AP3D_SCHPO
ID AP3D_SCHPO Reviewed; 825 AA.
AC Q9UTL8;
DT 21-MAR-2006, integrated into UniProtKB/Swiss-Prot.
DT 13-JUN-2012, sequence version 2.
DT 25-MAY-2022, entry version 132.
DE RecName: Full=AP-3 complex subunit delta;
DE AltName: Full=Adaptor-related protein complex 3 subunit delta;
DE AltName: Full=Delta-adaptin 3;
DE Short=Delta-adaptin;
GN Name=apl5; ORFNames=SPAC144.06;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP REVISION OF GENE MODEL.
RX PubMed=21511999; DOI=10.1126/science.1203357;
RA Rhind N., Chen Z., Yassour M., Thompson D.A., Haas B.J., Habib N.,
RA Wapinski I., Roy S., Lin M.F., Heiman D.I., Young S.K., Furuya K., Guo Y.,
RA Pidoux A., Chen H.M., Robbertse B., Goldberg J.M., Aoki K., Bayne E.H.,
RA Berlin A.M., Desjardins C.A., Dobbs E., Dukaj L., Fan L., FitzGerald M.G.,
RA French C., Gujja S., Hansen K., Keifenheim D., Levin J.Z., Mosher R.A.,
RA Mueller C.A., Pfiffner J., Priest M., Russ C., Smialowska A., Swoboda P.,
RA Sykes S.M., Vaughn M., Vengrova S., Yoder R., Zeng Q., Allshire R.,
RA Baulcombe D., Birren B.W., Brown W., Ekwall K., Kellis M., Leatherwood J.,
RA Levin H., Margalit H., Martienssen R., Nieduszynski C.A., Spatafora J.W.,
RA Friedman N., Dalgaard J.Z., Baumann P., Niki H., Regev A., Nusbaum C.;
RT "Comparative functional genomics of the fission yeasts.";
RL Science 332:930-936(2011).
CC -!- FUNCTION: Part of the AP-3 complex, an adaptor-related complex which is
CC not clathrin-associated. The complex is associated with the Golgi
CC region as well as more peripheral structures. It facilitates the
CC budding of vesicles from the Golgi membrane and may be directly
CC involved in trafficking to the vacuole (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Adaptor protein complex 3 (AP-3) is a heterotetramer composed
CC of 2 large adaptins (apl5 and apl6), a medium adaptin (apm3) and a
CC small adaptin (aps3). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus {ECO:0000250|UniProtKB:Q08951}.
CC Cytoplasmic vesicle, clathrin-coated vesicle membrane
CC {ECO:0000250|UniProtKB:Q08951}; Peripheral membrane protein
CC {ECO:0000250|UniProtKB:Q08951}; Cytoplasmic side
CC {ECO:0000250|UniProtKB:Q08951}. Note=Component of the coat surrounding
CC the cytoplasmic face of coated vesicles located at the Golgi complex.
CC {ECO:0000250|UniProtKB:Q08951}.
CC -!- SIMILARITY: Belongs to the adaptor complexes large subunit family.
CC {ECO:0000305}.
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DR EMBL; CU329670; CAB59686.2; -; Genomic_DNA.
DR PIR; T37673; T37673.
DR RefSeq; NP_594667.2; NM_001020096.2.
DR AlphaFoldDB; Q9UTL8; -.
DR SMR; Q9UTL8; -.
DR BioGRID; 279303; 32.
DR STRING; 4896.SPAC144.06.1; -.
DR iPTMnet; Q9UTL8; -.
DR MaxQB; Q9UTL8; -.
DR PaxDb; Q9UTL8; -.
DR EnsemblFungi; SPAC144.06.1; SPAC144.06.1:pep; SPAC144.06.
DR GeneID; 2542857; -.
DR KEGG; spo:SPAC144.06; -.
DR PomBase; SPAC144.06; apl5.
DR VEuPathDB; FungiDB:SPAC144.06; -.
DR eggNOG; KOG1059; Eukaryota.
DR HOGENOM; CLU_001908_1_0_1; -.
DR InParanoid; Q9UTL8; -.
