ID   AP2_GALME               Reviewed;          60 AA.
AC   P85216;
DT   21-AUG-2007, integrated into UniProtKB/Swiss-Prot.
DT   21-AUG-2007, sequence version 1.
DT   25-MAY-2022, entry version 20.
DE   RecName: Full=Anionic antimicrobial peptide 2;
OS   Galleria mellonella (Greater wax moth).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Lepidoptera; Glossata; Ditrysia; Pyraloidea;
OC   Pyralidae; Galleriinae; Galleria.
OX   NCBI_TaxID=7137;
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY,
RP   INDUCTION, AND MASS SPECTROMETRY.
RC   TISSUE=Larval hemolymph {ECO:0000269|PubMed:17194500};
RX   PubMed=17194500; DOI=10.1016/j.peptides.2006.11.010;
RA   Cytrynska M., Mak P., Zdybicka-Barabas A., Suder P., Jakubowicz T.;
RT   "Purification and characterization of eight peptides from Galleria
RT   mellonella immune hemolymph.";
RL   Peptides 28:533-546(2007).
CC   -!- FUNCTION: Antimicrobial protein. Has antibacterial activity against the
CC       Gram-positive bacteria M.luteus (MIC=86.6 uM), L.monocytogenes
CC       (MIC=86.6 uM), and S.lutea (MIC=86.6 uM). Lacks antibacterial activity
CC       against the Gram-positive bacteria B.circulans and S.aureus, and the
CC       Gram-negative bacteria E.coli D31, E.coli ATCC 25922, and
CC       S.typhimurium. Has antifungal activity against P.pastoris (MIC=86.6 uM)
CC       and P.stipitis (MIC=90.9 uM), but lacks antifungal activity against
CC       A.niger, C.albicans, C.albidus, C.fructus, C.wickerhamii, F.oxysporum,
CC       S.cerevisiae, S.pombe, T.harzianum, and Z.marxianus.
CC       {ECO:0000269|PubMed:17194500}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:17194500}.
CC   -!- TISSUE SPECIFICITY: Hemolymph. {ECO:0000269|PubMed:17194500}.
CC   -!- INDUCTION: By bacterial infection. {ECO:0000269|PubMed:17194500}.
CC   -!- MASS SPECTROMETRY: Mass=6978.9; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:17194500};
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DR   AlphaFoldDB; P85216; -.
DR   SMR; P85216; -.
DR   Proteomes; UP000504614; Unplaced.
DR   GO; GO:0005615; C:extracellular space; IDA:UniProtKB.
DR   GO; GO:0050832; P:defense response to fungus; IDA:UniProtKB.
DR   GO; GO:0050830; P:defense response to Gram-positive bacterium; IDA:UniProtKB.
DR   GO; GO:0045087; P:innate immune response; IDA:UniProtKB.
DR   GO; GO:0031640; P:killing of cells of another organism; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Antibiotic; Antimicrobial; Direct protein sequencing; Fungicide; Immunity;
KW   Innate immunity; Reference proteome; Secreted.
FT   PEPTIDE         1..60
FT                   /note="Anionic antimicrobial peptide 2"
FT                   /id="PRO_0000298765"
SQ   SEQUENCE   60 AA;  6980 MW;  A8BE57B003A4F182 CRC64;
     ETESTPDYLK NIQQQLEEYT KNFNTQVQNA FDSDKIKSEV NNFIESLGKI LNTEKKEAPK