HSFY1_HUMAN
ID HSFY1_HUMAN Reviewed; 401 AA.
AC Q96LI6; Q17RC0; Q7Z4L8; Q9BZA2; Q9BZA3;
DT 17-JAN-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 179.
DE RecName: Full=Heat shock transcription factor, Y-linked;
DE AltName: Full=Heat shock transcription factor 2-like protein;
DE Short=HSF2-like;
GN Name=HSFY1; Synonyms=HSF2L, HSFY;
GN and
GN Name=HSFY2; Synonyms=HSF2L, HSFY;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3).
RC TISSUE=Testis;
RA Salata E., Tessari A., Ferlin A., Bartoloni L., Slongo M., Foresta C.;
RT "Characterization of HSFY, a novel AZFb gene on the Y chromosome with a
RT possible role in human spermatogenesis.";
RL Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), AND TISSUE SPECIFICITY.
RX PubMed=12815422; DOI=10.1038/nature01722;
RA Skaletsky H., Kuroda-Kawaguchi T., Minx P.J., Cordum H.S., Hillier L.W.,
RA Brown L.G., Repping S., Pyntikova T., Ali J., Bieri T., Chinwalla A.,
RA Delehaunty A., Delehaunty K., Du H., Fewell G., Fulton L., Fulton R.,
RA Graves T.A., Hou S.-F., Latrielle P., Leonard S., Mardis E., Maupin R.,
RA McPherson J., Miner T., Nash W., Nguyen C., Ozersky P., Pepin K., Rock S.,
RA Rohlfing T., Scott K., Schultz B., Strong C., Tin-Wollam A., Yang S.-P.,
RA Waterston R.H., Wilson R.K., Rozen S., Page D.C.;
RT "The male-specific region of the human Y chromosome is a mosaic of discrete
RT sequence classes.";
RL Nature 423:825-837(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Testis;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP TISSUE SPECIFICITY, AND SUBCELLULAR LOCATION.
RX PubMed=15044259; DOI=10.1095/biolreprod.103.023580;
RA Shinka T., Sato Y., Chen G., Naroda T., Kinoshita K., Unemi Y., Tsuji K.,
RA Toida K., Iwamoto T., Nakahori Y.;
RT "Molecular characterization of heat shock-like factor encoded on the human
RT Y chromosome, and implications for male infertility.";
RL Biol. Reprod. 71:297-306(2004).
CC -!- INTERACTION:
CC Q96LI6; P54253: ATXN1; NbExp=3; IntAct=EBI-3957665, EBI-930964;
CC Q96LI6; B4DE54: BANP; NbExp=3; IntAct=EBI-3957665, EBI-16429313;
CC Q96LI6; Q8N9N5-7: BANP; NbExp=3; IntAct=EBI-3957665, EBI-16429296;
CC Q96LI6; Q9NP55: BPIFA1; NbExp=3; IntAct=EBI-3957665, EBI-953896;
CC Q96LI6; O75909-2: CCNK; NbExp=3; IntAct=EBI-3957665, EBI-12010594;
CC Q96LI6; Q15038: DAZAP2; NbExp=3; IntAct=EBI-3957665, EBI-724310;
CC Q96LI6; Q86UW9: DTX2; NbExp=3; IntAct=EBI-3957665, EBI-740376;
CC Q96LI6; O75603: GCM2; NbExp=12; IntAct=EBI-3957665, EBI-10188645;
CC Q96LI6; O14964: HGS; NbExp=3; IntAct=EBI-3957665, EBI-740220;
CC Q96LI6; P52597: HNRNPF; NbExp=3; IntAct=EBI-3957665, EBI-352986;
CC Q96LI6; Q8WXH2: JPH3; NbExp=3; IntAct=EBI-3957665, EBI-1055254;
CC Q96LI6; Q13952-2: NFYC; NbExp=3; IntAct=EBI-3957665, EBI-11956831;
CC Q96LI6; Q6GQQ9: OTUD7B; NbExp=3; IntAct=EBI-3957665, EBI-527784;
CC Q96LI6; P16284: PECAM1; NbExp=3; IntAct=EBI-3957665, EBI-716404;
CC Q96LI6; Q2TAL8: QRICH1; NbExp=3; IntAct=EBI-3957665, EBI-2798044;
CC Q96LI6; B7ZLP7: RBM47; NbExp=3; IntAct=EBI-3957665, EBI-10176148;
CC Q96LI6; O60248: SOX15; NbExp=3; IntAct=EBI-3957665, EBI-5452954;
CC Q96LI6; Q7L1I2: SV2B; NbExp=3; IntAct=EBI-3957665, EBI-10290607;
CC Q96LI6; O95947: TBX6; NbExp=7; IntAct=EBI-3957665, EBI-2824328;
CC Q96LI6; Q96NM4-3: TOX2; NbExp=3; IntAct=EBI-3957665, EBI-12815137;
CC Q96LI6; P61086: UBE2K; NbExp=3; IntAct=EBI-3957665, EBI-473850;
CC Q96LI6; P08670: VIM; NbExp=3; IntAct=EBI-3957665, EBI-353844;
CC Q96LI6-3; P31749: AKT1; NbExp=3; IntAct=EBI-25830912, EBI-296087;
CC Q96LI6-3; P23560-2: BDNF; NbExp=3; IntAct=EBI-25830912, EBI-12275524;
CC Q96LI6-3; P02511: CRYAB; NbExp=3; IntAct=EBI-25830912, EBI-739060;
CC Q96LI6-3; P07900: HSP90AA1; NbExp=3; IntAct=EBI-25830912, EBI-296047;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:15044259}. Cytoplasm
CC {ECO:0000269|PubMed:15044259}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q96LI6-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q96LI6-2; Sequence=VSP_009179, VSP_009181;
CC Name=3;
CC IsoId=Q96LI6-3; Sequence=VSP_009180, VSP_009182;
CC -!- TISSUE SPECIFICITY: Testis-specific. Present in Sertoli cells and
CC spermatogenic cells (at protein level). {ECO:0000269|PubMed:12815422,
CC ECO:0000269|PubMed:15044259}.
