AP3M2_RAT
ID AP3M2_RAT Reviewed; 418 AA.
AC P53678;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 03-AUG-2022, entry version 134.
DE RecName: Full=AP-3 complex subunit mu-2;
DE AltName: Full=Adaptor-related protein complex 3 subunit mu-2;
DE AltName: Full=Clathrin assembly protein assembly protein complex 3 mu-2 medium chain;
DE AltName: Full=Clathrin coat assembly protein AP47 homolog 2;
DE AltName: Full=Clathrin coat-associated protein AP47 homolog 2;
DE AltName: Full=Golgi adaptor AP-1 47 kDa protein homolog 2;
DE AltName: Full=HA1 47 kDa subunit homolog 2;
DE AltName: Full=Mu3B-adaptin;
DE AltName: Full=P47B;
GN Name=Ap3m2;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Brain cortex;
RX PubMed=8076832; DOI=10.1016/0378-1119(94)90306-9;
RA Pevsner J., Volknandt W., Wong B.R., Scheller R.H.;
RT "Two rat homologs of clathrin-associated adaptor proteins.";
RL Gene 146:279-283(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Component of the adaptor complexes which link clathrin to
CC receptors in coated vesicles. Clathrin-associated protein complexes are
CC believed to interact with the cytoplasmic tails of membrane proteins,
CC leading to their selection and concentration. Ap47 is a subunit of the
CC plasma membrane adaptor. In concert with the BLOC-1 complex, AP-3 is
CC required to target cargos into vesicles assembled at cell bodies for
CC delivery into neurites and nerve terminals (By similarity).
CC {ECO:0000250}.
CC -!- SUBUNIT: Adaptor protein complex 3 (AP-3) is a heterotetramer composed
CC of two large adaptins (delta-type subunit AP3D1 and beta-type subunit
CC AP3B1 or AP3B2), a medium adaptin (mu-type subunit AP3M1 or AP3M2) and
CC a small adaptin (sigma-type subunit APS1 or AP3S2). AP-3 associates
CC with the BLOC-1 complex (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus. Cytoplasmic vesicle membrane;
CC Peripheral membrane protein; Cytoplasmic side. Note=Component of the
CC coat surrounding the cytoplasmic face of coated vesicles located at the
CC Golgi complex.
CC -!- SIMILARITY: Belongs to the adaptor complexes medium subunit family.
CC {ECO:0000305}.
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DR EMBL; L07074; AAA57232.1; -; mRNA.
DR EMBL; BC086993; AAH86993.1; -; mRNA.
DR PIR; I63224; I63224.
DR RefSeq; NP_579839.1; NM_133305.2.
DR AlphaFoldDB; P53678; -.
DR SMR; P53678; -.
DR BioGRID; 250827; 1.
DR STRING; 10116.ENSRNOP00000025675; -.
DR PhosphoSitePlus; P53678; -.
DR jPOST; P53678; -.
DR PRIDE; P53678; -.
DR Ensembl; ENSRNOT00000025675; ENSRNOP00000025675; ENSRNOG00000018650.
DR GeneID; 140667; -.
DR KEGG; rno:140667; -.
DR UCSC; RGD:70969; rat.
DR CTD; 10947; -.
DR RGD; 70969; Ap3m2.
DR InParanoid; P53678; -.
DR OrthoDB; 928391at2759; -.
DR PhylomeDB; P53678; -.
DR TreeFam; TF315187; -.
DR PRO; PR:P53678; -.
DR Proteomes; UP000002494; Chromosome 16.
DR GO; GO:1904115; C:axon cytoplasm; IEA:GOC.
DR GO; GO:0030131; C:clathrin adaptor complex; IEA:InterPro.
DR GO; GO:0031410; C:cytoplasmic vesicle; IBA:GO_Central.
DR GO; GO:0030659; C:cytoplasmic vesicle membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005794; C:Golgi apparatus; IEA:UniProtKB-SubCell.
DR GO; GO:0098793; C:presynapse; IEA:GOC.
DR GO; GO:0008089; P:anterograde axonal transport; ISS:UniProtKB.
DR GO; GO:0048490; P:anterograde synaptic vesicle transport; ISS:UniProtKB.
DR GO; GO:0006897; P:endocytosis; IBA:GO_Central.
DR GO; GO:0006886; P:intracellular protein transport; IEA:InterPro.
DR GO; GO:0046907; P:intracellular transport; NAS:RGD.
DR GO; GO:0007269; P:neurotransmitter secretion; NAS:RGD.
DR GO; GO:0016192; P:vesicle-mediated transport; IBA:GO_Central.
DR InterPro; IPR036168; AP2_Mu_C_sf.
DR InterPro; IPR022775; AP_mu_sigma_su.
DR InterPro; IPR001392; Clathrin_mu.
DR InterPro; IPR018240; Clathrin_mu_CS.
DR InterPro; IPR011012; Longin-like_dom_sf.
DR InterPro; IPR028565; MHD.
DR Pfam; PF00928; Adap_comp_sub; 1.
DR Pfam; PF01217; Clat_adaptor_s; 1.
DR PIRSF; PIRSF005992; Clathrin_mu; 1.
DR PRINTS; PR00314; CLATHRINADPT.
DR SUPFAM; SSF49447; SSF49447; 1.
DR SUPFAM; SSF64356; SSF64356; 1.
DR PROSITE; PS00990; CLAT_ADAPTOR_M_1; 1.
DR PROSITE; PS00991; CLAT_ADAPTOR_M_2; 1.
DR PROSITE; PS51072; MHD; 1.
PE 2: Evidence at transcript level;
KW Cytoplasmic vesicle; Golgi apparatus; Membrane; Protein transport;
KW Reference proteome; Transport.
FT CHAIN 1..418
FT /note="AP-3 complex subunit mu-2"
FT /id="PRO_0000193786"
FT DOMAIN 176..417
FT /note="MHD"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00404"
SQ SEQUENCE 418 AA; 46874 MW; 50AA5A2D63549D07 CRC64;
MIHSLFLINS AGDIFLEKHW KSVVSRSVCD YFFEAQERAT EAENVPPVIP TPHHYLLSVY
RHKIFFVAVI QTEVPPLFVI EFLHRVVDTF QDYFGVCSEP VIKDNVVVVY EVLEEMLDNG
FPLATESNIL KELIKPPTIL RTVVNTITGS TNVGDQLPTG QLSVVPWRRT GVKYTNNEAY
FDVVEEIDAI IDKSGSTVTA EIQGVIDACV KLTGMPDLTL SFMNPRLLDD VSFHPCVRFK
RWESERILSF IPPDGNFRLL SYHVSAQNLV AIPVYVKHSI SFRDSGSLGR FEITVGPKQT
MGKTIEGVTV TSQMPKGVLN MSLTPSQGTH TFDPVTKMLS WDVGKINPQK LPSLKGTMGL
QVGASKPDEN PTINLQFKIQ QLAISGLKVN RLDMYGEKYK PFKGIKYMTK AGKFQVRT
