AP3S1_HUMAN
ID AP3S1_HUMAN Reviewed; 193 AA.
AC Q92572; O00647; O00676; O00721; O00727; Q53XL4; Q6ICQ2;
DT 19-JUL-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1997, sequence version 1.
DT 03-AUG-2022, entry version 177.
DE RecName: Full=AP-3 complex subunit sigma-1;
DE AltName: Full=AP-3 complex subunit sigma-3A;
DE AltName: Full=Adaptor-related protein complex 3 subunit sigma-1;
DE AltName: Full=Clathrin-associated/assembly/adaptor protein, small 3;
DE AltName: Full=Sigma-3A-adaptin;
DE Short=Sigma3A-adaptin;
DE AltName: Full=Sigma-adaptin 3a;
GN Name=AP3S1; Synonyms=CLAPS3;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RC TISSUE=Fetal brain;
RX PubMed=8697810; DOI=10.1159/000134341;
RA Watanabe T.K., Shimizu F., Nagata M., Takaichi A., Fujiwara T.,
RA Nakamura Y., Takahashi E., Hirai Y.;
RT "Cloning, expression pattern and mapping to 12p 13.2 --> p13.1 of CLAPS3, a
RT gene encoding a novel clathrin-adaptor small chain.";
RL Cytogenet. Cell Genet. 73:214-217(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RC TISSUE=Placenta;
RX PubMed=9151686; DOI=10.1083/jcb.137.4.835;
RA Simpson F., Peden A.A., Christopoulou L., Robinson M.S.;
RT "Characterization of the adaptor-related protein complex, AP-3.";
RL J. Cell Biol. 137:835-845(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RC TISSUE=Brain, Pancreas, and Placenta;
RX PubMed=9118953; DOI=10.1093/emboj/16.5.917;
RA Dell'Angelica E.C., Ohno H., Ooi C.E., Rabinovich E., Roche K.W.,
RA Bonifacino J.S.;
RT "AP-3: an adaptor-like protein complex with ubiquitous expression.";
RL EMBO J. 16:917-928(1997).
RN [4] {ECO:0000312|EMBL:AAH00804.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S.,
RA Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y.,
RA Phelan M., Farmer A.;
RT "Cloning of human full-length CDSs in BD Creator(TM) system donor vector.";
RL Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases.
RN [5] {ECO:0000312|EMBL:AAH00804.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.;
RT "Cloning of human full open reading frames in Gateway(TM) system entry
RT vector (pDONR201).";
RL Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Placenta;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T.,
RA Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [8]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-191, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Erythroleukemia;
RX PubMed=23186163; DOI=10.1021/pr300630k;
RA Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA Mohammed S.;
RT "Toward a comprehensive characterization of a human cancer cell
RT phosphoproteome.";
RL J. Proteome Res. 12:260-271(2013).
CC -!- FUNCTION: Part of the AP-3 complex, an adaptor-related complex which is
CC not clathrin-associated. The complex is associated with the Golgi
CC region as well as more peripheral structures. It facilitates the
CC budding of vesicles from the Golgi membrane and may be directly
CC involved in trafficking to lysosomes. In concert with the BLOC-1
CC complex, AP-3 is required to target cargos into vesicles assembled at
CC cell bodies for delivery into neurites and nerve terminals.
CC -!- SUBUNIT: Adaptor protein complex 3 (AP-3) is a heterotetramer composed
CC of two large adaptins (delta-type subunit AP3D1 and beta-type subunit
CC AP3B1 or AP3B2), a medium adaptin (mu-type subunit AP3M1 or AP3M2) and
CC a small adaptin (sigma-type subunit APS1 or AP3S2). Interacts with
CC AGAP1. AP-3 associates with the BLOC-1 complex (By similarity).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus. Cytoplasmic vesicle membrane;
CC Peripheral membrane protein; Cytoplasmic side. Note=Component of the
CC coat surrounding the cytoplasmic face of coated vesicles located at the
CC Golgi complex.
