ID   AP37_ORYSJ              Reviewed;         464 AA.
AC   Q7XV21;
DT   08-JUN-2016, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2004, sequence version 2.
DT   03-AUG-2022, entry version 125.
DE   RecName: Full=Aspartyl protease 37 {ECO:0000305};
DE            EC=3.4.23.- {ECO:0000305};
DE   Flags: Precursor;
GN   Name=AP37 {ECO:0000303|PubMed:23385589};
GN   OrderedLocusNames=Os04g0448500 {ECO:0000312|EMBL:BAF14837.1},
GN   LOC_Os04g37570 {ECO:0000305};
GN   ORFNames=OSJNBa0064H22.11 {ECO:0000312|EMBL:CAD40872.2};
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12447439; DOI=10.1038/nature01183;
RA   Feng Q., Zhang Y., Hao P., Wang S., Fu G., Huang Y., Li Y., Zhu J., Liu Y.,
RA   Hu X., Jia P., Zhang Y., Zhao Q., Ying K., Yu S., Tang Y., Weng Q.,
RA   Zhang L., Lu Y., Mu J., Lu Y., Zhang L.S., Yu Z., Fan D., Liu X., Lu T.,
RA   Li C., Wu Y., Sun T., Lei H., Li T., Hu H., Guan J., Wu M., Zhang R.,
RA   Zhou B., Chen Z., Chen L., Jin Z., Wang R., Yin H., Cai Z., Ren S., Lv G.,
RA   Gu W., Zhu G., Tu Y., Jia J., Zhang Y., Chen J., Kang H., Chen X., Shao C.,
RA   Sun Y., Hu Q., Zhang X., Zhang W., Wang L., Ding C., Sheng H., Gu J.,
RA   Chen S., Ni L., Zhu F., Chen W., Lan L., Lai Y., Cheng Z., Gu M., Jiang J.,
RA   Li J., Hong G., Xue Y., Han B.;
RT   "Sequence and analysis of rice chromosome 4.";
RL   Nature 420:316-320(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [5]
RP   FUNCTION.
RX   PubMed=23385589; DOI=10.1038/ncomms2396;
RA   Niu N., Liang W., Yang X., Jin W., Wilson Z.A., Hu J., Zhang D.;
RT   "EAT1 promotes tapetal cell death by regulating aspartic proteases during
RT   male reproductive development in rice.";
RL   Nat. Commun. 4:1445-1445(2013).
CC   -!- FUNCTION: Anther-specific aspartic protease involved in tapetal
CC       programmed cell death (PCD). Directly regulated by the transcription
CC       factor EAT1/DTD in anthers during tapetum PCD and degeneration.
CC       {ECO:0000269|PubMed:23385589}.
CC   -!- SIMILARITY: Belongs to the peptidase A1 family. {ECO:0000255|PROSITE-
CC       ProRule:PRU01103}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AL606448; CAD40872.2; -; Genomic_DNA.
DR   EMBL; AP008210; BAF14837.1; -; Genomic_DNA.
DR   EMBL; AP014960; BAS89430.1; -; Genomic_DNA.
DR   RefSeq; XP_015635682.1; XM_015780196.1.
DR   AlphaFoldDB; Q7XV21; -.
DR   SMR; Q7XV21; -.
DR   STRING; 4530.OS04T0448500-00; -.
DR   PaxDb; Q7XV21; -.
DR   PRIDE; Q7XV21; -.
DR   EnsemblPlants; Os04t0448500-00; Os04t0448500-00; Os04g0448500.
DR   GeneID; 4335981; -.
DR   Gramene; Os04t0448500-00; Os04t0448500-00; Os04g0448500.
DR   KEGG; osa:4335981; -.
DR   eggNOG; KOG1339; Eukaryota.
DR   HOGENOM; CLU_005738_1_3_1; -.
DR   InParanoid; Q7XV21; -.
DR   OMA; DANRYGM; -.
DR   OrthoDB; 753343at2759; -.
DR   Proteomes; UP000000763; Chromosome 4.
DR   Proteomes; UP000059680; Chromosome 4.
DR   GO; GO:0005576; C:extracellular region; IBA:GO_Central.
DR   GO; GO:0004190; F:aspartic-type endopeptidase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   GO; GO:0043067; P:regulation of programmed cell death; IMP:UniProtKB.
DR   CDD; cd05476; pepsin_A_like_plant; 1.
DR   Gene3D; 2.40.70.10; -; 2.
DR   InterPro; IPR034161; Pepsin-like_plant.
DR   InterPro; IPR033121; PEPTIDASE_A1.
DR   InterPro; IPR021109; Peptidase_aspartic_dom_sf.
DR   InterPro; IPR032799; TAXi_C.
DR   InterPro; IPR032861; TAXi_N.
DR   Pfam; PF14541; TAXi_C; 1.
DR   Pfam; PF14543; TAXi_N; 1.
DR   SUPFAM; SSF50630; SSF50630; 1.
DR   PROSITE; PS51767; PEPTIDASE_A1; 1.
PE   3: Inferred from homology;
KW   Aspartyl protease; Disulfide bond; Glycoprotein; Hydrolase; Protease;
KW   Reference proteome; Signal.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000255"
FT   CHAIN           20..464
FT                   /note="Aspartyl protease 37"
FT                   /id="PRO_5007213614"
FT   DOMAIN          89..456
FT                   /note="Peptidase A1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01103"
FT   REGION          299..319
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        107
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01103"
FT   ACT_SITE        337
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01103"
FT   CARBOHYD        41
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT   CARBOHYD        174
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT   CARBOHYD        261
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT   CARBOHYD        320
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT   DISULFID        117..123
FT                   /evidence="ECO:0000250|UniProtKB:P42210"
FT   DISULFID        376..420
FT                   /evidence="ECO:0000250|UniProtKB:P42210"
SQ   SEQUENCE   464 AA;  49354 MW;  43B1443D3B732901 CRC64;
     MNAAVLLLLL ALAALPASCA PPRSFRLELA SVDASAADAA NLTEHELLRR AIQRSRYRLA
     GIGMARGEAA SARKAVVAET PIMPAGGEYL VKLGIGTPPY KFTAAIDTAS DLIWTQCQPC
     TGCYHQVDPM FNPRVSSTYA ALPCSSDTCD ELDVHRCGHD DDESCQYTYT YSGNATTEGT
     LAVDKLVIGE DAFRGVAFGC STSSTGGAPP PQASGVVGLG RGPLSLVSQL SVRRFAYCLP
     PPASRIPGKL VLGADADAAR NATNRIAVPM RRDPRYPSYY YLNLDGLLIG DRAMSLPPTT
     TTTATATATA PAPAPTPSPN ATAVAVGDAN RYGMIIDIAS TITFLEASLY DELVNDLEVE
     IRLPRGTGSS LGLDLCFILP DGVAFDRVYV PAVALAFDGR WLRLDKARLF AEDRESGMMC
     LMVGRAEAGS VSILGNFQQQ NMQVLYNLRR GRVTFVQSPC GALR