ID   3SX3_MICCO              Reviewed;          82 AA.
AC   C6JUP3;
DT   26-JUN-2013, integrated into UniProtKB/Swiss-Prot.
DT   01-SEP-2009, sequence version 1.
DT   25-MAY-2022, entry version 24.
DE   RecName: Full=Three-finger toxin MicTx3 {ECO:0000303|PubMed:21214917};
DE   AltName: Full=MCOR0100C {ECO:0000303|PubMed:19291316, ECO:0000303|PubMed:21515432, ECO:0000312|EMBL:ACS74997.1};
DE   AltName: Full=Three-finger toxin 3FTx-3 {ECO:0000305};
DE   Flags: Precursor;
OS   Micrurus corallinus (Brazilian coral snake).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Lepidosauria; Squamata; Bifurcata; Unidentata; Episquamata; Toxicofera;
OC   Serpentes; Colubroidea; Elapidae; Elapinae; Micrurus.
OX   NCBI_TaxID=54390;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Venom gland;
RX   PubMed=19291316; DOI=10.1186/1471-2164-10-112;
RA   Leao L.I., Ho P.L., Junqueira-de-Azevedo I.L.M.;
RT   "Transcriptomic basis for an antiserum against Micrurus corallinus (coral
RT   snake) venom.";
RL   BMC Genomics 10:112-112(2009).
RN   [2]
RP   PROTEIN SEQUENCE OF 22-36, AND SUBCELLULAR LOCATION.
RC   TISSUE=Venom;
RX   PubMed=21515432; DOI=10.1016/j.jprot.2011.04.003;
RA   Correa-Netto C., Junqueira-de-Azevedo Ide L., Silva D.A., Ho P.L.,
RA   Leitao-de-Araujo M., Alves M.L., Sanz L., Foguel D., Zingali R.B.,
RA   Calvete J.J.;
RT   "Snake venomics and venom gland transcriptomic analysis of Brazilian coral
RT   snakes, Micrurus altirostris and M. corallinus.";
RL   J. Proteomics 74:1795-1809(2011).
RN   [3]
RP   FUNCTION.
RX   PubMed=21214917; DOI=10.1186/1756-6606-4-2;
RA   Naimuddin M., Kobayashi S., Tsutsui C., Machida M., Nemoto N., Sakai T.,
RA   Kubo T.;
RT   "Directed evolution of a three-finger neurotoxin by using cDNA display
RT   yields antagonists as well as agonists of interleukin-6 receptor
RT   signaling.";
RL   Mol. Brain 4:2-2(2011).
RN   [4]
RP   TRYPSIN INHIBITOR CREATION BY USING 3-FINGER TOXIN SCAFFOLD.
RX   PubMed=24308378; DOI=10.3109/10799893.2013.865747;
RA   Cai W., Naimuddin M., Inagaki H., Kameyama K., Ishida N., Kubo T.;
RT   "Directed evolution of three-finger toxin to produce serine protease
RT   inhibitors.";
RL   J. Recept. Signal Transduct. 34:154-161(2014).
CC   -!- FUNCTION: Has been described to inhibit nicotinic acetylcholine
CC       receptor (nAChR) alpha-7/CHRNA7 subunits and to bind acetylcholine
CC       binding protein (AChBP) (Kd=29.5 nM) (PubMed:21214917).
CC       {ECO:0000269|PubMed:21214917}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:19291316}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC       {ECO:0000305|PubMed:19291316}.
CC   -!- MISCELLANEOUS: Directed evolution was applied to create MicTx3 mutants
CC       that inhibit IL-6R and trypsin (PubMed:21214917, PubMed:24308378). It
CC       does not bind to interleukin-6 receptor (IL-6R) (PubMed:21214917). It
CC       probably does not inhibit trypsin (Probable).
CC       {ECO:0000269|PubMed:21214917, ECO:0000269|PubMed:24308378}.
CC   -!- SIMILARITY: Belongs to the snake three-finger toxin family. Short-chain
CC       subfamily. {ECO:0000305}.
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DR   EMBL; GQ139603; ACS74997.1; -; mRNA.
DR   AlphaFoldDB; C6JUP3; -.
DR   SMR; C6JUP3; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   Gene3D; 2.10.60.10; -; 1.
DR   InterPro; IPR045860; Snake_toxin-like_sf.
DR   SUPFAM; SSF57302; SSF57302; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Secreted; Signal; Toxin.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000269|PubMed:21515432"
FT   CHAIN           22..82
FT                   /note="Three-finger toxin MicTx3"
FT                   /evidence="ECO:0000305|PubMed:21515432"
FT                   /id="PRO_0000422894"
FT   DISULFID        24..44
FT                   /evidence="ECO:0000250|UniProtKB:P60301"
FT   DISULFID        38..59
FT                   /evidence="ECO:0000250|UniProtKB:P60301"
FT   DISULFID        63..74
FT                   /evidence="ECO:0000250|UniProtKB:P60301"
FT   DISULFID        75..80
FT                   /evidence="ECO:0000250|UniProtKB:P60301"
SQ   SEQUENCE   82 AA;  9070 MW;  16951C6936BED77F CRC64;
     MKTLLLTLVV VTIMCLDLGY TLVCYTNVLE PPGTLETCPD DFTCVKKWEG GGRRVTQYCS
     HACAIPASYE FVHCCQTDKC NG