AP4E_ARATH
ID AP4E_ARATH Reviewed; 938 AA.
AC Q8L7A9; Q9C6W3;
DT 05-OCT-2010, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 25-MAY-2022, entry version 116.
DE RecName: Full=AP-4 complex subunit epsilon;
DE AltName: Full=AP-4 adaptor complex subunit epsilon;
DE AltName: Full=Adaptor-related protein complex 4 subunit epsilon;
DE AltName: Full=Epsilon subunit of AP-4;
DE AltName: Full=Epsilon-adaptin;
GN OrderedLocusNames=At1g31730; ORFNames=F27M3.7;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP GENE FAMILY, AND REVIEW.
RX PubMed=11598180; DOI=10.1091/mbc.12.10.2907;
RA Boehm M., Bonifacino J.S.;
RT "Adaptins: the final recount.";
RL Mol. Biol. Cell 12:2907-2920(2001).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19376835; DOI=10.1104/pp.109.138677;
RA Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A.,
RA Grossmann J., Gruissem W., Baginsky S.;
RT "Large-scale Arabidopsis phosphoproteome profiling reveals novel
RT chloroplast kinase substrates and phosphorylation networks.";
RL Plant Physiol. 150:889-903(2009).
CC -!- FUNCTION: Subunit of novel type of clathrin- or non-clathrin-associated
CC protein coat involved in targeting proteins from the trans-Golgi
CC network (TGN) to the endosomal-lysosomal system. {ECO:0000250}.
CC -!- SUBUNIT: Adaptor protein complex 4 (AP-4) is a heterotetramer composed
CC of two large adaptins (epsilon-type subunit and beta-type subunit), a
CC medium adaptin (mu-type subunit) and a small adaptin (sigma-type
CC subunit). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus, trans-Golgi network
CC {ECO:0000250}. Membrane, coated pit {ECO:0000250}. Note=Associated with
CC the trans-Golgi network. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the adaptor complexes large subunit family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAG60138.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC074360; AAG60138.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE31386.1; -; Genomic_DNA.
DR EMBL; AY136369; AAM97035.1; -; mRNA.
DR EMBL; BT000268; AAN15587.1; -; mRNA.
DR RefSeq; NP_174454.2; NM_102908.3.
DR AlphaFoldDB; Q8L7A9; -.
DR SMR; Q8L7A9; -.
DR BioGRID; 25294; 1.
DR STRING; 3702.AT1G31730.1; -.
DR iPTMnet; Q8L7A9; -.
DR PaxDb; Q8L7A9; -.
DR PRIDE; Q8L7A9; -.
DR ProteomicsDB; 244990; -.
DR ABCD; Q8L7A9; 2 sequenced antibodies.
DR EnsemblPlants; AT1G31730.1; AT1G31730.1; AT1G31730.
DR GeneID; 840060; -.
DR Gramene; AT1G31730.1; AT1G31730.1; AT1G31730.
DR KEGG; ath:AT1G31730; -.
DR Araport; AT1G31730; -.
DR TAIR; locus:2028586; AT1G31730.
DR eggNOG; KOG1062; Eukaryota.
DR HOGENOM; CLU_003824_3_2_1; -.
DR InParanoid; Q8L7A9; -.
DR OMA; VLMAMTK; -.
DR OrthoDB; 250202at2759; -.
DR PhylomeDB; Q8L7A9; -.
DR PRO; PR:Q8L7A9; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q8L7A9; baseline and differential.
DR Genevisible; Q8L7A9; AT.
DR GO; GO:0030124; C:AP-4 adaptor complex; IPI:TAIR.
DR GO; GO:0005905; C:clathrin-coated pit; IEA:UniProtKB-KW.
DR GO; GO:0005794; C:Golgi apparatus; IEA:UniProtKB-SubCell.
DR GO; GO:0009506; C:plasmodesma; HDA:TAIR.
DR GO; GO:0140312; F:cargo adaptor activity; IBA:GO_Central.
