HSF5_MOUSE
ID HSF5_MOUSE Reviewed; 624 AA.
AC Q5ND04; B2KGG8; Q3TLD8;
DT 20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-2005, sequence version 1.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=Heat shock factor protein 5;
DE Short=HSF 5;
DE AltName: Full=Heat shock transcription factor 5;
DE Short=HSTF 5;
DE AltName: Full=Protein expressed in male leptotene and zygotene spermatocytes 220;
DE Short=MLZ-220;
GN Name=Hsf5; Synonyms=Gm739;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 485-624.
RC STRAIN=C57BL/6J;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-600, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [4]
RP TISSUE SPECIFICITY.
RX PubMed=20339383; DOI=10.1038/jhg.2010.26;
RA Kogo H., Kowa-Sugiyama H., Yamada K., Bolor H., Tsutsumi M., Ohye T.,
RA Inagaki H., Taniguchi M., Toda T., Kurahashi H.;
RT "Screening of genes involved in chromosome segregation during meiosis I:
RT toward the identification of genes responsible for infertility in humans.";
RL J. Hum. Genet. 55:293-299(2010).
CC -!- FUNCTION: May act as a transcriptional factor. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in adult but not fetal testis. Not
CC expressed in ovary. {ECO:0000269|PubMed:20339383}.
CC -!- SIMILARITY: Belongs to the HSF family. {ECO:0000305}.
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DR EMBL; AL596086; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CU393486; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AK166561; BAE38854.1; -; mRNA.
DR CCDS; CCDS36269.1; -.
DR RefSeq; NP_001038992.1; NM_001045527.1.
DR AlphaFoldDB; Q5ND04; -.
DR SMR; Q5ND04; -.
DR STRING; 10090.ENSMUSP00000091488; -.
DR iPTMnet; Q5ND04; -.
DR PhosphoSitePlus; Q5ND04; -.
DR PaxDb; Q5ND04; -.
DR PeptideAtlas; Q5ND04; -.
DR PRIDE; Q5ND04; -.
DR ProteomicsDB; 267067; -.
DR Antibodypedia; 18396; 97 antibodies from 22 providers.
DR Ensembl; ENSMUST00000093956; ENSMUSP00000091488; ENSMUSG00000070345.
DR GeneID; 327992; -.
DR KEGG; mmu:327992; -.
DR UCSC; uc007kuc.1; mouse.
DR CTD; 124535; -.
DR MGI; MGI:2685585; Hsf5.
DR VEuPathDB; HostDB:ENSMUSG00000070345; -.
DR eggNOG; KOG1508; Eukaryota.
DR GeneTree; ENSGT00510000048674; -.
DR HOGENOM; CLU_037490_1_0_1; -.
DR InParanoid; Q5ND04; -.
DR OMA; HRIWQNS; -.
DR OrthoDB; 641574at2759; -.
DR PhylomeDB; Q5ND04; -.
DR TreeFam; TF330401; -.
DR BioGRID-ORCS; 327992; 2 hits in 72 CRISPR screens.
DR ChiTaRS; Hsf5; mouse.
DR PRO; PR:Q5ND04; -.
DR Proteomes; UP000000589; Chromosome 11.
DR RNAct; Q5ND04; protein.
DR Bgee; ENSMUSG00000070345; Expressed in spermatocyte and 24 other tissues.
DR Genevisible; Q5ND04; MM.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:1990837; F:sequence-specific double-stranded DNA binding; ISO:MGI.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR027736; HSF5.
DR InterPro; IPR000232; HSF_DNA-bd.
DR InterPro; IPR027725; HSF_fam.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR PANTHER; PTHR10015; PTHR10015; 1.
DR PANTHER; PTHR10015:SF278; PTHR10015:SF278; 1.
DR Pfam; PF00447; HSF_DNA-bind; 1.
DR SMART; SM00415; HSF; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Nucleus; Phosphoprotein; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..624
FT /note="Heat shock factor protein 5"
FT /id="PRO_0000333042"
FT DNA_BIND 11..228
FT /evidence="ECO:0000250"
FT REGION 52..77
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 112..138
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 186..214
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 429..461
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 572..605
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 574..591
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 600
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
SQ SEQUENCE 624 AA; 67316 MW; 26E6C5406679104B CRC64;
MEEALLSTPI NPNNFPAKLW RLVNSPRYRS IRWDGRGEGL LIDQPLFEAE LLSPPGPGAG
GAGGAGSSGG GGGSGVGAAG AEPELFKTTN FTSFIRQLNL YGFRKVVLGG PGAAGPGPGP
GAGGPAGDGP LHHFHSPHFR RDQPQLLVHL KRLTSANKAK LAAGLEVPCR PPNRFQRLLI
TSASASASAS TSPLQHQDPP PQPAGPRPEQ HGPVAVGQFH RSFRRDNLSP YSYVSTSSHN
HSAFPLKGLD RTPIPPRTWQ NSLGMHPGQV ETSPTFSDKG VPFPVLQRFP TEVTYTLQPS
ATSVHVQQGP QTMVSSSQKY SNYTPSAQYS QAYYPTAVLQ CCSPPTHMDA LSSCVTPTAS
SYAHCNYFQN PPMQSSYPVE FLPSNWPCSA TDENKKTEVN LEAVFQIVDE LHSSPKLEMV
KVEPVETQCP SSQANRGQHI LPNANSSNPS STSQASQLEP LTPVGSDITS FVVGTEQAIT
CSLPQSPEYI YTIHTAQPLE NSTMQESATI QQTHVKLKEQ LNHNPSPSSV VFVQEGLPFS
TPQVDSSIKC QTNPSENILP SEQMGFLISE MGPANKSTKD TGLSTPARYR ERRSNSQGKS
PDLHLLVDVA CKQEHFPKEE ELKE
