HSFA3_ARATH
ID HSFA3_ARATH Reviewed; 412 AA.
AC Q8GYY1; Q9LZR6;
DT 09-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT 09-JAN-2007, sequence version 2.
DT 03-AUG-2022, entry version 133.
DE RecName: Full=Heat stress transcription factor A-3;
DE Short=AtHsfA3;
DE AltName: Full=AtHsf-17;
GN Name=HSFA3; Synonyms=HSF17; OrderedLocusNames=At5g03720;
GN ORFNames=F17C15.140;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 68-412.
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [4]
RP GENE FAMILY, NOMENCLATURE, AND DOMAIN AHA.
RX PubMed=11599559; DOI=10.1379/1466-1268(2001)006<0177:aathst>2.0.co;2;
RA Nover L., Bharti K., Doering P., Mishra S.K., Ganguli A., Scharf K.-D.;
RT "Arabidopsis and the heat stress transcription factor world: how many heat
RT stress transcription factors do we need?";
RL Cell Stress Chaperones 6:177-189(2001).
RN [5]
RP FUNCTION, SUBCELLULAR LOCATION, AND INDUCTION.
RX PubMed=18261981; DOI=10.1016/j.bbrc.2008.01.134;
RA Yoshida T., Sakuma Y., Todaka D., Maruyama K., Qin F., Mizoi J.,
RA Kidokoro S., Fujita Y., Shinozaki K., Yamaguchi-Shinozaki K.;
RT "Functional analysis of an Arabidopsis heat-shock transcription factor
RT HsfA3 in the transcriptional cascade downstream of the DREB2A stress-
RT regulatory system.";
RL Biochem. Biophys. Res. Commun. 368:515-521(2008).
RN [6]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=18407058; DOI=10.1016/s1673-8527(08)60016-8;
RA Guo J., Wu J., Ji Q., Wang C., Luo L., Yuan Y., Wang Y., Wang J.;
RT "Genome-wide analysis of heat shock transcription factor families in rice
RT and Arabidopsis.";
RL J. Genet. Genomics 35:105-118(2008).
RN [7]
RP FUNCTION, AND INDUCTION.
RX PubMed=17999647; DOI=10.1111/j.1365-313x.2007.03334.x;
RA Schramm F., Larkindale J., Kiehlmann E., Ganguli A., Englich G.,
RA Vierling E., von Koskull-Doering P.;
RT "A cascade of transcription factor DREB2A and heat stress transcription
RT factor HsfA3 regulates the heat stress response of Arabidopsis.";
RL Plant J. 53:264-274(2008).
CC -!- FUNCTION: Transcriptional activator that specifically binds DNA
CC sequence 5'-AGAAnnTTCT-3' known as heat shock promoter elements (HSE).
CC Involved in heat stress response. Activated by DREB2A under heat
CC stress. {ECO:0000269|PubMed:17999647, ECO:0000269|PubMed:18261981}.
CC -!- SUBUNIT: Homotrimer. {ECO:0000250}.
CC -!- INTERACTION:
CC Q8GYY1; Q9LV52: HSFC1; NbExp=3; IntAct=EBI-15198195, EBI-4457746;
CC Q8GYY1; Q9FMC8: OFP13; NbExp=3; IntAct=EBI-15198195, EBI-15204858;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:18261981}.
CC Note=Detected under heat stress condition.
CC -!- INDUCTION: By heat stress. {ECO:0000269|PubMed:17999647,
CC ECO:0000269|PubMed:18261981}.
CC -!- DOMAIN: The hydrophobic-rich region (HR-A/B) corresponds to the
CC oligomerization domain. AHA motifs are transcriptional activator
CC elements. {ECO:0000269|PubMed:11599559}.
CC -!- PTM: Exhibits temperature-dependent phosphorylation. {ECO:0000250}.
CC -!- MISCELLANEOUS: Plants overexpressing HSFA3 show increased
CC thermotolerance.
CC -!- SIMILARITY: Belongs to the HSF family. Class A subfamily.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC41989.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=CAB82937.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AL162506; CAB82937.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED90646.1; -; Genomic_DNA.
DR EMBL; CP002688; ANM70765.1; -; Genomic_DNA.
