AP3M1_PONAB
ID AP3M1_PONAB Reviewed; 418 AA.
AC Q5R478; Q5RBA0;
DT 01-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 21-DEC-2004, sequence version 1.
DT 25-MAY-2022, entry version 94.
DE RecName: Full=AP-3 complex subunit mu-1;
DE AltName: Full=AP-3 adaptor complex mu3A subunit;
DE AltName: Full=Adaptor-related protein complex 3 subunit mu-1;
DE AltName: Full=Mu-adaptin 3A;
DE AltName: Full=Mu3A-adaptin;
GN Name=AP3M1;
OS Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Pongo.
OX NCBI_TaxID=9601;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC TISSUE=Brain cortex;
RG The German cDNA consortium;
RL Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Part of the AP-3 complex, an adaptor-related complex which is
CC not clathrin-associated. The complex is associated with the Golgi
CC region as well as more peripheral structures. It facilitates the
CC budding of vesicles from the Golgi membrane and may be directly
CC involved in trafficking to lysosomes. In concert with the BLOC-1
CC complex, AP-3 is required to target cargos into vesicles assembled at
CC cell bodies for delivery into neurites and nerve terminals (By
CC similarity). {ECO:0000250}.
CC -!- SUBUNIT: Adaptor protein complex 3 (AP-3) is a heterotetramer composed
CC of two large adaptins (delta-type subunit AP3D1 and beta-type subunit
CC AP3B1 or AP3B2), a medium adaptin (mu-type subunit AP3M1 or AP3M2) and
CC a small adaptin (sigma-type subunit APS1 or AP3S2). Interacts with
CC AGAP1. AP-3 associates with the BLOC-1 complex (By similarity).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus {ECO:0000250}. Cytoplasmic
CC vesicle membrane {ECO:0000250}; Peripheral membrane protein
CC {ECO:0000250}; Cytoplasmic side {ECO:0000250}. Note=Component of the
CC coat surrounding the cytoplasmic face of coated vesicles located at the
CC Golgi complex. {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q5R478-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q5R478-2; Sequence=VSP_024916, VSP_024917;
CC -!- SIMILARITY: Belongs to the adaptor complexes medium subunit family.
CC {ECO:0000305}.
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DR EMBL; CR858751; CAH90960.1; -; mRNA.
DR EMBL; CR861379; CAH93438.1; -; mRNA.
DR RefSeq; NP_001125552.1; NM_001132080.1.
DR RefSeq; NP_001127013.1; NM_001133541.1. [Q5R478-1]
DR RefSeq; XP_009243726.1; XM_009245451.1.
DR RefSeq; XP_009243727.1; XM_009245452.1.
DR AlphaFoldDB; Q5R478; -.
DR SMR; Q5R478; -.
DR STRING; 9601.ENSPPYP00000002697; -.
DR Ensembl; ENSPPYT00000043470; ENSPPYP00000045734; ENSPPYG00000002322. [Q5R478-2]
DR GeneID; 100172465; -.
DR GeneID; 100174037; -.
DR KEGG; pon:100174037; -.
DR CTD; 26985; -.
DR eggNOG; KOG2740; Eukaryota.
DR GeneTree; ENSGT00940000158184; -.
DR HOGENOM; CLU_026996_6_2_1; -.
DR InParanoid; Q5R478; -.
DR OMA; WDIGRID; -.
DR OrthoDB; 928391at2759; -.
DR TreeFam; TF315187; -.
DR Proteomes; UP000001595; Chromosome 10.
DR GO; GO:1904115; C:axon cytoplasm; IEA:GOC.
DR GO; GO:0030131; C:clathrin adaptor complex; IEA:InterPro.
DR GO; GO:0030659; C:cytoplasmic vesicle membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005794; C:Golgi apparatus; IEA:UniProtKB-SubCell.
DR GO; GO:0031267; F:small GTPase binding; IEA:Ensembl.
DR GO; GO:0008089; P:anterograde axonal transport; ISS:UniProtKB.
DR GO; GO:0048490; P:anterograde synaptic vesicle transport; ISS:UniProtKB.
DR GO; GO:0006886; P:intracellular protein transport; IEA:InterPro.
DR GO; GO:0016192; P:vesicle-mediated transport; IEA:InterPro.
DR InterPro; IPR036168; AP2_Mu_C_sf.
DR InterPro; IPR022775; AP_mu_sigma_su.
DR InterPro; IPR001392; Clathrin_mu.
DR InterPro; IPR018240; Clathrin_mu_CS.
DR InterPro; IPR011012; Longin-like_dom_sf.
DR InterPro; IPR028565; MHD.
DR Pfam; PF00928; Adap_comp_sub; 1.
DR Pfam; PF01217; Clat_adaptor_s; 1.
DR PIRSF; PIRSF005992; Clathrin_mu; 1.
DR PRINTS; PR00314; CLATHRINADPT.
DR SUPFAM; SSF49447; SSF49447; 1.
DR SUPFAM; SSF64356; SSF64356; 1.
DR PROSITE; PS00990; CLAT_ADAPTOR_M_1; 1.
DR PROSITE; PS00991; CLAT_ADAPTOR_M_2; 1.
DR PROSITE; PS51072; MHD; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cytoplasmic vesicle; Golgi apparatus; Membrane;
KW Protein transport; Reference proteome; Transport.
FT CHAIN 1..418
FT /note="AP-3 complex subunit mu-1"
FT /id="PRO_0000285805"
FT DOMAIN 176..417
FT /note="MHD"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00404"
FT VAR_SEQ 339..361
FT /note="LTWDVGKITPQKLPSLKGLVNLQ -> QPLQIKSTNMGCGKNYSTKAPKS
FT (in isoform 2)"
FT /evidence="ECO:0000303|Ref.1"
FT /id="VSP_024916"
FT VAR_SEQ 362..418
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|Ref.1"
FT /id="VSP_024917"
FT CONFLICT 108
FT /note="I -> T (in Ref. 1; CAH90960)"
FT /evidence="ECO:0000305"
FT CONFLICT 261
FT /note="S -> P (in Ref. 1; CAH90960)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 418 AA; 46939 MW; 533E189C320C4C48 CRC64;
MIHSLFLINC SGDIFLEKHW KSVVSQSVCD YFFEAQEKAA DVENVPPVIS TPHHYLISIY
RDKLFFVSVI QTEVPPLFVI EFLHRVADTF QDYFGECSEA AIKDNVVIVY ELLEEMLDNG
FPLATESNIL KELIKPPTIL RSVVNSITGS SNVGDTLPTG QLSNIPWRRA GVKYTNNEAY
FDVVEEIDAI IDKSGSTVFA EIQGVIDACI KLSGMPDLSL SFMNPRLLDD VSFHPCIRFK
RWESERVLSF IPPDGNFRLI SYRVSSQNLV AIPVYVKHSI SFKENSSCGR FDITIGPKQN
MGKTIEGITV TVHMPKVVLN MNLTPTQGSY TFDPVTKVLT WDVGKITPQK LPSLKGLVNL
QSGAPKPEEN PSLNIQFKIQ QLAISGLKVN RLDMYGEKYK PFKGVKYVTK AGKFQVRT
