ID   AP3M_ASHGO              Reviewed;         451 AA.
AC   Q750L8;
DT   21-MAR-2006, integrated into UniProtKB/Swiss-Prot.
DT   09-JAN-2013, sequence version 2.
DT   03-AUG-2022, entry version 108.
DE   RecName: Full=AP-3 complex subunit mu;
DE   AltName: Full=AP-3 adaptor complex mu3A subunit;
DE   AltName: Full=Adaptor-related protein complex 3 subunit mu;
DE   AltName: Full=Mu-adaptin 3A;
DE   AltName: Full=Mu3-adaptin;
GN   Name=APM3; OrderedLocusNames=AGL061W;
OS   Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)
OS   (Yeast) (Eremothecium gossypii).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Eremothecium.
OX   NCBI_TaxID=284811;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=15001715; DOI=10.1126/science.1095781;
RA   Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S.,
RA   Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A.,
RA   Gaffney T.D., Philippsen P.;
RT   "The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces
RT   cerevisiae genome.";
RL   Science 304:304-307(2004).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056;
RX   PubMed=23749448; DOI=10.1534/g3.112.002881;
RA   Dietrich F.S., Voegeli S., Kuo S., Philippsen P.;
RT   "Genomes of Ashbya fungi isolated from insects reveal four mating-type
RT   loci, numerous translocations, lack of transposons, and distinct gene
RT   duplications.";
RL   G3 (Bethesda) 3:1225-1239(2013).
CC   -!- FUNCTION: Part of the AP-3 complex, an adaptor-related complex which is
CC       not clathrin-associated. The complex is associated with the Golgi
CC       region as well as more peripheral structures. It facilitates the
CC       budding of vesicles from the Golgi membrane and may be directly
CC       involved in trafficking to the vacuole (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Adaptor protein complex 3 (AP-3) is a heterotetramer composed
CC       of 2 large adaptins (APL5 and APL6), a medium adaptin (APM3) and a
CC       small adaptin (APS3). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Golgi apparatus {ECO:0000250|UniProtKB:P38153}.
CC       Cytoplasmic vesicle membrane {ECO:0000250|UniProtKB:P38153}; Peripheral
CC       membrane protein {ECO:0000250|UniProtKB:P38153}; Cytoplasmic side
CC       {ECO:0000250|UniProtKB:P38153}. Note=Component of the coat surrounding
CC       the cytoplasmic face of coated vesicles located at the Golgi complex.
CC       {ECO:0000250|UniProtKB:P38153}.
CC   -!- SIMILARITY: Belongs to the adaptor complexes medium subunit family.
CC       {ECO:0000305}.
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DR   EMBL; AE016820; AAS54429.2; -; Genomic_DNA.
DR   RefSeq; NP_986605.2; NM_211667.2.
DR   AlphaFoldDB; Q750L8; -.
DR   SMR; Q750L8; -.
DR   STRING; 33169.AAS54429; -.
DR   PRIDE; Q750L8; -.
DR   EnsemblFungi; AAS54429; AAS54429; AGOS_AGL061W.
DR   GeneID; 4622904; -.
DR   KEGG; ago:AGOS_AGL061W; -.
DR   eggNOG; KOG2740; Eukaryota.
DR   HOGENOM; CLU_026449_0_0_1; -.
DR   InParanoid; Q750L8; -.
DR   OMA; TLNTNFP; -.
DR   Proteomes; UP000000591; Chromosome VII.
DR   GO; GO:0030123; C:AP-3 adaptor complex; IEA:EnsemblFungi.
DR   GO; GO:0030131; C:clathrin adaptor complex; IEA:InterPro.
DR   GO; GO:0031410; C:cytoplasmic vesicle; IBA:GO_Central.
DR   GO; GO:0030659; C:cytoplasmic vesicle membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005794; C:Golgi apparatus; IEA:UniProtKB-SubCell.
DR   GO; GO:0006897; P:endocytosis; IBA:GO_Central.
DR   GO; GO:0006896; P:Golgi to vacuole transport; IEA:EnsemblFungi.
DR   GO; GO:0006623; P:protein targeting to vacuole; IEA:EnsemblFungi.
DR   GO; GO:0016192; P:vesicle-mediated transport; IBA:GO_Central.
DR   InterPro; IPR036168; AP2_Mu_C_sf.
DR   InterPro; IPR001392; Clathrin_mu.
DR   InterPro; IPR018240; Clathrin_mu_CS.
DR   InterPro; IPR011012; Longin-like_dom_sf.
DR   InterPro; IPR028565; MHD.
DR   Pfam; PF00928; Adap_comp_sub; 1.
DR   PIRSF; PIRSF005992; Clathrin_mu; 1.
DR   SUPFAM; SSF49447; SSF49447; 1.
DR   SUPFAM; SSF64356; SSF64356; 1.
DR   PROSITE; PS00990; CLAT_ADAPTOR_M_1; 1.
DR   PROSITE; PS51072; MHD; 1.
PE   3: Inferred from homology;
KW   Cytoplasmic vesicle; Golgi apparatus; Membrane; Protein transport;
KW   Reference proteome; Transport.
FT   CHAIN           1..451
FT                   /note="AP-3 complex subunit mu"
FT                   /id="PRO_0000227678"
FT   DOMAIN          191..450
FT                   /note="MHD"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00404"
SQ   SEQUENCE   451 AA;  49229 MW;  BCC2505850611713 CRC64;
     MYTSIYITDS KKNLVFEYLL TSQAPTFQQL CSKIAGRDAA MDAMVAISKD MSLYRQAVGP
     EKLWYWALCQ ACGDPLEPQM FLAQYHQVLI EYFDKEALTV KKLVNNADRL ALLLHAMLDA
     GEVAVTDSNR LRQLVPLRND LSTILNSATK TLANTVKYAD SKQLFGAPVA TGKVEAGQTV
     PWRTADCRYV NNEIYVDLVE TVNATLRQKG SSLTLINGSL SGKIDVKCYL SGNPTVQLKL
     RTSGHPLDNS ALHRCVELGE AGVATMNFVP PDGRFTLAEY AIDLSAISQA ARRLTNLGLV
     TVSLASGLGQ HRDEFEIKVI IGNSTQVAAI EDLRITVYFP DISDAAKIKI LRTTHGGWES
     DLSRVRGVWA FDKQTAVGSV PVLRGCVENP ESTPHAPPVF PSHLAVSYSH VGQLPSGIRV
     DTIALSDLPP GSKPFKGVKY TSRAGDYIVR A