DR EMBL; AK117318; BAC41989.1; ALT_INIT; mRNA.
DR PIR; T48399; T48399.
DR RefSeq; NP_001318473.1; NM_001342728.1.
DR RefSeq; NP_195992.2; NM_120453.4.
DR AlphaFoldDB; Q8GYY1; -.
DR SMR; Q8GYY1; -.
DR BioGRID; 17025; 18.
DR IntAct; Q8GYY1; 15.
DR STRING; 3702.AT5G03720.1; -.
DR PaxDb; Q8GYY1; -.
DR PRIDE; Q8GYY1; -.
DR ProteomicsDB; 232110; -.
DR EnsemblPlants; AT5G03720.1; AT5G03720.1; AT5G03720.
DR EnsemblPlants; AT5G03720.2; AT5G03720.2; AT5G03720.
DR GeneID; 831749; -.
DR Gramene; AT5G03720.1; AT5G03720.1; AT5G03720.
DR Gramene; AT5G03720.2; AT5G03720.2; AT5G03720.
DR KEGG; ath:AT5G03720; -.
DR Araport; AT5G03720; -.
DR TAIR; locus:2144603; AT5G03720.
DR eggNOG; KOG0627; Eukaryota.
DR HOGENOM; CLU_030308_9_0_1; -.
DR InParanoid; Q8GYY1; -.
DR OMA; KQEETTW; -.
DR OrthoDB; 1154048at2759; -.
DR PhylomeDB; Q8GYY1; -.
DR PRO; PR:Q8GYY1; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q8GYY1; baseline and differential.
DR Genevisible; Q8GYY1; AT.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0003677; F:DNA binding; IDA:TAIR.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0034605; P:cellular response to heat; IBA:GO_Central.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IDA:TAIR.
DR GO; GO:0009408; P:response to heat; IEP:TAIR.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR000232; HSF_DNA-bd.
DR InterPro; IPR027725; HSF_fam.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR PANTHER; PTHR10015; PTHR10015; 1.
DR Pfam; PF00447; HSF_DNA-bind; 1.
DR PRINTS; PR00056; HSFDOMAIN.
DR SMART; SM00415; HSF; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
DR PROSITE; PS00434; HSF_DOMAIN; 1.
PE 1: Evidence at protein level;
KW Activator; Coiled coil; DNA-binding; Nucleus; Phosphoprotein;
KW Reference proteome; Repeat; Stress response; Transcription;
KW Transcription regulation.
FT CHAIN 1..412
FT /note="Heat stress transcription factor A-3"
FT /id="PRO_0000270803"
FT DNA_BIND 53..147
FT /evidence="ECO:0000250"
FT REGION 144..170
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 159..225
FT /note="Hydrophobic repeat HR-A/B"
FT COILED 166..224
FT /evidence="ECO:0000255"
FT MOTIF 238..254
FT /note="Bipartite nuclear localization signal"
FT /evidence="ECO:0000255"
FT MOTIF 277..286
FT /note="AHA1"
FT MOTIF 381..390
FT /note="AHA2"
FT COMPBIAS 145..167
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 412 AA; 46458 MW; 5900A13F8AC147BD CRC64;
MSPKKDAVSK PTPISVPVSR RSDIPGSLYV DTDMGFSGSP LPMPLDILQG NPIPPFLSKT
FDLVDDPTLD PVISWGLTGA SFVVWDPLEF ARIILPRNFK HNNFSSFVRQ LNTYGFRKID
TDKWEFANEA FLRGKKHLLK NIHRRRSPQS NQTCCSSTSQ SQGSPTEVGG EIEKLRKERR
ALMEEMVELQ QQSRGTARHV DTVNQRLKAA EQRQKQLLSF LAKLFQNRGF LERLKNFKGK
EKGGALGLEK ARKKFIKHHQ QPQDSPTGGE VVKYEADDWE RLLMYDEETE NTKGLGGMTS
SDPKGKNLMY PSEEEMSKPD YLMSFPSPEG LIKQEETTWS MGFDTTIPSF SNTDAWGNTM
DYNDVSEFGF AAETTSDGLP DVCWEQFAAG ITETGFNWPT GDDDDNTPMN DP
