ID   APAF_DANRE              Reviewed;        1261 AA.
AC   Q9I9H8;
DT   23-JAN-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   03-AUG-2022, entry version 126.
DE   RecName: Full=Apoptotic protease-activating factor 1;
DE            Short=APAF-1;
GN   Name=apaf1;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=10917738; DOI=10.1038/sj.cdd.4400679;
RA   Inohara N., Nunez G.;
RT   "Genes with homology to mammalian apoptosis regulators identified in
RT   zebrafish.";
RL   Cell Death Differ. 7:509-510(2000).
CC   -!- FUNCTION: Oligomeric Apaf-1 mediates the cytochrome c-dependent
CC       autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the
CC       activation of caspase-3 and apoptosis. This activation requires ATP (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Monomer. Oligomerizes upon binding of cytochrome c and dATP
CC       (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
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DR   EMBL; AF251502; AAF67189.1; -; mRNA.
DR   RefSeq; NP_571683.1; NM_131608.1.
DR   AlphaFoldDB; Q9I9H8; -.
DR   SMR; Q9I9H8; -.
DR   STRING; 7955.ENSDARP00000105810; -.
DR   PaxDb; Q9I9H8; -.
DR   PRIDE; Q9I9H8; -.
DR   GeneID; 58131; -.
DR   KEGG; dre:58131; -.
DR   CTD; 317; -.
DR   ZFIN; ZDB-GENE-000616-4; apaf1.
DR   eggNOG; KOG4155; Eukaryota.
DR   eggNOG; KOG4658; Eukaryota.
DR   InParanoid; Q9I9H8; -.
DR   PhylomeDB; Q9I9H8; -.
DR   Reactome; R-DRE-111458; Formation of apoptosome.
DR   Reactome; R-DRE-111459; Activation of caspases through apoptosome-mediated cleavage.
DR   Reactome; R-DRE-6798695; Neutrophil degranulation.
DR   Reactome; R-DRE-9627069; Regulation of the apoptosome activity.
DR   PRO; PR:Q9I9H8; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Unplaced.
DR   GO; GO:0043293; C:apoptosome; IEA:InterPro.
DR   GO; GO:0043531; F:ADP binding; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0048856; P:anatomical structure development; IEA:UniProt.
DR   GO; GO:0097190; P:apoptotic signaling pathway; IEA:InterPro.
DR   GO; GO:0042981; P:regulation of apoptotic process; IEA:InterPro.
DR   Gene3D; 1.10.10.10; -; 1.
DR   Gene3D; 1.10.533.10; -; 1.
DR   Gene3D; 1.10.8.430; -; 1.
DR   Gene3D; 2.130.10.10; -; 2.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR017251; Apaf-1.
DR   InterPro; IPR041452; APAF1_C.
DR   InterPro; IPR042197; Apaf_helical.
DR   InterPro; IPR001315; CARD.
DR   InterPro; IPR011029; DEATH-like_dom_sf.
DR   InterPro; IPR020472; G-protein_beta_WD-40_rep.
DR   InterPro; IPR002182; NB-ARC.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   InterPro; IPR001680; WD40_repeat.
DR   InterPro; IPR019775; WD40_repeat_CS.
DR   InterPro; IPR036322; WD40_repeat_dom_sf.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   Pfam; PF17908; APAF1_C; 1.
DR   Pfam; PF00619; CARD; 1.
DR   Pfam; PF00931; NB-ARC; 1.
DR   Pfam; PF00400; WD40; 7.
DR   PIRSF; PIRSF037646; Apop_pept_activating-1; 1.
DR   PRINTS; PR00320; GPROTEINBRPT.
DR   SMART; SM00320; WD40; 14.
DR   SUPFAM; SSF47986; SSF47986; 1.
DR   SUPFAM; SSF50978; SSF50978; 2.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS50209; CARD; 1.
DR   PROSITE; PS00678; WD_REPEATS_1; 2.
DR   PROSITE; PS50082; WD_REPEATS_2; 7.
DR   PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE   2: Evidence at transcript level;
KW   Apoptosis; ATP-binding; Cytoplasm; Nucleotide-binding; Reference proteome;
KW   Repeat; WD repeat.
