AP3M_DICDI
ID AP3M_DICDI Reviewed; 421 AA.
AC Q9GPF1; Q54Y74;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 25-MAY-2022, entry version 108.
DE RecName: Full=AP-3 complex subunit mu;
DE AltName: Full=AP-3 adaptor complex mu3 subunit;
DE AltName: Full=Adaptor-related protein complex 3 subunit mu;
DE AltName: Full=Clathrin-adaptor medium chain Apm3;
DE AltName: Full=Mu3-adaptin;
GN Name=apm3; ORFNames=DDB_G0277901;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND DEVELOPMENTAL STAGE.
RX PubMed=11179674; DOI=10.1016/s0378-1119(00)00545-x;
RA de Chassey B., Dubois A., Lefkir Y., Letourneur F.;
RT "Identification of clathrin-adaptor medium chains in Dictyostelium
RT discoideum: differential expression during development.";
RL Gene 262:115-122(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [3]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=17010123; DOI=10.1111/j.1600-0854.2006.00478.x;
RA Charette S.J., Mercanti V., Letourneur F., Bennett N., Cosson P.;
RT "A role for adaptor protein-3 complex in the organization of the endocytic
RT pathway in Dictyostelium.";
RL Traffic 7:1528-1538(2006).
CC -!- FUNCTION: Part of the AP-3 complex, an adaptor-related complex which is
CC essential for the compartmentalization of the endocytic pathway.
CC {ECO:0000269|PubMed:17010123}.
CC -!- SUBUNIT: Adaptor protein complex 3 (AP-3) is a heterotetramer composed
CC of two large adaptins (delta-type subunit and beta-type subunit), a
CC medium adaptin (mu-type subunit) and a small adaptin (sigma-type
CC subunit). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Endosome membrane {ECO:0000250}.
CC -!- DEVELOPMENTAL STAGE: Constant expression throughout development.
CC {ECO:0000269|PubMed:11179674}.
CC -!- DISRUPTION PHENOTYPE: Cells are viable but are unable to retrieve
CC efficiently some proteins from early endocytic compartments.
CC {ECO:0000269|PubMed:17010123}.
CC -!- SIMILARITY: Belongs to the adaptor complexes medium subunit family.
CC {ECO:0000305}.
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DR EMBL; AY007279; AAG11392.1; -; mRNA.
DR EMBL; AAFI02000023; EAL68123.1; -; Genomic_DNA.
DR RefSeq; XP_642328.1; XM_637236.2.
DR AlphaFoldDB; Q9GPF1; -.
DR SMR; Q9GPF1; -.
DR STRING; 44689.DDB0214930; -.
DR PaxDb; Q9GPF1; -.
DR EnsemblProtists; EAL68123; EAL68123; DDB_G0277901.
DR GeneID; 8621534; -.
DR KEGG; ddi:DDB_G0277901; -.
DR dictyBase; DDB_G0277901; apm3.
DR eggNOG; KOG2740; Eukaryota.
DR HOGENOM; CLU_026996_6_2_1; -.
DR InParanoid; Q9GPF1; -.
DR OMA; WDIGRID; -.
DR PhylomeDB; Q9GPF1; -.
DR PRO; PR:Q9GPF1; -.
DR Proteomes; UP000002195; Chromosome 3.
DR GO; GO:0030123; C:AP-3 adaptor complex; IDA:dictyBase.
DR GO; GO:0030131; C:clathrin adaptor complex; IEA:InterPro.
DR GO; GO:0031410; C:cytoplasmic vesicle; IBA:GO_Central.
DR GO; GO:0005768; C:endosome; ISS:dictyBase.
DR GO; GO:0010008; C:endosome membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005764; C:lysosome; ISS:dictyBase.
DR GO; GO:0031201; C:SNARE complex; IDA:dictyBase.
DR GO; GO:0009267; P:cellular response to starvation; IMP:dictyBase.
DR GO; GO:0006897; P:endocytosis; IBA:GO_Central.
DR GO; GO:0007032; P:endosome organization; IMP:dictyBase.
DR GO; GO:0008333; P:endosome to lysosome transport; IMP:dictyBase.
DR GO; GO:0006886; P:intracellular protein transport; ISS:dictyBase.
DR GO; GO:0007040; P:lysosome organization; IMP:dictyBase.
DR GO; GO:0006907; P:pinocytosis; IMP:dictyBase.
DR GO; GO:0016192; P:vesicle-mediated transport; IBA:GO_Central.
DR InterPro; IPR036168; AP2_Mu_C_sf.
DR InterPro; IPR001392; Clathrin_mu.
DR InterPro; IPR018240; Clathrin_mu_CS.
DR InterPro; IPR011012; Longin-like_dom_sf.
DR InterPro; IPR028565; MHD.
DR Pfam; PF00928; Adap_comp_sub; 1.
DR PIRSF; PIRSF005992; Clathrin_mu; 1.
DR PRINTS; PR00314; CLATHRINADPT.
DR SUPFAM; SSF49447; SSF49447; 1.
DR SUPFAM; SSF64356; SSF64356; 1.
DR PROSITE; PS00991; CLAT_ADAPTOR_M_2; 1.
DR PROSITE; PS51072; MHD; 1.
PE 2: Evidence at transcript level;
KW Endosome; Membrane; Protein transport; Reference proteome; Transport.
FT CHAIN 1..421
FT /note="AP-3 complex subunit mu"
FT /id="PRO_0000327980"
FT DOMAIN 178..420
FT /note="MHD"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00404"
SQ SEQUENCE 421 AA; 47144 MW; 7268F2F2F4859BCD CRC64;
MLSSFFIIAD QGDILIEKHW RGLMNRSICE YFWDQVLQSK QNGSSVPPII STPKYYLINI
QKQNVYLLGV CQSEVSPLLV VDFLQRIYDT FVEYFGSNIT SATIKENFVH VYQLLDEMAD
NGFPFTTELN FLKEMIKPPG VLSNVISSVT GTSNITDILP NGSLGAIQWR KTGIKYTQNK
IFFDIIEEID CIIDSNGYIV SSEINGEILC HCNLSGMPDL TMTFNNPRML DDVSFHPCVR
YSRWENDRVL SFIPPDGNFK LLSYRVKGIN QFPVYVKPQI SYSEGSSSVG RVNVTVGAKG
YNVQNKLSIE DVVATIPFSK TTSSTNLTAN IGSFGMDEQS KILRWNIGKI PKEKTPFLNG
TVSLIAGSMT PESTPSIMLQ FKIPQYAISG LTIDSLACSE RYKPFKGVKC TTKAGKFQVR
S