CC -!- TISSUE SPECIFICITY: Present in all adult tissues examined.
CC {ECO:0000269|PubMed:8697810, ECO:0000269|PubMed:9118953,
CC ECO:0000269|PubMed:9151686}.
CC -!- SIMILARITY: Belongs to the adaptor complexes small subunit family.
CC {ECO:0000305}.
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DR EMBL; D63643; BAA09798.1; -; mRNA.
DR EMBL; U91932; AAD03779.1; -; mRNA.
DR EMBL; X99458; CAA67823.1; -; mRNA.
DR EMBL; BT009833; AAP88835.1; -; mRNA.
DR EMBL; CR450341; CAG29337.1; -; mRNA.
DR EMBL; BC000804; AAH00804.1; -; mRNA.
DR CCDS; CCDS4123.1; -.
DR RefSeq; NP_001002924.2; NM_001002924.2.
DR RefSeq; NP_001275.1; NM_001284.3.
DR RefSeq; NP_001305019.1; NM_001318090.1.
DR RefSeq; NP_001305020.1; NM_001318091.1.
DR RefSeq; NP_001305022.1; NM_001318093.1.
DR RefSeq; NP_001305023.1; NM_001318094.1.
DR AlphaFoldDB; Q92572; -.
DR SMR; Q92572; -.
DR BioGRID; 107590; 100.
DR ComplexPortal; CPX-5051; Ubiquitous AP-3 Adaptor complex, sigma3a variant.
DR ComplexPortal; CPX-5055; Neuronal AP-3 Adaptor complex, sigma3a variant.
DR CORUM; Q92572; -.
DR IntAct; Q92572; 50.
DR STRING; 9606.ENSP00000325369; -.
DR GlyGen; Q92572; 1 site, 1 O-linked glycan (1 site).
DR iPTMnet; Q92572; -.
DR MetOSite; Q92572; -.
DR PhosphoSitePlus; Q92572; -.
DR BioMuta; AP3S1; -.
DR EPD; Q92572; -.
DR jPOST; Q92572; -.
DR MassIVE; Q92572; -.
DR MaxQB; Q92572; -.
DR PaxDb; Q92572; -.
DR PeptideAtlas; Q92572; -.
DR PRIDE; Q92572; -.
DR ProteomicsDB; 75333; -.
DR TopDownProteomics; Q92572; -.
DR Antibodypedia; 25448; 130 antibodies from 22 providers.
DR DNASU; 1176; -.
DR Ensembl; ENST00000316788.12; ENSP00000325369.7; ENSG00000177879.17.
DR GeneID; 1176; -.
DR KEGG; hsa:1176; -.
DR MANE-Select; ENST00000316788.12; ENSP00000325369.7; NM_001284.4; NP_001275.1.
DR UCSC; uc003krl.4; human.
DR CTD; 1176; -.
DR DisGeNET; 1176; -.
DR GeneCards; AP3S1; -.
DR HGNC; HGNC:2013; AP3S1.
DR HPA; ENSG00000177879; Low tissue specificity.
DR MIM; 601507; gene.
DR neXtProt; NX_Q92572; -.
DR OpenTargets; ENSG00000177879; -.
DR PharmGKB; PA24862; -.
DR VEuPathDB; HostDB:ENSG00000177879; -.
DR eggNOG; KOG0936; Eukaryota.
DR GeneTree; ENSGT00970000193421; -.
DR HOGENOM; CLU_061221_2_2_1; -.
DR InParanoid; Q92572; -.
DR OMA; ERCFKNV; -.
DR OrthoDB; 1458249at2759; -.
DR PhylomeDB; Q92572; -.
DR TreeFam; TF300189; -.
DR PathwayCommons; Q92572; -.
DR Reactome; R-HSA-432722; Golgi Associated Vesicle Biogenesis.
DR SignaLink; Q92572; -.
DR SIGNOR; Q92572; -.
DR BioGRID-ORCS; 1176; 71 hits in 1068 CRISPR screens.
DR ChiTaRS; AP3S1; human.
