AP4AT_MOUSE
ID AP4AT_MOUSE Reviewed; 573 AA.
AC Q3U3N6; Q8C1H2;
DT 15-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2005, sequence version 1.
DT 03-AUG-2022, entry version 115.
DE RecName: Full=AP-4 complex accessory subunit Tepsin {ECO:0000305};
DE AltName: Full=ENTH domain-containing protein 2 {ECO:0000312|MGI:MGI:1926027};
GN Name=Tepsin {ECO:0000312|MGI:MGI:1926027};
GN Synonyms=Enthd2 {ECO:0000312|MGI:MGI:1926027};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J, and NOD; TISSUE=Dendritic cell, and Embryonic stem cell;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
CC -!- FUNCTION: Associates with the adapter-like complex 4 (AP-4) and may
CC therefore play a role in vesicular trafficking of proteins at the
CC trans-Golgi network. {ECO:0000250|UniProtKB:Q96N21}.
CC -!- SUBUNIT: Interacts with AP4B1 and AP4E1; the interaction is direct and
CC mediates the association of TEPSIN with the adapter-like complex 4 (AP-
CC 4), a heterotetramer composed of AP4B1, AP4E1, AP4M1 and AP4S1.
CC {ECO:0000250|UniProtKB:Q96N21}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus, trans-Golgi network membrane
CC {ECO:0000250|UniProtKB:Q96N21}; Peripheral membrane protein
CC {ECO:0000250|UniProtKB:Q96N21}. Cytoplasmic vesicle
CC {ECO:0000250|UniProtKB:Q96N21}. Cytoplasm, cytosol
CC {ECO:0000250|UniProtKB:Q96N21}. Note=Extensively colocalizes with AP-4
CC which mediates the recruitment of TEPSIN to the trans-Golgi network.
CC {ECO:0000250|UniProtKB:Q96N21}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC25578.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AK019105; BAC25578.1; ALT_FRAME; mRNA.
DR EMBL; AK154660; BAE32749.1; -; mRNA.
DR EMBL; AL807824; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR CCDS; CCDS36387.1; -.
DR RefSeq; NP_898960.2; NM_183137.2.
DR AlphaFoldDB; Q3U3N6; -.
DR SMR; Q3U3N6; -.
DR STRING; 10090.ENSMUSP00000091428; -.
DR iPTMnet; Q3U3N6; -.
DR PhosphoSitePlus; Q3U3N6; -.
DR jPOST; Q3U3N6; -.
DR MaxQB; Q3U3N6; -.
DR PaxDb; Q3U3N6; -.
DR PRIDE; Q3U3N6; -.
DR ProteomicsDB; 281897; -.
DR Antibodypedia; 53274; 13 antibodies from 6 providers.
DR Ensembl; ENSMUST00000093901; ENSMUSP00000091428; ENSMUSG00000025377.
DR GeneID; 78777; -.
DR KEGG; mmu:78777; -.
DR UCSC; uc007mrq.1; mouse.
DR CTD; 146705; -.
DR MGI; MGI:1926027; Tepsin.
DR VEuPathDB; HostDB:ENSMUSG00000025377; -.
DR eggNOG; ENOG502QV38; Eukaryota.
DR GeneTree; ENSGT00390000015076; -.
DR InParanoid; Q3U3N6; -.
DR OMA; SHCLLEY; -.
DR OrthoDB; 433095at2759; -.
DR PhylomeDB; Q3U3N6; -.
DR TreeFam; TF331354; -.
DR BioGRID-ORCS; 78777; 0 hits in 70 CRISPR screens.
DR ChiTaRS; Enthd2; mouse.
DR PRO; PR:Q3U3N6; -.
DR Proteomes; UP000000589; Chromosome 11.
DR RNAct; Q3U3N6; protein.
DR Bgee; ENSMUSG00000025377; Expressed in granulocyte and 91 other tissues.
DR ExpressionAtlas; Q3U3N6; baseline and differential.
DR Genevisible; Q3U3N6; MM.
DR GO; GO:0030124; C:AP-4 adaptor complex; ISO:MGI.
