AP3M1_MOUSE
ID AP3M1_MOUSE Reviewed; 418 AA.
AC Q9JKC8; Q5BKQ6;
DT 10-MAY-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 150.
DE RecName: Full=AP-3 complex subunit mu-1;
DE AltName: Full=AP-3 adaptor complex mu3A subunit;
DE AltName: Full=Adaptor-related protein complex 3 subunit mu-1;
DE AltName: Full=Mu-adaptin 3A;
DE AltName: Full=Mu3A-adaptin;
GN Name=Ap3m1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Werner H., Nave K.-A.;
RT "Mouse medium chain clathrin adaptor protein mu3A.";
RL Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Mammary gland, and Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP INTERACTION WITH AGAP1.
RX PubMed=12967569; DOI=10.1016/s1534-5807(03)00234-x;
RA Nie Z., Boehm M., Boja E.S., Vass W.C., Bonifacino J.S., Fales H.M.,
RA Randazzo P.A.;
RT "Specific regulation of the adaptor protein complex AP-3 by the Arf GAP
RT AGAP1.";
RL Dev. Cell 5:513-521(2003).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Kidney, Lung, Pancreas, Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [5]
RP FUNCTION, AND ASSOCIATION WITH THE BLOC-1 COMPLEX.
RX PubMed=21998198; DOI=10.1091/mbc.e11-07-0592;
RA Larimore J., Tornieri K., Ryder P.V., Gokhale A., Zlatic S.A., Craige B.,
RA Lee J.D., Talbot K., Pare J.F., Smith Y., Faundez V.;
RT "The schizophrenia susceptibility factor dysbindin and its associated
RT complex sort cargoes from cell bodies to the synapse.";
RL Mol. Biol. Cell 22:4854-4867(2011).
CC -!- FUNCTION: Part of the AP-3 complex, an adaptor-related complex which is
CC not clathrin-associated. The complex is associated with the Golgi
CC region as well as more peripheral structures. It facilitates the
CC budding of vesicles from the Golgi membrane and may be directly
CC involved in trafficking to lysosomes. In concert with the BLOC-1
CC complex, AP-3 is required to target cargos into vesicles assembled at
CC cell bodies for delivery into neurites and nerve terminals.
CC {ECO:0000269|PubMed:21998198}.
CC -!- SUBUNIT: Adaptor protein complex 3 (AP-3) is a heterotetramer composed
CC of two large adaptins (delta-type subunit AP3D1 and beta-type subunit
CC AP3B1 or AP3B2), a medium adaptin (mu-type subunit AP3M1 or AP3M2) and
CC a small adaptin (sigma-type subunit APS1 or AP3S2). Interacts with
CC AGAP1. AP-3 associates with the BLOC-1 complex.
CC {ECO:0000269|PubMed:12967569}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus. Cytoplasmic vesicle membrane
CC {ECO:0000250}; Peripheral membrane protein {ECO:0000250}; Cytoplasmic
CC side {ECO:0000250}. Note=Component of the coat surrounding the
CC cytoplasmic face of coated vesicles located at the Golgi complex.
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the adaptor complexes medium subunit family.
CC {ECO:0000305}.
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DR EMBL; AF242857; AAF63512.1; -; mRNA.
DR EMBL; BC024595; AAH24595.1; -; mRNA.
DR EMBL; BC090983; AAH90983.1; -; mRNA.
DR CCDS; CCDS26861.1; -.
DR RefSeq; NP_061299.3; NM_018829.4.
DR RefSeq; XP_006519355.1; XM_006519292.1.
DR RefSeq; XP_006519356.1; XM_006519293.1.
DR AlphaFoldDB; Q9JKC8; -.
DR SMR; Q9JKC8; -.
DR BioGRID; 207741; 3.
DR ComplexPortal; CPX-5145; Ubiquitous AP-3 Adaptor complex, sigma3a variant.
DR ComplexPortal; CPX-5146; Ubiquitous AP-3 Adaptor complex, sigma3b variant.
DR CORUM; Q9JKC8; -.
DR IntAct; Q9JKC8; 4.
DR MINT; Q9JKC8; -.
DR STRING; 10090.ENSMUSP00000117346; -.
DR iPTMnet; Q9JKC8; -.
DR PhosphoSitePlus; Q9JKC8; -.
DR SwissPalm; Q9JKC8; -.
DR EPD; Q9JKC8; -.
DR jPOST; Q9JKC8; -.
DR MaxQB; Q9JKC8; -.
DR PaxDb; Q9JKC8; -.
DR PeptideAtlas; Q9JKC8; -.
DR PRIDE; Q9JKC8; -.
DR ProteomicsDB; 282133; -.
DR Antibodypedia; 45437; 133 antibodies from 28 providers.
DR DNASU; 55946; -.
DR Ensembl; ENSMUST00000154460; ENSMUSP00000117346; ENSMUSG00000021824.
DR GeneID; 55946; -.
DR KEGG; mmu:55946; -.
DR UCSC; uc007sla.2; mouse.
DR CTD; 26985; -.