FT   CHAIN           1..1261
FT                   /note="Apoptotic protease-activating factor 1"
FT                   /id="PRO_0000050847"
FT   DOMAIN          1..90
FT                   /note="CARD"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00046"
FT   DOMAIN          106..417
FT                   /note="NB-ARC"
FT   REPEAT          615..654
FT                   /note="WD 1"
FT   REPEAT          657..696
FT                   /note="WD 2"
FT   REPEAT          700..743
FT                   /note="WD 3"
FT   REPEAT          746..785
FT                   /note="WD 4"
FT   REPEAT          798..836
FT                   /note="WD 5"
FT   REPEAT          840..879
FT                   /note="WD 6"
FT   REPEAT          882..921
FT                   /note="WD 7"
FT   REPEAT          964..1003
FT                   /note="WD 8"
FT   REPEAT          1006..1045
FT                   /note="WD 9"
FT   REPEAT          1047..1088
FT                   /note="WD 10"
FT   REPEAT          1091..1130
FT                   /note="WD 11"
FT   REPEAT          1133..1172
FT                   /note="WD 12"
FT   REPEAT          1184..1223
FT                   /note="WD 13"
FT   BINDING         154..161
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   1261 AA;  142934 MW;  F80CC9CB41764F1C CRC64;
     MEERARSRLL RSKATLEQDI KASYLMDHMI SDGVLTNDEE AKVLSKATRK EQAVALLETL
     LRKDNRAYIS FYNALIRESY GDLASLLHSD LPLLSPEGEK SFADGVSPSV QAILSVGGVP
     QRPVVFVSRP PLLNLIREML YQLRDTPGWV TVFGMAGSGK SVMAAEVVRD RSLIKECFPD
     GVHWLSVGQC ERADLLVRMQ SLCFRLEQCQ SSDTSQRPPS TVEEAKERLR FLMLRRFPRS
     LLILDDVWDS SSLRSFDIQC RVLLTTRNRA LTDSVSGVRY EVPVENGLDE EKALEILALY
     VNGKMHKLPE QARSIVSECK GSPLVVSLIG ALLREFPDRW SYYLRQLQQK QFKRIRKSSS
     YDYEALDQAM DASLQVLEAE HQELYRDLSV MQKDIKVPAK VLSVLWGLEL EEVEDVLQEF
     VNKSLLFRDC NQRPYRYYLH DLQLDFLAEQ NRDQIAELHK KMVRQYQRFY SKRPPDSADK
     DSLYWYQFIP YHMAKAGLSK ELYSLMFSLD WVKEKARIMG SAHLINDYVE YGEILDKENS
     EVRVQFQEFL SLNGHHLEQR PFPDVVQLAL SQPDRSEVYR QALMQAQKRA SRGQIYLNWV
     NKNIEEGLSR LVMHPHQGAV YYACFSKDGS KIASCGASKA LRVFKSTSGE KLLELQAHEE
     DVLCCAFSPD DRHIATCASD RKVKLWNVER GVLIREFEVE HEEQINHCQF TNTGRRVLLA
     TCSNDKFTNT RLWNPNKKTS QNTMFGHMEP VNHCCFSPND LYLATSSSDG SLKLFEVSSA
     NEWKSIDVDS FFPESDEEIK AMVKCSTWSA DGSQIICAAR NTVFVFDVET SDLLLKLKTS
     RLSTIQFCHA CPNSSLLAVA LSHYTVELWN FESSKKKAEC SGHLSWVHCV QFSPDGSLLL
     SSSDDQTIRL WETDRVHTSS AVALKRDTDV LSSHSDATII APDSSNRLQV LSGSTGAVVL
     ESEELSSRIR CSCISRNAAF VALGSEDGTV QVIEVPSSKA SVKLSGHTKT VHHCQFTDDC
     EILITSSEDS TIRVWKWRTG ECMVLQGHME PVRKFHLLSS SSSPHLFSWS FDGTVKVWDL
     TRGQMLQDLV CHEGAVLSCD VSSDGRLFAT TSANRTAKVW SSASWKMLFL LEGHKDCVRS
     CRFSWDNKRL ATGDDNGEIR LWSMLDGALL KICPRDTKDS MNSYHAGWVT DLHFSPDNRV
     LVSTAGYIKW WSVESGEALQ TFYTMGGNLK KIHVSPDFST FITVDSIGIL YILKRLEGEG
     T