ID   HSD_MYCTO               Reviewed;         260 AA.
AC   P9WGT0; L0TB81; P69167; Q10855;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   03-AUG-2022, entry version 41.
DE   RecName: Full=3-alpha-(or 20-beta)-hydroxysteroid dehydrogenase {ECO:0000250|UniProtKB:P9WGT1};
DE            EC=1.1.1.53 {ECO:0000250|UniProtKB:P9WGT1};
GN   Name=fabG3; OrderedLocusNames=MT2058;
OS   Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83331;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CDC 1551 / Oshkosh;
RX   PubMed=12218036; DOI=10.1128/jb.184.19.5479-5490.2002;
RA   Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O.,
RA   Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K.,
RA   Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L.,
RA   Delcher A., Utterback T.R., Weidman J.F., Khouri H.M., Gill J., Mikula A.,
RA   Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.;
RT   "Whole-genome comparison of Mycobacterium tuberculosis clinical and
RT   laboratory strains.";
RL   J. Bacteriol. 184:5479-5490(2002).
CC   -!- FUNCTION: Probably involved in steroid metabolism.
CC       {ECO:0000250|UniProtKB:P9WGT1}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=androstan-3alpha,17beta-diol + NAD(+) = 17beta-
CC         hydroxyandrostanone + H(+) + NADH; Xref=Rhea:RHEA:22400,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:18011, ChEBI:CHEBI:57540,
CC         ChEBI:CHEBI:57945, ChEBI:CHEBI:85278; EC=1.1.1.53;
CC         Evidence={ECO:0000250|UniProtKB:P9WGT1};
CC   -!- PATHWAY: Lipid metabolism; steroid degradation.
CC       {ECO:0000250|UniProtKB:P9WGT1}.
CC   -!- SUBUNIT: Homotetramer. {ECO:0000250|UniProtKB:P9WGT1}.
CC   -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR)
CC       family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AE000516; AAK46335.1; -; Genomic_DNA.
DR   PIR; H70758; H70758.
DR   RefSeq; WP_003899124.1; NZ_KK341227.1.
DR   AlphaFoldDB; P9WGT0; -.
DR   SMR; P9WGT0; -.
DR   EnsemblBacteria; AAK46335; AAK46335; MT2058.
DR   KEGG; mtc:MT2058; -.
DR   PATRIC; fig|83331.31.peg.2215; -.
DR   HOGENOM; CLU_010194_1_0_11; -.
DR   UniPathway; UPA00722; -.
DR   Proteomes; UP000001020; Chromosome.
DR   GO; GO:0047044; F:androstan-3-alpha,17-beta-diol dehydrogenase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006706; P:steroid catabolic process; IEA:UniProtKB-UniPathway.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR020904; Sc_DH/Rdtase_CS.
DR   InterPro; IPR002347; SDR_fam.
DR   PRINTS; PR00081; GDHRDH.
DR   PRINTS; PR00080; SDRFAMILY.
DR   SUPFAM; SSF51735; SSF51735; 1.
DR   PROSITE; PS00061; ADH_SHORT; 1.
PE   3: Inferred from homology;
KW   Lipid metabolism; NAD; Oxidoreductase; Steroid metabolism.
FT   CHAIN           1..260
FT                   /note="3-alpha-(or 20-beta)-hydroxysteroid dehydrogenase"
FT                   /id="PRO_0000428308"
FT   ACT_SITE        153
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250|UniProtKB:P9WGT1"
FT   BINDING         17..19
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250|UniProtKB:P9WGT1"
FT   BINDING         38
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250|UniProtKB:P9WGT1"
FT   BINDING         61..62
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250|UniProtKB:P9WGT1"
FT   BINDING         88
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250|UniProtKB:P9WGT1"
FT   BINDING         153
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250|UniProtKB:P9WGT1"
FT   BINDING         157
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250|UniProtKB:P9WGT1"
FT   BINDING         183..191
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250|UniProtKB:P9WGT1"
SQ   SEQUENCE   260 AA;  27000 MW;  1C74F55B87623A77 CRC64;
     MSGRLIGKVA LVSGGARGMG ASHVRAMVAE GAKVVFGDIL DEEGKAVAAE LADAARYVHL
     DVTQPAQWTA AVDTAVTAFG GLHVLVNNAG ILNIGTIEDY ALTEWQRILD VNLTGVFLGI
     RAVVKPMKEA GRGSIINISS IEGLAGTVAC HGYTATKFAV RGLTKSTALE LGPGGIRVNS
     IHPGLVKTPM TDWVPEDIFQ TALGRAAEPV EVSNLVVYLA SDESSYSTGA EFVVDGGTVA
     GLAHNDFGAV EVSSQPEWVT