DR MGI; MGI:1929212; Ap3m1.
DR VEuPathDB; HostDB:ENSMUSG00000021824; -.
DR eggNOG; KOG2740; Eukaryota.
DR GeneTree; ENSGT00940000158184; -.
DR HOGENOM; CLU_026996_6_2_1; -.
DR InParanoid; Q9JKC8; -.
DR OMA; WDIGRID; -.
DR OrthoDB; 928391at2759; -.
DR PhylomeDB; Q9JKC8; -.
DR TreeFam; TF315187; -.
DR BioGRID-ORCS; 55946; 3 hits in 73 CRISPR screens.
DR ChiTaRS; Ap3m1; mouse.
DR PRO; PR:Q9JKC8; -.
DR Proteomes; UP000000589; Chromosome 14.
DR RNAct; Q9JKC8; protein.
DR Bgee; ENSMUSG00000021824; Expressed in ectoplacental cone and 257 other tissues.
DR ExpressionAtlas; Q9JKC8; baseline and differential.
DR Genevisible; Q9JKC8; MM.
DR GO; GO:0030123; C:AP-3 adaptor complex; IC:ComplexPortal.
DR GO; GO:1904115; C:axon cytoplasm; IEA:GOC.
DR GO; GO:0030131; C:clathrin adaptor complex; IEA:InterPro.
DR GO; GO:0031410; C:cytoplasmic vesicle; IBA:GO_Central.
DR GO; GO:0030659; C:cytoplasmic vesicle membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005769; C:early endosome; IC:ComplexPortal.
DR GO; GO:0005794; C:Golgi apparatus; IEA:UniProtKB-SubCell.
DR GO; GO:0031267; F:small GTPase binding; ISO:MGI.
DR GO; GO:0008089; P:anterograde axonal transport; IMP:UniProtKB.
DR GO; GO:0048490; P:anterograde synaptic vesicle transport; IMP:UniProtKB.
DR GO; GO:0035654; P:clathrin-coated vesicle cargo loading, AP-3-mediated; IC:ComplexPortal.
DR GO; GO:0006897; P:endocytosis; IBA:GO_Central.
DR GO; GO:0006886; P:intracellular protein transport; IEA:InterPro.
DR GO; GO:0046907; P:intracellular transport; IC:ComplexPortal.
DR GO; GO:1903232; P:melanosome assembly; IC:ComplexPortal.
DR GO; GO:0060155; P:platelet dense granule organization; IC:ComplexPortal.
DR GO; GO:0016192; P:vesicle-mediated transport; IBA:GO_Central.
DR InterPro; IPR036168; AP2_Mu_C_sf.
DR InterPro; IPR022775; AP_mu_sigma_su.
DR InterPro; IPR001392; Clathrin_mu.
DR InterPro; IPR018240; Clathrin_mu_CS.
DR InterPro; IPR011012; Longin-like_dom_sf.
DR InterPro; IPR028565; MHD.
DR Pfam; PF00928; Adap_comp_sub; 1.
DR Pfam; PF01217; Clat_adaptor_s; 1.
DR PIRSF; PIRSF005992; Clathrin_mu; 1.
DR PRINTS; PR00314; CLATHRINADPT.
DR SUPFAM; SSF49447; SSF49447; 1.
DR SUPFAM; SSF64356; SSF64356; 1.
DR PROSITE; PS00990; CLAT_ADAPTOR_M_1; 1.
DR PROSITE; PS00991; CLAT_ADAPTOR_M_2; 1.
DR PROSITE; PS51072; MHD; 1.
PE 1: Evidence at protein level;
KW Cytoplasmic vesicle; Golgi apparatus; Membrane; Protein transport;
KW Reference proteome; Transport.
FT CHAIN 1..418
FT /note="AP-3 complex subunit mu-1"
FT /id="PRO_0000193782"
FT DOMAIN 176..417
FT /note="MHD"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00404"
SQ SEQUENCE 418 AA; 46936 MW; 69EC322D820C47D3 CRC64;
MIHSLFLINC SGDIFLEKHW KSVVSQSVCD YFFEAQEKAA DVENVPPVIS TPHHYLISIY
RDKLFFVSVI QTEVPPLFVI EFLHRVADTF QDYFGECSEA AIKDNVVIVY ELLEEMLDNG
FPLATESNIL KELIKPPTIL RSVVNSITGS SNVGDTLPTG QLSNIPWRRA GVKYTNNEAY
FDVVEEIDAI IDKSGSTVFA EIQGVIDACI KLSGMPDLSL SFMNPRLLDD VSFHPCIRFK
RWESERVLSF IPPDGNFRLI SYRVSSQNLV AIPVYVKHSI SFKENSSCGR FDITIGPKQN
MGKTIEGITV TVHMPKVVLN MNLTPTQGSY TFDPVTKVLA WDVGKITPQK LPSLKGLVNL
QSGAPKPEEN PNLNIQFKIQ QLAISGLKVN RLDMYGEKYK PFKGVKYVTK AGKFQVRT