DR OMA; FGHDMSW; -.
DR PRO; PR:Q9UTL8; -.
DR Proteomes; UP000002485; Chromosome I.
DR GO; GO:0030123; C:AP-3 adaptor complex; IBA:GO_Central.
DR GO; GO:0030665; C:clathrin-coated vesicle membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0010008; C:endosome membrane; IBA:GO_Central.
DR GO; GO:0005794; C:Golgi apparatus; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; HDA:PomBase.
DR GO; GO:0006896; P:Golgi to vacuole transport; ISO:PomBase.
DR GO; GO:0006623; P:protein targeting to vacuole; IBA:GO_Central.
DR Gene3D; 1.25.10.10; -; 1.
DR InterPro; IPR017105; AP3_complex_dsu.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR002553; Clathrin/coatomer_adapt-like_N.
DR PANTHER; PTHR22781; PTHR22781; 1.
DR Pfam; PF01602; Adaptin_N; 1.
DR PIRSF; PIRSF037092; AP3_complex_delta; 1.
DR SUPFAM; SSF48371; SSF48371; 1.
PE 3: Inferred from homology;
KW Cytoplasmic vesicle; Golgi apparatus; Membrane; Protein transport;
KW Reference proteome; Repeat; Transport.
FT CHAIN 1..825
FT /note="AP-3 complex subunit delta"
FT /id="PRO_0000227680"
FT REPEAT 131..168
FT /note="HEAT 1"
FT REPEAT 169..205
FT /note="HEAT 2"
FT REPEAT 207..243
FT /note="HEAT 3"
FT REPEAT 244..281
FT /note="HEAT 4"
FT REPEAT 285..323
FT /note="HEAT 5"
FT REPEAT 324..360
FT /note="HEAT 6"
FT REPEAT 363..400
FT /note="HEAT 7"
FT REPEAT 469..513
FT /note="HEAT 8"
FT REPEAT 515..547
FT /note="HEAT 9"
FT REPEAT 548..584
FT /note="HEAT 10"
FT REGION 787..825
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 807..825
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 825 AA; 93157 MW; CF0EB73327FD6B99 CRC64;
MVFERTLIDL IKGIRSHAND EEAFIATCLL ECRKEATSQD ADLKSEAILK LAYLEMLGVD
ISWASFQIVE VMSSSKILQK QKGYLAAVQS FKPDTDVLML TTNLLKKDLM SSKVPEITLA
IDGLSHFSTL GLARDLYRDV LILLNHSVPY VRKRTILLLY RLCLQYPEAI SACIPKLRER
LDDPDTSVVN AAVSVICELA RRAPKNYLEF APDLFHLLTT SSNNWMLIKL IKLFASLTPY
EPRLVKKLIP SLTDIIENTH AMSLLYECIN TIVSGNMLVG HSQCDKLASL CASKLRGFFE
DTDQNLKYIA LLCLRKLANT HPSLVSAQLD IILKCLVDTD TSIRLRALDL VNEIVNKENI
RTIVKTLMLQ LIVSSDESAV EDIRNSTATR IIEMTSKSTY MNIADFEWLL TVYVDLANIP
GIDTGTLLNN QIIDLCVRVK ALRPFSVDIF SQAILDPSYV STTDCSVSEK RTDILPAIIW
CLGEYAEFIE EYLDILDALT RPSFKKCSNL AHRLLLQAIT KIFCQWCLEE EPTWGVEKFG
LVKLWVEKIV SFIEQFLNFQ DMEIQRRASE FYILFNQVSD IVNTSDTMEV LELQKKPPYI
VQNTMYKLFF GEPLNPVAVK AQRKVMPDEN LDLNCPINGV IEVPKELLEN IIQSDDSLIN
FDTEVPSSGI DTFSKKQFNS LESVPVQRDL SSPFYLSSNQ HTTTTNSEPE NLNVETSMSD
EAFNADKVTK VTKNKRRRKI FTSPSLQAMV VAQDEVPEGI SLADIENKEN PSNSNVYSLI
SLDPPLSTNQ GSMGDIVLET KSPIRVEKKK SKKKKKKKEK TSGKE
