ID   HSD_STREX               Reviewed;         255 AA.
AC   P19992;
DT   01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1991, sequence version 1.
DT   03-AUG-2022, entry version 108.
DE   RecName: Full=3-alpha-(or 20-beta)-hydroxysteroid dehydrogenase;
DE            EC=1.1.1.53;
OS   Streptomyces exfoliatus (Streptomyces hydrogenans).
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Streptomyces.
OX   NCBI_TaxID=1905;
RN   [1]
RP   PROTEIN SEQUENCE.
RX   PubMed=2194840; DOI=10.1016/0014-5793(90)81504-h;
RA   Marekov L., Krook M., Joernvall H.;
RT   "Prokaryotic 20 beta-hydroxysteroid dehydrogenase is an enzyme of the
RT   'short-chain, non-metalloenzyme' alcohol dehydrogenase type.";
RL   FEBS Lett. 266:51-54(1990).
RN   [2]
RP   X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS).
RX   PubMed=1946424; DOI=10.1073/pnas.88.22.10064;
RA   Ghosh D., Weeks C.M., Grochulski P., Duax W.L., Erman M., Rimsay R.L.,
RA   Orr J.C.;
RT   "Three-dimensional structure of holo 3 alpha,20 beta-hydroxysteroid
RT   dehydrogenase: a member of a short-chain dehydrogenase family.";
RL   Proc. Natl. Acad. Sci. U.S.A. 88:10064-10068(1991).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=androstan-3alpha,17beta-diol + NAD(+) = 17beta-
CC         hydroxyandrostanone + H(+) + NADH; Xref=Rhea:RHEA:22400,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:18011, ChEBI:CHEBI:57540,
CC         ChEBI:CHEBI:57945, ChEBI:CHEBI:85278; EC=1.1.1.53;
CC   -!- PATHWAY: Lipid metabolism; C21-steroid hormone metabolism.
CC   -!- SUBUNIT: Homotetramer.
CC   -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR)
CC       family. {ECO:0000305}.
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DR   PIR; S10707; S10707.
DR   PDB; 1HDC; X-ray; 2.20 A; A/B/C/D=2-255.
DR   PDB; 2HSD; X-ray; 2.64 A; A/B/C/D=2-255.
DR   PDBsum; 1HDC; -.
DR   PDBsum; 2HSD; -.
DR   AlphaFoldDB; P19992; -.
DR   SMR; P19992; -.
DR   ChEMBL; CHEMBL3243917; -.
DR   DrugBank; DB02329; Carbenoxolone.
DR   UniPathway; UPA00229; -.
DR   EvolutionaryTrace; P19992; -.
DR   GO; GO:0047044; F:androstan-3-alpha,17-beta-diol dehydrogenase activity; IEA:UniProtKB-EC.
DR   GO; GO:0008207; P:C21-steroid hormone metabolic process; IEA:UniProtKB-UniPathway.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR020904; Sc_DH/Rdtase_CS.
DR   InterPro; IPR002347; SDR_fam.
DR   PRINTS; PR00081; GDHRDH.
DR   PRINTS; PR00080; SDRFAMILY.
DR   SUPFAM; SSF51735; SSF51735; 1.
DR   PROSITE; PS00061; ADH_SHORT; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Direct protein sequencing; Lipid metabolism; NAD;
KW   Oxidoreductase; Steroid metabolism.
FT   CHAIN           1..255
FT                   /note="3-alpha-(or 20-beta)-hydroxysteroid dehydrogenase"
FT                   /id="PRO_0000054713"
FT   ACT_SITE        152
FT                   /note="Proton acceptor"
FT   BINDING         10..34
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT   BINDING         139
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   STRAND          7..12
FT                   /evidence="ECO:0007829|PDB:1HDC"
FT   TURN            13..15
FT                   /evidence="ECO:0007829|PDB:1HDC"
FT   HELIX           17..28
FT                   /evidence="ECO:0007829|PDB:1HDC"
FT   STRAND          32..38
FT                   /evidence="ECO:0007829|PDB:1HDC"
FT   HELIX           40..48
FT                   /evidence="ECO:0007829|PDB:1HDC"
FT   HELIX           49..53
FT                   /evidence="ECO:0007829|PDB:1HDC"
FT   STRAND          54..58
FT                   /evidence="ECO:0007829|PDB:1HDC"
FT   HELIX           64..78
FT                   /evidence="ECO:0007829|PDB:1HDC"
FT   STRAND          83..86
FT                   /evidence="ECO:0007829|PDB:1HDC"
FT   HELIX           96..98
FT                   /evidence="ECO:0007829|PDB:1HDC"
FT   HELIX           101..111
FT                   /evidence="ECO:0007829|PDB:1HDC"
FT   HELIX           113..129
FT                   /evidence="ECO:0007829|PDB:1HDC"
FT   STRAND          132..137
FT                   /evidence="ECO:0007829|PDB:1HDC"
FT   HELIX           140..142
FT                   /evidence="ECO:0007829|PDB:1HDC"
FT   HELIX           150..170
FT                   /evidence="ECO:0007829|PDB:1HDC"
FT   HELIX           171..173
FT                   /evidence="ECO:0007829|PDB:1HDC"
FT   STRAND          175..182
FT                   /evidence="ECO:0007829|PDB:1HDC"
FT   HELIX           188..193
FT                   /evidence="ECO:0007829|PDB:1HDC"
FT   HELIX           214..225
FT                   /evidence="ECO:0007829|PDB:1HDC"
FT   HELIX           227..229
FT                   /evidence="ECO:0007829|PDB:1HDC"
FT   STRAND          236..240
FT                   /evidence="ECO:0007829|PDB:1HDC"
FT   TURN            241..245
FT                   /evidence="ECO:0007829|PDB:1HDC"
FT   HELIX           249..253
FT                   /evidence="ECO:0007829|PDB:1HDC"
SQ   SEQUENCE   255 AA;  26484 MW;  9CB93CB66AA628D5 CRC64;
     MNDLSGKTVI ITGGARGLGA EAARQAVAAG ARVVLADVLD EEGAATAREL GDAARYQHLD
     VTIEEDWQRV VAYAREEFGS VDGLVNNAGI STGMFLETES VERFRKVVDI NLTGVFIGMK
     TVIPAMKDAG GGSIVNISSA AGLMGLALTS SYGASKWGVR GLSKLAAVEL GTDRIRVNSV
     HPGMTYTPMT AETGIRQGEG NYPNTPMGRV GNEPGEIAGA VVKLLSDTSS YVTGAELAVD
     GGWTTGPTVK YVMGQ