ID   AP3S_ARATH              Reviewed;         166 AA.
AC   Q8VZ37; Q9SVL3;
DT   05-OCT-2010, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2002, sequence version 1.
DT   25-MAY-2022, entry version 112.
DE   RecName: Full=AP-3 complex subunit sigma;
DE   AltName: Full=AP-3 complex subunit sigma-3;
DE   AltName: Full=Adaptor-related protein complex 3 subunit sigma;
DE   AltName: Full=Sigma-adaptin 3;
DE   AltName: Full=Sigma3-adaptin;
GN   OrderedLocusNames=At3g50860; ORFNames=F18B3.140;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130713; DOI=10.1038/35048706;
RA   Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA   Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA   Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA   Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA   Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA   Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA   Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA   Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA   Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA   Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA   Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA   Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA   de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA   Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA   Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA   Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA   Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA   Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA   Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA   Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA   Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA   Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA   Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA   Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT   "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL   Nature 408:820-822(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA   Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA   Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA   Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA   Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA   Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA   Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA   Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA   Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA   Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA   Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA   Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [4]
RP   GENE FAMILY, AND REVIEW.
RX   PubMed=11598180; DOI=10.1091/mbc.12.10.2907;
RA   Boehm M., Bonifacino J.S.;
RT   "Adaptins: the final recount.";
RL   Mol. Biol. Cell 12:2907-2920(2001).
RN   [5]
RP   DISRUPTION PHENOTYPE.
RX   PubMed=19884248; DOI=10.1093/pcp/pcp137;
RA   Niihama M., Takemoto N., Hashiguchi Y., Tasaka M., Morita M.T.;
RT   "ZIP genes encode proteins involved in membrane trafficking of the TGN-
RT   PVC/vacuoles.";
RL   Plant Cell Physiol. 50:2057-2068(2009).
RN   [6]
RP   COMPONENT OF THE AP-3 COMPLEX.
RX   PubMed=21670741; DOI=10.1038/cr.2011.99;
RA   Zwiewka M., Feraru E., Moeller B., Hwang I., Feraru M.I., Kleine-Vehn J.,
RA   Weijers D., Friml J.;
RT   "The AP-3 adaptor complex is required for vacuolar function in
RT   Arabidopsis.";
RL   Cell Res. 21:1711-1722(2011).
CC   -!- FUNCTION: Part of the AP-3 complex, an adaptor-related complex which
CC       seems to be clathrin-associated. The complex is associated with the
CC       Golgi region as well as more peripheral structures. It facilitates the
CC       budding of vesicles from the Golgi membrane and may be directly
CC       involved in trafficking to the vacuole. It also function in maintaining
CC       the identity of lytic vacuoles and in regulating the transition between
CC       storage and lytic vacuoles (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Adaptor protein complex 3 (AP-3) is a heterotetramer composed
CC       of two large adaptins (delta-type subunit and beta-type subunit), a
CC       medium adaptin (mu-type subunit) and a small adaptin (sigma-type
CC       subunit).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Golgi apparatus.
CC       Cytoplasmic vesicle membrane {ECO:0000250}; Peripheral membrane protein
CC       {ECO:0000250}; Cytoplasmic side {ECO:0000250}. Note=Component of the
CC       coat surrounding the cytoplasmic face of coated vesicles located at the
CC       Golgi complex. {ECO:0000250}.
CC   -!- DISRUPTION PHENOTYPE: No obvious phenotype.
CC       {ECO:0000269|PubMed:19884248}.
CC   -!- SIMILARITY: Belongs to the adaptor complexes small subunit family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAB42915.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AL049862; CAB42915.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; CP002686; AEE78719.1; -; Genomic_DNA.
DR   EMBL; AY065287; AAL38763.1; -; mRNA.
DR   EMBL; AY096709; AAM20343.1; -; mRNA.
DR   PIR; T08407; T08407.
DR   RefSeq; NP_001327312.1; NM_001339486.1.
DR   RefSeq; NP_190655.2; NM_114946.2.
DR   AlphaFoldDB; Q8VZ37; -.
DR   SMR; Q8VZ37; -.
DR   BioGRID; 9568; 1.
DR   IntAct; Q8VZ37; 1.
DR   STRING; 3702.AT3G50860.1; -.
DR   PaxDb; Q8VZ37; -.
DR   ProteomicsDB; 244485; -.
DR   EnsemblPlants; AT3G50860.1; AT3G50860.1; AT3G50860.
DR   GeneID; 824250; -.
DR   Gramene; AT3G50860.1; AT3G50860.1; AT3G50860.
DR   KEGG; ath:AT3G50860; -.
DR   Araport; AT3G50860; -.
DR   TAIR; locus:2077922; AT3G50860.
DR   eggNOG; KOG0936; Eukaryota.
DR   HOGENOM; CLU_061221_2_2_1; -.
DR   InParanoid; Q8VZ37; -.
DR   OrthoDB; 1458249at2759; -.
DR   PhylomeDB; Q8VZ37; -.
DR   PRO; PR:Q8VZ37; -.
DR   Proteomes; UP000006548; Chromosome 3.
DR   ExpressionAtlas; Q8VZ37; baseline and differential.
DR   Genevisible; Q8VZ37; AT.
DR   GO; GO:0030123; C:AP-3 adaptor complex; IEA:InterPro.
DR   GO; GO:0030659; C:cytoplasmic vesicle membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005794; C:Golgi apparatus; IEA:UniProtKB-SubCell.
DR   GO; GO:0043231; C:intracellular membrane-bounded organelle; IBA:GO_Central.
DR   GO; GO:0006896; P:Golgi to vacuole transport; IEA:InterPro.
DR   GO; GO:0006886; P:intracellular protein transport; IEA:InterPro.
DR   GO; GO:0016192; P:vesicle-mediated transport; IBA:GO_Central.
DR   CDD; cd14834; AP3_sigma; 1.
DR   InterPro; IPR016635; AP_complex_ssu.
DR   InterPro; IPR022775; AP_mu_sigma_su.
DR   InterPro; IPR027155; APS3.
DR   InterPro; IPR000804; Clathrin_sm-chain_CS.
DR   InterPro; IPR011012; Longin-like_dom_sf.
DR   PANTHER; PTHR11753; PTHR11753; 1.
DR   Pfam; PF01217; Clat_adaptor_s; 1.
DR   PIRSF; PIRSF015588; AP_complex_sigma; 1.
DR   SUPFAM; SSF64356; SSF64356; 1.
DR   PROSITE; PS00989; CLAT_ADAPTOR_S; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Cytoplasmic vesicle; Golgi apparatus; Membrane;
KW   Protein transport; Reference proteome; Transport.
FT   CHAIN           1..166
FT                   /note="AP-3 complex subunit sigma"
FT                   /id="PRO_0000397859"
SQ   SEQUENCE   166 AA;  18729 MW;  D7C5B317CB2C6750 CRC64;
     MIKAVMMMNT QGKPRLAKFY DYLPVEKQQE LIRGVFSVLC SRPENVSNFL EIESLFGPDS
     RLVYKHYATL YFVLVFDGSE NELAMLDLIQ VLVETLDKCF SNVCELDIVF NYSKMHAVLD
     EIVFGGQVLE TSSAEVMKAV EEISKLEAAS NSISLVPKSV SGWRGR