ID   HSP1_PIG                Reviewed;          50 AA.
AC   P04101;
DT   01-NOV-1986, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 3.
DT   03-AUG-2022, entry version 101.
DE   RecName: Full=Sperm protamine P1;
DE   AltName: Full=Cysteine-rich protamine;
GN   Name=PRM1; Synonyms=PRM-1;
OS   Sus scrofa (Pig).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Suina; Suidae; Sus.
OX   NCBI_TaxID=9823;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Testis;
RX   PubMed=3211757; DOI=10.1093/nar/16.24.11826;
RA   Maier W.-M., Adham I.M., Klemm U.;
RT   "The nucleotide sequence of a boar protamine 1 cDNA.";
RL   Nucleic Acids Res. 16:11826-11826(1988).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1627265; DOI=10.1515/bchm3.1992.373.1.261;
RA   Keime S., Heitland K., Kumm S., Schloesser M., Hroch N., Holtz W.,
RA   Engel W.;
RT   "Characterization of four genes encoding basic proteins of the porcine
RT   spermatid nucleus and close linkage of three of them.";
RL   Biol. Chem. Hoppe-Seyler 373:261-270(1992).
RN   [3]
RP   NUCLEOTIDE SEQUENCE.
RC   TISSUE=Sperm;
RX   PubMed=8240358; DOI=10.1006/bbrc.1993.2324;
RA   Pirhonen A., Jaaskelainen S., Linnala-Kankkunen A., Maenpaa P.H.;
RT   "An aminoterminally extended P1-protamine variant in the boar.";
RL   Biochem. Biophys. Res. Commun. 196:831-836(1993).
RN   [4]
RP   PROTEIN SEQUENCE OF 2-50.
RA   Tobita T., Tsutsumi H., Kato A., Suzuki H., Nomoto M., Nakano M., Ando T.;
RT   "Complete amino acid sequence of boar protamine.";
RL   Biochim. Biophys. Acta 744:141-146(1983).
CC   -!- FUNCTION: Protamines substitute for histones in the chromatin of sperm
CC       during the haploid phase of spermatogenesis. They compact sperm DNA
CC       into a highly condensed, stable and inactive complex.
CC   -!- SUBUNIT: Cross-linked by interchain disulfide bonds around the DNA-
CC       helix.
CC   -!- SUBCELLULAR LOCATION: Nucleus. Chromosome.
CC   -!- TISSUE SPECIFICITY: Testis.
CC   -!- SIMILARITY: Belongs to the protamine P1 family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAB28721.2; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; X13381; CAA31758.1; -; mRNA.
DR   EMBL; M80678; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; S66702; AAB28721.2; ALT_INIT; Genomic_DNA.
DR   PIR; A02659; HSPG.
DR   PIR; S21672; S21672.
DR   RefSeq; NP_999418.1; NM_214253.1.
DR   AlphaFoldDB; P04101; -.
DR   STRING; 9823.ENSSSCP00000019687; -.
DR   PaxDb; P04101; -.
DR   PRIDE; P04101; -.
DR   Ensembl; ENSSSCT00015072053; ENSSSCP00015028898; ENSSSCG00015054098.
DR   Ensembl; ENSSSCT00025061938; ENSSSCP00025026300; ENSSSCG00025045596.
DR   Ensembl; ENSSSCT00030044903; ENSSSCP00030020180; ENSSSCG00030032482.
DR   Ensembl; ENSSSCT00035065987; ENSSSCP00035026776; ENSSSCG00035049493.
DR   Ensembl; ENSSSCT00040017446; ENSSSCP00040007124; ENSSSCG00040013140.
DR   Ensembl; ENSSSCT00045041379; ENSSSCP00045028726; ENSSSCG00045024279.
DR   Ensembl; ENSSSCT00050105533; ENSSSCP00050046437; ENSSSCG00050076776.
DR   Ensembl; ENSSSCT00055025718; ENSSSCP00055020439; ENSSSCG00055013080.
DR   Ensembl; ENSSSCT00060103087; ENSSSCP00060044958; ENSSSCG00060075310.
DR   Ensembl; ENSSSCT00065103977; ENSSSCP00065046025; ENSSSCG00065075354.
DR   Ensembl; ENSSSCT00070051566; ENSSSCP00070043608; ENSSSCG00070025800.
DR   GeneID; 397487; -.
DR   KEGG; ssc:397487; -.
DR   CTD; 5619; -.
DR   HOGENOM; CLU_214580_1_0_1; -.
DR   OMA; MARYICC; -.
DR   Proteomes; UP000008227; Unplaced.
DR   Proteomes; UP000314985; Chromosome 3.
DR   Genevisible; P04101; SS.
DR   GO; GO:0000786; C:nucleosome; IEA:UniProtKB-KW.
DR   GO; GO:0005634; C:nucleus; IDA:MGI.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0030261; P:chromosome condensation; IEA:UniProtKB-KW.
DR   GO; GO:0035092; P:sperm DNA condensation; IEA:InterPro.
DR   InterPro; IPR000221; Protamine_P1.
DR   Pfam; PF00260; Protamine_P1; 1.
DR   PROSITE; PS00048; PROTAMINE_P1; 1.
PE   1: Evidence at protein level;
KW   Chromosome; Developmental protein; Differentiation;
KW   Direct protein sequencing; Disulfide bond; DNA condensation; DNA-binding;
KW   Nucleosome core; Nucleus; Reference proteome; Spermatogenesis.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000269|Ref.4"
FT   CHAIN           2..50
FT                   /note="Sperm protamine P1"
FT                   /id="PRO_0000191531"
FT   DISULFID        6
FT                   /note="Interchain (with C-22)"
FT                   /evidence="ECO:0000250|UniProtKB:P02318"
FT   DISULFID        7..15
FT                   /evidence="ECO:0000250|UniProtKB:P02318"
FT   DISULFID        22
FT                   /note="Interchain (with C-6)"
FT                   /evidence="ECO:0000250|UniProtKB:P02318"
FT   DISULFID        38
FT                   /note="Interchain (with C-38)"
FT                   /evidence="ECO:0000250|UniProtKB:P02318"
FT   DISULFID        39..47
FT                   /evidence="ECO:0000250|UniProtKB:P02318"
FT   CONFLICT        35
FT                   /note="R -> RR (in Ref. 4; AA sequence)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   50 AA;  6559 MW;  D056D31B7AC89791 CRC64;
     MARYRCCRSH SRSRCRPRRR RCRRRRRRCC PRRRRAVCCR RYTVIRCRRC