ID   AP4E_DICDI              Reviewed;        1080 AA.
AC   Q54VE0;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   25-MAY-2022, entry version 89.
DE   RecName: Full=AP-4 complex subunit epsilon {ECO:0000305};
DE   AltName: Full=AP-4 adaptor complex subunit epsilon;
DE   AltName: Full=Adaptor-related protein complex 4 subunit epsilon;
DE   AltName: Full=Epsilon subunit of AP-4;
DE   AltName: Full=Epsilon-adaptin;
GN   Name=ap4e1 {ECO:0000312|dictyBase:DDB_G0280427};
GN   ORFNames=DDB_G0280427 {ECO:0000312|dictyBase:DDB_G0280427};
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- FUNCTION: Probable component of an adaptor protein complex. Adaptor
CC       protein complexes are vesicle coat components involved both in vesicle
CC       formation and cargo selection. They control the vesicular transport of
CC       proteins in different trafficking pathways.
CC       {ECO:0000250|UniProtKB:Q9UPM8}.
CC   -!- SUBUNIT: May be part of the adaptor protein complex 4 (AP-4), a
CC       heterotetramer composed of two large adaptins (epsilon-type subunitand
CC       beta-type subunit), a medium adaptin (mu-type subunit) and a small
CC       adaptin (sigma-type). {ECO:0000250|UniProtKB:Q9UPM8}.
CC   -!- SUBCELLULAR LOCATION: Golgi apparatus, trans-Golgi network membrane
CC       {ECO:0000250|UniProtKB:Q9UPM8}; Peripheral membrane protein
CC       {ECO:0000250|UniProtKB:Q9UPM8}.
CC   -!- SIMILARITY: Belongs to the adaptor complexes large subunit family.
CC       {ECO:0000305}.
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DR   EMBL; AAFI02000036; EAL67192.1; -; Genomic_DNA.
DR   RefSeq; XP_641169.1; XM_636077.1.
DR   AlphaFoldDB; Q54VE0; -.
DR   SMR; Q54VE0; -.
DR   STRING; 44689.DDB0237723; -.
DR   PaxDb; Q54VE0; -.
DR   PRIDE; Q54VE0; -.
DR   EnsemblProtists; EAL67192; EAL67192; DDB_G0280427.
DR   GeneID; 8622549; -.
DR   KEGG; ddi:DDB_G0280427; -.
DR   dictyBase; DDB_G0280427; ap4e1.
DR   eggNOG; KOG1062; Eukaryota.
DR   HOGENOM; CLU_286275_0_0_1; -.
DR   InParanoid; Q54VE0; -.
DR   OMA; IMAVNSI; -.
DR   PhylomeDB; Q54VE0; -.
DR   Reactome; R-DDI-432720; Lysosome Vesicle Biogenesis.
DR   PRO; PR:Q54VE0; -.
DR   Proteomes; UP000002195; Chromosome 3.
DR   GO; GO:0030124; C:AP-4 adaptor complex; IBA:GO_Central.
DR   GO; GO:0005794; C:Golgi apparatus; IEA:UniProtKB-SubCell.
DR   GO; GO:0140312; F:cargo adaptor activity; IBA:GO_Central.
DR   GO; GO:0006886; P:intracellular protein transport; IEA:InterPro.
DR   GO; GO:0006898; P:receptor-mediated endocytosis; IBA:GO_Central.
DR   Gene3D; 1.25.10.10; -; 1.
DR   InterPro; IPR011989; ARM-like.
DR   InterPro; IPR016024; ARM-type_fold.
DR   InterPro; IPR002553; Clathrin/coatomer_adapt-like_N.
DR   Pfam; PF01602; Adaptin_N; 1.
DR   SUPFAM; SSF48371; SSF48371; 1.
PE   3: Inferred from homology;
KW   Golgi apparatus; Membrane; Protein transport; Reference proteome; Repeat;
KW   Transport.
FT   CHAIN           1..1080
FT                   /note="AP-4 complex subunit epsilon"
FT                   /id="PRO_0000328364"
FT   REPEAT          161..198
FT                   /note="HEAT 1"
FT   REPEAT          201..238
FT                   /note="HEAT 2"
FT   REPEAT          369..405
FT                   /note="HEAT 3"
FT   REPEAT          406..443
FT                   /note="HEAT 4"
FT   REPEAT          445..479
FT                   /note="HEAT 5"
FT   REGION          711..782
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          801..920
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          933..973
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          996..1027
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        714..729
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        743..782
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        801..853
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        877..914
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1080 AA;  123904 MW;  5B7385167F14A476 CRC64;
     MNSSALNLIQ FLESSKIQVP GGLKSVKGIL DFDFYDLVKN IGESTSREEE VHIIQNEIIK
     LKSCFSKEQS KDKKRECLIR MIYCHMLGYD VPFGHIQALN MTQDSEILNK RTGYLTLSLC
     LPERHELLIM AVNSILKGLN SSNYLEVCSA LTAMCKLIDN DTIPAFLQKV LQLLNHQKPI
     VRKKSVTVLH RFYRLVGDSF LDDDQIIDKL RQSLCDRDPS VMSASICIFL DISEKHSTLK
     DNNNQINNNN NNNNSNQIKK KNNEEINRSK NLISMLKELV PSFVGILKQV AEGRLPNSYI
     YHGIHHPWLQ INLLKLLSNL GYQDKDSSNH MYTVLLFTMQ QSQKFKNNVG FAILYETIKT
     LTLIHPNLQL IEQCSKNIAI FLKGKHHNLR YFGIKALASI VKVSPKLVLP YQVEVIESLE
     SPDETLKRKS FDLLYKMTNQ TNVVPVCSKL IEQLVLSKDQ NFKSELISQI TNIAEKYSPN
     DIWYIDTIST VLSILPNENN KDNNNNNNNN NNNNNNYQFA YNLIRLVSEE DDIKVKQHIS
     EIYLNNIMIS NEQQQQQQQE NQNNLQQFSD IYIKIMSWVI SEYSNLIVSN NGVIESDIIS
     YLCDLLEKDY QGETKSWIII GIGKLVAQLG KSLPMLELMT KKFKSSKSLI CQQRSSELNE
     ILKNPKSMSL ILPLDAYCED IDFNKIFNKF NDYSNKVGGK QYIPYEKRKN TPLVDISDGS
     SSHSNEKGLN FQYPPPPDPF NPHHNQQQLY PVHQHQPPSN HQQQQQQQQQ QQQQQLQLFP
     QQPQQPQQLL LLGEDDTINL NNNSLVPVNN DQQLQPHQHQ QQQQNQQQQQ QNQQQQQQQN
     PQYPNNQLVT VPDQNAPKPI GLPKPSKVLW TKKGFVGNKQ TPPQNATPQQ QQQQQQQQTQ
     NTLSQQQIQK HQQTHNNIDP EKEKLAKQLF GGFSENNNRN ENSNNTDNNQ NNVGITSKLQ
     NKKSNNENNI NNNKKESYLE ELIDLSPNLI SNINITNNSN NNNNNNNNNN NNNNNNNNNN
     NNNNNNLLLV DVEGDEQKEV KNSSCSSGNS ELINLNLDEN TTKNINGNLL GDDLEELNKN