AP3B_ASHGO
ID AP3B_ASHGO Reviewed; 781 AA.
AC Q759E2;
DT 21-MAR-2006, integrated into UniProtKB/Swiss-Prot.
DT 09-JAN-2013, sequence version 2.
DT 25-MAY-2022, entry version 114.
DE RecName: Full=AP-3 complex subunit beta;
DE AltName: Full=Adaptor-related protein complex 3 subunit beta;
DE AltName: Full=Beta-3-adaptin;
DE AltName: Full=Clathrin assembly protein complex 3 beta large chain;
DE AltName: Full=Clathrin assembly protein large beta chain;
GN Name=APL6; OrderedLocusNames=ADR335C;
OS Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)
OS (Yeast) (Eremothecium gossypii).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Eremothecium.
OX NCBI_TaxID=284811;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX PubMed=15001715; DOI=10.1126/science.1095781;
RA Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S.,
RA Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A.,
RA Gaffney T.D., Philippsen P.;
RT "The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces
RT cerevisiae genome.";
RL Science 304:304-307(2004).
RN [2]
RP GENOME REANNOTATION, AND SEQUENCE REVISION TO 345.
RC STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX PubMed=23749448; DOI=10.1534/g3.112.002881;
RA Dietrich F.S., Voegeli S., Kuo S., Philippsen P.;
RT "Genomes of Ashbya fungi isolated from insects reveal four mating-type
RT loci, numerous translocations, lack of transposons, and distinct gene
RT duplications.";
RL G3 (Bethesda) 3:1225-1239(2013).
CC -!- FUNCTION: Part of the AP-3 complex, an adaptor-related complex which is
CC not clathrin-associated. The complex is associated with the Golgi
CC region as well as more peripheral structures. It facilitates the
CC budding of vesicles from the Golgi membrane and may be directly
CC involved in trafficking to the vacuole (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Adaptor protein complex 3 (AP-3) is a heterotetramer composed
CC of 2 large adaptins (APL5 and APL6), a medium adaptin (APM3) and a
CC small adaptin (APS3). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus {ECO:0000250|UniProtKB:P46682}.
CC Cytoplasmic vesicle, clathrin-coated vesicle membrane
CC {ECO:0000250|UniProtKB:P46682}; Peripheral membrane protein
CC {ECO:0000250|UniProtKB:P46682}; Cytoplasmic side
CC {ECO:0000250|UniProtKB:P46682}. Note=Component of the coat surrounding
CC the cytoplasmic face of coated vesicles located at the Golgi complex.
CC {ECO:0000250|UniProtKB:P46682}.
CC -!- SIMILARITY: Belongs to the adaptor complexes large subunit family.
CC {ECO:0000305}.
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DR EMBL; AE016817; AAS52255.2; -; Genomic_DNA.
DR RefSeq; NP_984431.2; NM_209784.2.
DR AlphaFoldDB; Q759E2; -.
DR SMR; Q759E2; -.
DR STRING; 33169.AAS52255; -.
DR EnsemblFungi; AAS52255; AAS52255; AGOS_ADR335C.
DR GeneID; 4620597; -.
DR KEGG; ago:AGOS_ADR335C; -.
DR eggNOG; KOG1060; Eukaryota.
DR HOGENOM; CLU_006320_3_2_1; -.
DR InParanoid; Q759E2; -.
DR OMA; IGRCAQS; -.
DR Proteomes; UP000000591; Chromosome IV.
DR GO; GO:0030123; C:AP-3 adaptor complex; IEA:EnsemblFungi.
DR GO; GO:0030665; C:clathrin-coated vesicle membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005794; C:Golgi apparatus; IEA:UniProtKB-SubCell.
DR GO; GO:0006896; P:Golgi to vacuole transport; IEA:EnsemblFungi.
DR GO; GO:0006623; P:protein targeting to vacuole; IEA:EnsemblFungi.
DR GO; GO:0016192; P:vesicle-mediated transport; IBA:GO_Central.
DR Gene3D; 1.25.10.10; -; 1.
DR InterPro; IPR026740; AP3_beta.
DR InterPro; IPR026739; AP_beta.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR002553; Clathrin/coatomer_adapt-like_N.
DR PANTHER; PTHR11134; PTHR11134; 1.
DR PANTHER; PTHR11134:SF1; PTHR11134:SF1; 1.
DR Pfam; PF01602; Adaptin_N; 1.
DR SUPFAM; SSF48371; SSF48371; 1.
PE 3: Inferred from homology;
KW Cytoplasmic vesicle; Golgi apparatus; Membrane; Protein transport;
KW Reference proteome; Repeat; Transport.
FT CHAIN 1..781
FT /note="AP-3 complex subunit beta"
FT /id="PRO_0000227677"
FT REPEAT 113..151
FT /note="HEAT 1"
FT REPEAT 153..186
FT /note="HEAT 2"
FT REPEAT 187..224
FT /note="HEAT 3"
FT REPEAT 294..332
FT /note="HEAT 4"
FT REPEAT 521..559
FT /note="HEAT 5"
FT REGION 694..713
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 731..781
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 738..760
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 763..781
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 781 AA; 87649 MW; C257C15EF63EF0FD CRC64;
MAESMQRIAS AFESARDLTL EAAAVASSRL GESSYFHYSK SIDSESLPGL LNSRYTREVR
DGMKRVMSLM GSGDSSIELE THFADIVKNI GSDDVKVRRM VSVYLLRYAE TNPNLALLSI
NSIQRSLSDS NPDVRALALK TLSDINIASL YPIILHSLKK TVIDSSEVVR CQVAMTLLKL
FKEQGISIKD DVMPMLKSLL ADSEPSVVSA ALLLFQKAFA QELQLLHGHY RRYCSILNQL
TENAQAIMID IFIAYAKEYL PRPMVRDTSS DAEAIQLPDS FNQIPFPVYD VEYDHDLNLF
LSSLKKLLHS PNAMVIVAVS KAFYQLSSPK TFKDSGIVDS LLRLTVSSYI CSEIKELVLQ
SILVYCCSDP SLFRSHYKRF FLMPSDTENI SIFKLKILSI LVSDSNCKHI VNELKFVAGT
EQSASILVEV SNTLSVCAQI SSKWSSQIIS WLLDRISSNT PIDKEVTASQ INVLRSLIQK
DPIKHIATVV KLSKMLSNHD LLPSAKAPII WLLGEYVQVE PRICPDVLRR LLPQFSKEHA
HVRLQILNLA AKLLSHDVDS YSGDMEYDIG TSRIGQMFEA ALQLAKFDDE YDVRDRARML
ASIFEQKRYE IATLLLQAPK PYPMASLNYC RDSFNLSALN LYPDMENYYE LLPWNADPQA
SDRTPCEVKD YSKLKNSFSS SSYFGRNEVE FTNKPKRSAS VSSVPSNTFT SSHGKKYQLQ
SLDEFFSDVS ARQSVPKAKR VIIEESTSEE TDHTDDESGS SSGDESTESS YVSSSEEETT
E
