ID   HSFA9_ARATH             Reviewed;         331 AA.
AC   Q9LVW2;
DT   09-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   25-MAY-2022, entry version 144.
DE   RecName: Full=Heat stress transcription factor A-9;
DE            Short=AtHsfA9;
DE   AltName: Full=AtHsf-21;
GN   Name=HSFA9; Synonyms=HSF21; OrderedLocusNames=At5g54070; ORFNames=MJP23.4;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10718197; DOI=10.1093/dnares/7.1.31;
RA   Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H.,
RA   Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence
RT   features of the regions of 3,076,755 bp covered by sixty P1 and TAC
RT   clones.";
RL   DNA Res. 7:31-63(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA   Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA   Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA   Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA   Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA   Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA   Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA   Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA   Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA   Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA   Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA   Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [4]
RP   GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=11599559; DOI=10.1379/1466-1268(2001)006<0177:aathst>2.0.co;2;
RA   Nover L., Bharti K., Doering P., Mishra S.K., Ganguli A., Scharf K.-D.;
RT   "Arabidopsis and the heat stress transcription factor world: how many heat
RT   stress transcription factors do we need?";
RL   Cell Stress Chaperones 6:177-189(2001).
RN   [5]
RP   FUNCTION, AND DEVELOPMENTAL STAGE.
RX   PubMed=17220197; DOI=10.1105/tpc.106.048165;
RA   Kotak S., Vierling E., Baeumlein H., von Koskull-Doering P.;
RT   "A novel transcriptional cascade regulating expression of heat stress
RT   proteins during seed development of Arabidopsis.";
RL   Plant Cell 19:182-195(2007).
RN   [6]
RP   GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=18407058; DOI=10.1016/s1673-8527(08)60016-8;
RA   Guo J., Wu J., Ji Q., Wang C., Luo L., Yuan Y., Wang Y., Wang J.;
RT   "Genome-wide analysis of heat shock transcription factor families in rice
RT   and Arabidopsis.";
RL   J. Genet. Genomics 35:105-118(2008).
CC   -!- FUNCTION: Seed-specific transcriptional regulator that specifically
CC       binds DNA sequence 5'-AGAAnnTTCT-3' known as heat shock promoter
CC       elements (HSE). Seems to be specialized for the developmental
CC       expression of heat shock protein (HSP) genes during seed maturation.
CC       Activated by ABI3. {ECO:0000269|PubMed:17220197}.
CC   -!- SUBUNIT: Homotrimer. {ECO:0000250}.
CC   -!- INTERACTION:
CC       Q9LVW2; Q9LQT8: GAI; NbExp=3; IntAct=EBI-15200782, EBI-963606;
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- DEVELOPMENTAL STAGE: Starts to be expressed in seeds from 18 days after
CC       pollination (DAP) to reach the highest expression in dry seeds.
CC       Expression is strongly reduced after only 2 hours of seed imbibition,
CC       declines drastically after 6 hours, but can be detected at a very low
CC       level even after 10 hours. {ECO:0000269|PubMed:17220197}.
CC   -!- DOMAIN: The hydrophobic-rich region (HR-A/B) corresponds to the
CC       oligomerization domain.
CC   -!- PTM: Exhibits temperature-dependent phosphorylation. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the HSF family. Class A subfamily.
CC       {ECO:0000305}.
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DR   EMBL; AB018115; BAA97129.1; -; Genomic_DNA.
DR   EMBL; CP002688; AED96446.1; -; Genomic_DNA.
DR   EMBL; AY065226; AAL38702.1; -; mRNA.
DR   EMBL; AY096552; AAM20202.1; -; mRNA.
DR   RefSeq; NP_200218.1; NM_124786.4.
DR   AlphaFoldDB; Q9LVW2; -.
DR   SMR; Q9LVW2; -.
DR   BioGRID; 20737; 10.
DR   IntAct; Q9LVW2; 9.
DR   STRING; 3702.AT5G54070.1; -.
DR   PaxDb; Q9LVW2; -.
DR   PRIDE; Q9LVW2; -.
DR   ProteomicsDB; 230153; -.
DR   EnsemblPlants; AT5G54070.1; AT5G54070.1; AT5G54070.
DR   GeneID; 835493; -.
DR   Gramene; AT5G54070.1; AT5G54070.1; AT5G54070.
DR   KEGG; ath:AT5G54070; -.
DR   Araport; AT5G54070; -.
DR   TAIR; locus:2166562; AT5G54070.
DR   eggNOG; KOG0627; Eukaryota.
DR   HOGENOM; CLU_840322_0_0_1; -.
DR   InParanoid; Q9LVW2; -.
DR   OMA; HMLSFFA; -.
DR   OrthoDB; 1154048at2759; -.
DR   PhylomeDB; Q9LVW2; -.
DR   PRO; PR:Q9LVW2; -.
DR   Proteomes; UP000006548; Chromosome 5.
DR   ExpressionAtlas; Q9LVW2; baseline and differential.
DR   Genevisible; Q9LVW2; AT.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR   GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR   GO; GO:0034605; P:cellular response to heat; IBA:GO_Central.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR000232; HSF_DNA-bd.
DR   InterPro; IPR027725; HSF_fam.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   PANTHER; PTHR10015; PTHR10015; 1.
DR   Pfam; PF00447; HSF_DNA-bind; 1.
DR   PRINTS; PR00056; HSFDOMAIN.
DR   SMART; SM00415; HSF; 1.
DR   SUPFAM; SSF46785; SSF46785; 1.
DR   PROSITE; PS00434; HSF_DOMAIN; 1.
PE   1: Evidence at protein level;
KW   DNA-binding; Nucleus; Phosphoprotein; Reference proteome; Stress response;
KW   Transcription; Transcription regulation.
FT   CHAIN           1..331
FT                   /note="Heat stress transcription factor A-9"
FT                   /id="PRO_0000270811"
FT   DNA_BIND        69..163
FT                   /evidence="ECO:0000250"
FT   REGION          14..39
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          175..234
FT                   /note="Hydrophobic repeat HR-A/B"
FT   MOTIF           245..265
FT                   /note="Bipartite nuclear localization signal"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   331 AA;  38146 MW;  19EB91073A088F59 CRC64;
     MTAIPNVVDI ESSSSSLCQE TATETVTVER GSSDSSSKPD DVVLLIKEEE DDAVNLSLGF
     WKLHEIGLIT PFLRKTFEIV DDKVTDPVVS WSPTRKSFII WDSYEFSENL LPKYFKHKNF
     SSFIRQLNSY GFKKVDSDRW EFANEGFQGG KKHLLKNIKR RSKNTKCCNK EASTTTTETE
     VESLKEEQSP MRLEMLKLKQ QQEESQHQMV TVQEKIHGVD TEQQHMLSFF AKLAKDQRFV
     ERLVKKRKMK IQRELEAAEF VKKLKLLQDQ ETQKNLLDVE REFMAMAATE HNPEPDILVN
     NQSGNTRCQL NSEDLLVDGG SMDVNGRIEI E