ID   HSP71_CRYNH             Reviewed;         643 AA.
AC   J9VZ70;
DT   10-FEB-2021, integrated into UniProtKB/Swiss-Prot.
DT   28-NOV-2012, sequence version 1.
DT   03-AUG-2022, entry version 43.
DE   RecName: Full=Transcriptional coregulator SSA1 {ECO:0000303|PubMed:25972480};
GN   Name=SSA1 {ECO:0000303|PubMed:25972480};
GN   ORFNames=CNAG_01727 {ECO:0000312|EMBL:AFR97929.1};
OS   Cryptococcus neoformans var. grubii serotype A (strain H99 / ATCC 208821 /
OS   CBS 10515 / FGSC 9487) (Filobasidiella neoformans var. grubii).
OC   Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Tremellomycetes;
OC   Tremellales; Cryptococcaceae; Cryptococcus;
OC   Cryptococcus neoformans species complex.
OX   NCBI_TaxID=235443 {ECO:0000312|Proteomes:UP000010091};
RN   [1] {ECO:0000312|Proteomes:UP000010091}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=H99 / ATCC 208821 / CBS 10515 / FGSC 9487;
RX   PubMed=24743168; DOI=10.1371/journal.pgen.1004261;
RA   Janbon G., Ormerod K.L., Paulet D., Byrnes E.J. III, Yadav V.,
RA   Chatterjee G., Mullapudi N., Hon C.-C., Billmyre R.B., Brunel F.,
RA   Bahn Y.-S., Chen W., Chen Y., Chow E.W.L., Coppee J.-Y., Floyd-Averette A.,
RA   Gaillardin C., Gerik K.J., Goldberg J., Gonzalez-Hilarion S., Gujja S.,
RA   Hamlin J.L., Hsueh Y.-P., Ianiri G., Jones S., Kodira C.D., Kozubowski L.,
RA   Lam W., Marra M., Mesner L.D., Mieczkowski P.A., Moyrand F., Nielsen K.,
RA   Proux C., Rossignol T., Schein J.E., Sun S., Wollschlaeger C., Wood I.A.,
RA   Zeng Q., Neuveglise C., Newlon C.S., Perfect J.R., Lodge J.K., Idnurm A.,
RA   Stajich J.E., Kronstad J.W., Sanyal K., Heitman J., Fraser J.A.,
RA   Cuomo C.A., Dietrich F.S.;
RT   "Analysis of the genome and transcriptome of Cryptococcus neoformans var.
RT   grubii reveals complex RNA expression and microevolution leading to
RT   virulence attenuation.";
RL   PLoS Genet. 10:E1004261-E1004261(2014).
RN   [2] {ECO:0000305}
RP   DISRUPTION PHENOTYPE.
RX   PubMed=25972480; DOI=10.4049/jimmunol.1402719;
RA   Eastman A.J., He X., Qiu Y., Davis M.J., Vedula P., Lyons D.M., Park Y.D.,
RA   Hardison S.E., Malachowski A.N., Osterholzer J.J., Wormley F.L. Jr.,
RA   Williamson P.R., Olszewski M.A.;
RT   "Cryptococcal heat shock protein 70 homolog Ssa1 contributes to pulmonary
RT   expansion of Cryptococcus neoformans during the afferent phase of the
RT   immune response by promoting macrophage M2 polarization.";
RL   J. Immunol. 194:5999-6010(2015).
RN   [3] {ECO:0000305}
RP   DISRUPTION PHENOTYPE.
RX   PubMed=27866167; DOI=10.1534/genetics.116.190595;
RA   Yang D.H., Jung K.W., Bang S., Lee J.W., Song M.H., Floyd-Averette A.,
RA   Festa R.A., Ianiri G., Idnurm A., Thiele D.J., Heitman J., Bahn Y.S.;
RT   "Rewiring of Signaling Networks Modulating Thermotolerance in the Human
RT   Pathogen Cryptococcus neoformans.";
RL   Genetics 205:201-219(2017).
RN   [4] {ECO:0000305}
RP   INDUCTION.
