA; IYLSHIH; -.
DR BioCyc; YEAST:G3O-31691-MON; -.
DR Reactome; R-SCE-437239; Recycling pathway of L1.
DR Reactome; R-SCE-8856825; Cargo recognition for clathrin-mediated endocytosis.
DR Reactome; R-SCE-8856828; Clathrin-mediated endocytosis.
DR Reactome; R-SCE-8866427; VLDLR internalisation and degradation.
DR Reactome; R-SCE-8964038; LDL clearance.
DR PRO; PR:Q00381; -.
DR Proteomes; UP000002311; Chromosome X.
DR RNAct; Q00381; protein.
DR GO; GO:0030122; C:AP-2 adaptor complex; IDA:SGD.
DR GO; GO:0005935; C:cellular bud neck; HDA:SGD.
DR GO; GO:0030136; C:clathrin-coated vesicle; IDA:SGD.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; HDA:SGD.
DR GO; GO:0005628; C:prospore membrane; HDA:SGD.
DR GO; GO:0035615; F:clathrin adaptor activity; IEA:InterPro.
DR GO; GO:0072583; P:clathrin-dependent endocytosis; IEA:InterPro.
DR GO; GO:0006886; P:intracellular protein transport; IC:SGD.
DR GO; GO:0016192; P:vesicle-mediated transport; IBA:GO_Central.
DR InterPro; IPR016635; AP_complex_ssu.
DR InterPro; IPR022775; AP_mu_sigma_su.
DR InterPro; IPR027156; APS2.
DR InterPro; IPR000804; Clathrin_sm-chain_CS.
DR InterPro; IPR011012; Longin-like_dom_sf.
DR PANTHER; PTHR11753; PTHR11753; 1.
DR PANTHER; PTHR11753:SF6; PTHR11753:SF6; 1.
DR Pfam; PF01217; Clat_adaptor_s; 1.
DR PIRSF; PIRSF015588; AP_complex_sigma; 1.
DR SUPFAM; SSF64356; SSF64356; 1.
DR PROSITE; PS00989; CLAT_ADAPTOR_S; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Coated pit; Endocytosis; Membrane; Protein transport;
KW Reference proteome; Transport.
FT CHAIN 1..147
FT /note="AP-2 complex subunit sigma"
FT /id="PRO_0000193814"
SQ SEQUENCE 147 AA; 17373 MW; DFFD1A567532AAD8 CRC64;
MAVQFILCFN KQGVVRLVRW FDVHSSDPQR SQDAIAQIYR LISSRDHKHQ SNFVEFSDST
KLIYRRYAGL YFVMGVDLLD DEPIYLCHIH LFVEVLDAFF GNVCELDIVF NFYKVYMIMD
EMFIGGEIQE ISKDMLLERL SILDRLD