CC -!- SIMILARITY: Belongs to the HSF family. {ECO:0000305}.
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DR EMBL; AJ566404; CAD98806.1; -; mRNA.
DR EMBL; AF332226; AAK13478.1; -; mRNA.
DR EMBL; AF332227; AAK13479.1; -; mRNA.
DR EMBL; AK058182; BAB71706.1; -; mRNA.
DR EMBL; BC036567; AAH36567.1; -; mRNA.
DR EMBL; BC055414; AAH55414.1; -; mRNA.
DR EMBL; BC117380; AAI17381.1; -; mRNA.
DR EMBL; BC117382; AAI17383.1; -; mRNA.
DR CCDS; CCDS14790.1; -. [Q96LI6-2]
DR CCDS; CCDS14791.1; -. [Q96LI6-1]
DR CCDS; CCDS35475.1; -. [Q96LI6-1]
DR CCDS; CCDS35476.1; -. [Q96LI6-2]
DR RefSeq; NP_001001877.1; NM_001001877.1. [Q96LI6-2]
DR RefSeq; NP_149099.2; NM_033108.2. [Q96LI6-1]
DR RefSeq; NP_689797.1; NM_152584.1. [Q96LI6-2]
DR RefSeq; NP_714927.1; NM_153716.1. [Q96LI6-1]
DR RefSeq; XP_016885520.1; XM_017030031.1. [Q96LI6-3]
DR AlphaFoldDB; Q96LI6; -.
DR BioGRID; 124576; 40.
DR BioGRID; 127733; 10.
DR IntAct; Q96LI6; 45.
DR MINT; Q96LI6; -.
DR iPTMnet; Q96LI6; -.
DR PhosphoSitePlus; Q96LI6; -.
DR BioMuta; HSFY1; -.
DR DMDM; 27805481; -.
DR MassIVE; Q96LI6; -.
DR PaxDb; Q96LI6; -.
DR PeptideAtlas; Q96LI6; -.
DR PRIDE; Q96LI6; -.
DR Antibodypedia; 35123; 36 antibodies from 11 providers.
DR Antibodypedia; 5617; 117 antibodies from 18 providers.
DR DNASU; 86614; -.
DR Ensembl; ENST00000304790.3; ENSP00000306549.3; ENSG00000169953.12. [Q96LI6-1]
DR Ensembl; ENST00000307393.3; ENSP00000303599.2; ENSG00000172468.14. [Q96LI6-1]
DR Ensembl; ENST00000309834.8; ENSP00000311166.4; ENSG00000172468.14. [Q96LI6-2]
DR Ensembl; ENST00000338876.8; ENSP00000342302.4; ENSG00000172468.14. [Q96LI6-3]
DR Ensembl; ENST00000344884.4; ENSP00000340971.4; ENSG00000169953.12. [Q96LI6-2]
DR Ensembl; ENST00000382852.1; ENSP00000372303.1; ENSG00000169953.12. [Q96LI6-3]
DR Ensembl; ENST00000382856.2; ENSP00000372307.2; ENSG00000172468.14. [Q96LI6-3]
DR GeneID; 159119; -.
DR GeneID; 86614; -.
DR KEGG; hsa:159119; -.
DR KEGG; hsa:86614; -.
DR MANE-Select; ENST00000304790.3; ENSP00000306549.3; NM_153716.2; NP_714927.1.
DR MANE-Select; ENST00000307393.3; ENSP00000303599.2; NM_033108.3; NP_149099.2.
DR UCSC; uc004ftp.3; human. [Q96LI6-1]
DR CTD; 159119; -.
DR CTD; 86614; -.
DR DisGeNET; 159119; -.
DR DisGeNET; 86614; -.