DR GO; GO:0030662; C:coated vesicle membrane; ISS:UniProtKB.
DR GO; GO:0005737; C:cytoplasm; ISO:MGI.
DR GO; GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
DR GO; GO:0031312; C:extrinsic component of organelle membrane; ISO:MGI.
DR GO; GO:0005794; C:Golgi apparatus; ISO:MGI.
DR GO; GO:0031965; C:nuclear membrane; ISO:MGI.
DR GO; GO:0016607; C:nuclear speck; ISO:MGI.
DR GO; GO:0032588; C:trans-Golgi network membrane; ISO:MGI.
DR CDD; cd03572; ENTH_like_Tepsin; 1.
DR Gene3D; 1.25.40.90; -; 1.
DR InterPro; IPR013809; ENTH.
DR InterPro; IPR035802; ENTH/VHS_tepsin.
DR InterPro; IPR008942; ENTH_VHS.
DR InterPro; IPR039273; TEPSIN.
DR PANTHER; PTHR21514; PTHR21514; 1.
DR Pfam; PF01417; ENTH; 1.
DR SUPFAM; SSF48464; SSF48464; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; Cytoplasmic vesicle; Golgi apparatus; Membrane; Phosphoprotein;
KW Reference proteome.
FT CHAIN 1..573
FT /note="AP-4 complex accessory subunit Tepsin"
FT /id="PRO_0000286679"
FT DOMAIN 8..141
FT /note="ENTH"
FT REGION 136..155
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 194..311
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 497..526
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 526..536
FT /note="Interaction with AP4B1"
FT /evidence="ECO:0000250|UniProtKB:Q96N21"
FT REGION 563..573
FT /note="Interaction with AP4E1"
FT /evidence="ECO:0000250|UniProtKB:Q96N21"
FT COMPBIAS 137..151
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 257..292
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 400
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q96N21"
FT CONFLICT 73
FT /note="L -> V (in Ref. 1; BAC25578)"
FT /evidence="ECO:0000305"
FT CONFLICT 324
FT /note="P -> Q (in Ref. 1; BAC25578)"
FT /evidence="ECO:0000305"
FT CONFLICT 462
FT /note="S -> R (in Ref. 1; BAC25578)"
FT /evidence="ECO:0000305"
FT CONFLICT 483..485
FT /note="SAL -> CAV (in Ref. 1; BAC25578)"
FT /evidence="ECO:0000305"
FT CONFLICT 537
FT /note="A -> G (in Ref. 1; BAC25578)"
FT /evidence="ECO:0000305"
FT CONFLICT 560
FT /note="A -> G (in Ref. 1; BAC25578)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 573 AA; 60761 MW; 4E6FD6D9A2E3B887 CRC64;
MAAVPPLRDR LSFLHRLPIL LKGTSDDSIP CPGYLFEEIA KISHESLGSS QCLLEYLLNR
LDSSSGHVKL KVLKILLYLC GHGSSSFLLI LRRNSALIQE ATAFSGPPDP LHGNSLYQKV
RAAAQDLGST LFSDAVPQPP SQPPQIPPPA GMGAQARPLS ALQGFGYTKE SSRTGSAGET
FLSTIQRAAE VVANAVRPGP DNPCTKGPLP YGDSYQPAVT PSASHTHPNP GNLLPGAILG
ARAVRHQPGQ AGGGWDELDS SPSSQNSSCT SNLSRASDSG SRSGSDSHSG TSREPGDLAE
RAEATPPNDC QQELNLVRTV TQGPRVFLSR EETQHFIKEC GLLNCEAVLE LLLRQLVGTS
ECEQMRALCA IASFGSADLL PQEHVLLLCR QQLQELGAGS PGPVTNKATK ILRHFEASCG
QQLPTLRLCA QPNSAAAPVG PADLLTSPVP APGSQVFLQP LSSATVVPRS PVLFPSPNTL
PPSALEEPSE VRTQLVCSSE QGTESEQRLE NTDTPEDSSS PLPWSPNSLF AGMELVACPR
LPCHSSQDLQ TDLQKVTTEA PVSEPSAFAF LNM