DR GO; GO:0006886; P:intracellular protein transport; IEA:InterPro.
DR GO; GO:0006898; P:receptor-mediated endocytosis; IBA:GO_Central.
DR Gene3D; 1.25.10.10; -; 1.
DR InterPro; IPR017109; AP4_complex_esu.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR002553; Clathrin/coatomer_adapt-like_N.
DR Pfam; PF01602; Adaptin_N; 1.
DR PIRSF; PIRSF037097; AP4_complex_epsilon; 1.
DR SUPFAM; SSF48371; SSF48371; 1.
PE 1: Evidence at protein level;
KW Coated pit; Golgi apparatus; Membrane; Protein transport;
KW Reference proteome; Repeat; Transport.
FT CHAIN 1..938
FT /note="AP-4 complex subunit epsilon"
FT /id="PRO_0000397855"
FT REPEAT 118..153
FT /note="HEAT 1"
FT REPEAT 154..190
FT /note="HEAT 2"
FT REPEAT 192..227
FT /note="HEAT 3"
FT REPEAT 234..272
FT /note="HEAT 4"
FT REPEAT 321..358
FT /note="HEAT 5"
FT REPEAT 359..395
FT /note="HEAT 6"
FT REPEAT 397..431
FT /note="HEAT 7"
FT REPEAT 454..495
FT /note="HEAT 8"
FT REPEAT 517..556
FT /note="HEAT 9"
FT REPEAT 562..601
FT /note="HEAT 10"
FT REPEAT 874..911
FT /note="HEAT 11"
FT REGION 690..712
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 725..867
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 880..912
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 919..938
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 695..709
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 726..764
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 880..907
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 921..938
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 938 AA; 103735 MW; C9ECEA36AB09EDF8 CRC64;
MEQLKTIGRE LAMGSQGGFG QSKEFLDLVK SIGEARSKAE EDRIVLSEVD ILKRRLLEPD
IPKRKMKEYI IRLVYIEMLG HDASFGYIYA VKMTHDDNLL LKRTGYLAVT LFLNEDHDLI
ILIVNTIQKD LRSDNYLVVC AALNAICRLI NEETIPAVLP QVVELLNHQK EAVRKKAIMA
LHRFHRKSPS SVSHLVSNFR KRLCDNDPGV MGATLCPLFD LISEDVNSYK DLVSSFVSIL
KQVTERRLPK SYDYHQMPAP FIQIKLLKIM ALLGSGDKNA SDIMSMVLGD LFRKCDSSTN
IGNAILYECI RCISCILPNP KLLEAAADAI SKFLKSDSHN LKYMGIDGLG RLIKISPDIA
EQHQLAVIDC LEDPDDTLKR KTFELLYKMT KSSNVEVIVD RMIDYMISIN DNHYKTEIAS
RCVELAEQFA PSNQWFIQIM NKVFEHAGDL VNIKVAHNLM RLIAEGFGED DDDADSKLRL
SAVESYLQLI SEPKLPSLFL QVISWVLGEY GTADGKYSAS YISGKLCDVA DAYSSDETVK
GYAVSALMKI YAFEIASGRK VDVLPECQSL IEELLASHST DLQQRAYELQ ALLALDARAV
ETILPLDASC EDIEVDKDLS FLNGYIQQAI ESGAQPYISE RERSGMFETT DYHPQDHHEV
PTHALRFEAY ELPKPSVPPQ ASNELVPVPE PSYYSESHQP ISTSLVSERE SSEIKLRLDG
VKQKWGRPSY QSTTAASSTT PQAANGISTH SDAGVGSSSS KPRSSYEPKK PEIDPEKQRL
AASLFGGSSS RTDKRSSSGG HKPAKGTANK TATVPKENQT PVQPPPDLLD FGEPTATTAT
AMDPFKELEG LMDSSSQDGG SSDVMGLYSD AAPVTTTTSV DSLLSELSDS SKGNSRTYQP
QTSKGPNTKE ALEKDALVRQ MGVNPTSQNP TLFKDLLG