DR GeneCards; HSFY1; -.
DR GeneCards; HSFY2; -.
DR GeneReviews; HSFY1; -.
DR HGNC; HGNC:18568; HSFY1.
DR HGNC; HGNC:23950; HSFY2.
DR HPA; ENSG00000169953; Tissue enriched (testis).
DR HPA; ENSG00000172468; Tissue enriched (testis).
DR MalaCards; HSFY1; -.
DR MIM; 400029; gene.
DR neXtProt; NX_Q96LI6; -.
DR OpenTargets; ENSG00000169953; -.
DR PharmGKB; PA38580; -.
DR VEuPathDB; HostDB:ENSG00000169953; -.
DR VEuPathDB; HostDB:ENSG00000172468; -.
DR GeneTree; ENSGT00940000157452; -.
DR HOGENOM; CLU_053156_1_0_1; -.
DR InParanoid; Q96LI6; -.
DR OMA; LLPFCNP; -.
DR OrthoDB; 843026at2759; -.
DR PhylomeDB; Q96LI6; -.
DR TreeFam; TF330401; -.
DR PathwayCommons; Q96LI6; -.
DR SignaLink; Q96LI6; -.
DR BioGRID-ORCS; 159119; 8 hits in 609 CRISPR screens.
DR BioGRID-ORCS; 86614; 15 hits in 605 CRISPR screens.
DR ChiTaRS; HSFY1; human.
DR ChiTaRS; HSFY2; human.
DR Pharos; Q96LI6; Tdark.
DR PRO; PR:Q96LI6; -.
DR Proteomes; UP000005640; Chromosome Y.
DR RNAct; Q96LI6; protein.
DR Bgee; ENSG00000169953; Expressed in testis and 43 other tissues.
DR Genevisible; Q96LI6; HS.
DR GO; GO:0000785; C:chromatin; ISA:NTNU_SB.
DR GO; GO:0005829; C:cytosol; IDA:HPA.
DR GO; GO:0005654; C:nucleoplasm; IDA:HPA.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IBA:GO_Central.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; ISA:NTNU_SB.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:1990837; F:sequence-specific double-stranded DNA binding; IDA:ARUK-UCL.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR000232; HSF_DNA-bd.
DR InterPro; IPR027725; HSF_fam.
DR InterPro; IPR027730; HSFY.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR PANTHER; PTHR10015; PTHR10015; 1.
DR PANTHER; PTHR10015:SF336; PTHR10015:SF336; 1.
DR Pfam; PF00447; HSF_DNA-bind; 1.
DR SMART; SM00415; HSF; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasm; DNA-binding; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..401
FT /note="Heat shock transcription factor, Y-linked"
FT /id="PRO_0000124573"
FT DNA_BIND 76..194
FT /evidence="ECO:0000250|UniProtKB:P10961"
FT REGION 1..30
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..27
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 172..203
FT /note="LKFYYNPNFKRGYPQLLVRVKRRIGVKNASPI -> IRFTKMKLSRSSTYEN
FT RYLCCNLHLKDESNYS (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:12815422"
FT /id="VSP_009179"
FT VAR_SEQ 173..214
FT /note="KFYYNPNFKRGYPQLLVRVKRRIGVKNASPISTLFNEDFNKK -> HVFVFH
FT HSLFGATFGEHHFQPCPLPSGNKAPISTWSGDSWLL (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:15489334, ECO:0000303|Ref.1"
FT /id="VSP_009180"
FT VAR_SEQ 204..401
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:12815422"
FT /id="VSP_009181"
FT VAR_SEQ 215..401
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:15489334, ECO:0000303|Ref.1"
FT /id="VSP_009182"
FT CONFLICT 94..99
FT /note="KSISWD -> SLFSWE (in Ref. 2; AAK13479)"
FT /evidence="ECO:0000305"
FT CONFLICT 164
FT /note="K -> I (in Ref. 2; AAK13479)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 401 AA; 45107 MW; 1990C4D91E0BB277 CRC64;
MAHVSSETQD VSPKDELTAS EASTRSPLCE HTFPGDSDLR SMIEEHAFQV LSQGSLLESP
SYTVCVSEPD KDDDFLSLNF PRKLWKIVES DQFKSISWDE NGTCIVINEE LFKKEILETK
APYRIFQTDA IKSFVRQLNL YGFSKIQQNF QRSAFLATFL SEEKESSVLS KLKFYYNPNF
KRGYPQLLVR VKRRIGVKNA SPISTLFNED FNKKHFRAGA NMENHNSALA AEASEESLFS
ASKNLNMPLT RESSVRQIIA NSSVPIRSGF PPPSPSTSVG PSEQIATDQH AILNQLTTIH
MHSHSTYMQA RGHIVNFITT TTSQYHIISP LQNGYFGLTV EPSAVPTRYP LVSVNEAPYR
NMLPAGNPWL QMPTIADRSA APHSRLALQP SPLDKYHPNY N