DR GeneWiki; AP3S1; -.
DR GenomeRNAi; 1176; -.
DR Pharos; Q92572; Tbio.
DR PRO; PR:Q92572; -.
DR Proteomes; UP000005640; Chromosome 5.
DR RNAct; Q92572; protein.
DR Bgee; ENSG00000177879; Expressed in calcaneal tendon and 95 other tissues.
DR ExpressionAtlas; Q92572; baseline and differential.
DR Genevisible; Q92572; HS.
DR GO; GO:0030123; C:AP-3 adaptor complex; IDA:FlyBase.
DR GO; GO:0030119; C:AP-type membrane coat adaptor complex; TAS:ProtInc.
DR GO; GO:1904115; C:axon cytoplasm; IEA:GOC.
DR GO; GO:0030659; C:cytoplasmic vesicle membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005769; C:early endosome; IC:ComplexPortal.
DR GO; GO:0005794; C:Golgi apparatus; IEA:UniProtKB-SubCell.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; IDA:HPA.
DR GO; GO:0098793; C:presynapse; IEA:GOC.
DR GO; GO:0030133; C:transport vesicle; TAS:ProtInc.
DR GO; GO:0008089; P:anterograde axonal transport; ISS:UniProtKB.
DR GO; GO:0048490; P:anterograde synaptic vesicle transport; ISS:UniProtKB.
DR GO; GO:0035654; P:clathrin-coated vesicle cargo loading, AP-3-mediated; IC:ComplexPortal.
DR GO; GO:0006896; P:Golgi to vacuole transport; IEA:InterPro.
DR GO; GO:0008286; P:insulin receptor signaling pathway; TAS:ProtInc.
DR GO; GO:0006886; P:intracellular protein transport; IEA:InterPro.
DR GO; GO:0046907; P:intracellular transport; IC:ComplexPortal.
DR GO; GO:1903232; P:melanosome assembly; IC:ComplexPortal.
DR GO; GO:0060155; P:platelet dense granule organization; IC:ComplexPortal.
DR GO; GO:0016183; P:synaptic vesicle coating; IC:ComplexPortal.
DR GO; GO:0036465; P:synaptic vesicle recycling; IC:ComplexPortal.
DR GO; GO:0016192; P:vesicle-mediated transport; IBA:GO_Central.
DR CDD; cd14834; AP3_sigma; 1.
DR InterPro; IPR016635; AP_complex_ssu.
DR InterPro; IPR022775; AP_mu_sigma_su.
DR InterPro; IPR027155; APS3.
DR InterPro; IPR000804; Clathrin_sm-chain_CS.
DR InterPro; IPR011012; Longin-like_dom_sf.
DR PANTHER; PTHR11753; PTHR11753; 1.
DR Pfam; PF01217; Clat_adaptor_s; 1.
DR SUPFAM; SSF64356; SSF64356; 1.
DR PROSITE; PS00989; CLAT_ADAPTOR_S; 1.
PE 1: Evidence at protein level;
KW Cytoplasmic vesicle; Golgi apparatus; Membrane; Phosphoprotein;
KW Protein transport; Reference proteome; Transport.
FT CHAIN 1..193
FT /note="AP-3 complex subunit sigma-1"
FT /id="PRO_0000193815"
FT MOD_RES 191
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:23186163"
FT VARIANT 158
FT /note="P -> L (in dbSNP:rs7733604)"
FT /id="VAR_059111"
SQ SEQUENCE 193 AA; 21732 MW; 1EF17F375881288D CRC64;
MIKAILIFNN HGKPRLSKFY QPYSEDTQQQ IIRETFHLVS KRDENVCNFL EGGLLIGGSD
NKLIYRHYAT LYFVFCVDSS ESELGILDLI QVFVETLDKC FENVCELDLI FHVDKVHNIL
AEMVMGGMVL ETNMNEIVTQ IDAQNKLEKS EAGLAGAPAR AVSAVKNMNL PEIPRNINIG
DISIKVPNLP SFK