RX   PubMed=32518190; DOI=10.1128/mbio.01127-20;
RA   Horianopoulos L.C., Hu G., Caza M., Schmitt K., Overby P., Johnson J.D.,
RA   Valerius O., Braus G.H., Kronstad J.W.;
RT   "The Novel J-Domain Protein Mrj1 Is Required for Mitochondrial Respiration
RT   and Virulence in Cryptococcus neoformans.";
RL   MBio 11:E01127-E01127(2020).
CC   -!- FUNCTION: Transcriptional coregulator that functions together with
CC       transcription factor HSF1. {ECO:0000250|UniProtKB:Q5KKP4}.
CC   -!- SUBUNIT: Interacts with transcription factor HSF1 on chromatin.
CC       {ECO:0000250|UniProtKB:Q5KKP4}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q5KKP4}.
CC   -!- INDUCTION: Induced by high temperature. {ECO:0000269|PubMed:32518190}.
CC   -!- DISRUPTION PHENOTYPE: Sensitive to high temperature (PubMed:27866167).
CC       Normal cellular melanin level (PubMed:25972480). Increases SSA2 mRNA
CC       level (PubMed:27866167). Mildly decreases virulence in a mouse
CC       intratracheal infection model (PubMed:25972480).
CC       {ECO:0000269|PubMed:25972480, ECO:0000269|PubMed:27866167}.
CC   -!- SIMILARITY: Belongs to the heat shock protein 70 family.
CC       {ECO:0000255|RuleBase:RU003322}.
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DR   EMBL; CP003830; AFR97929.1; -; Genomic_DNA.
DR   RefSeq; XP_012052712.1; XM_012197322.1.
DR   AlphaFoldDB; J9VZ70; -.
DR   SMR; J9VZ70; -.
DR   SwissPalm; J9VZ70; -.
DR   EnsemblFungi; AFR97929; AFR97929; CNAG_01727.
DR   GeneID; 23885416; -.
DR   VEuPathDB; FungiDB:CNAG_01727; -.
DR   HOGENOM; CLU_005965_3_0_1; -.
DR   Proteomes; UP000010091; Chromosome 11.
DR   GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0140662; F:ATP-dependent protein folding chaperone; IEA:InterPro.
DR   GO; GO:0003712; F:transcription coregulator activity; ISS:UniProtKB.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IC:UniProtKB.
DR   Gene3D; 1.20.1270.10; -; 1.
DR   Gene3D; 2.60.34.10; -; 1.
DR   InterPro; IPR043129; ATPase_NBD.
DR   InterPro; IPR018181; Heat_shock_70_CS.
DR   InterPro; IPR029048; HSP70_C_sf.
DR   InterPro; IPR029047; HSP70_peptide-bd_sf.
DR   InterPro; IPR013126; Hsp_70_fam.
DR   PANTHER; PTHR19375; PTHR19375; 1.
DR   Pfam; PF00012; HSP70; 1.
DR   SUPFAM; SSF100920; SSF100920; 1.
DR   SUPFAM; SSF100934; SSF100934; 1.
DR   SUPFAM; SSF53067; SSF53067; 2.
DR   PROSITE; PS00297; HSP70_1; 1.
DR   PROSITE; PS00329; HSP70_2; 1.
DR   PROSITE; PS01036; HSP70_3; 1.
PE   2: Evidence at transcript level;
KW   ATP-binding; Nucleotide-binding; Nucleus; Stress response; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..643
FT                   /note="Transcriptional coregulator SSA1"
FT                   /id="PRO_0000452018"
FT   REGION          608..643
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   643 AA;  69574 MW;  A27356CC353BFE65 CRC64;
     MVKAVGIDLG TTYSCVAVWQ NDRVEIIAND QGNRTTPSYV AFNDSERLIG DAAKNQVAMN
     PYNTVFDAKR LIGRKFEDAE VQADMKHWPF KVIDRSGKPA IQVEYRGEEK IFTPEEISSM
     VLIKMKETAE AYLGGTVSKA VVTVPAYFND SQRQATKDAG AIAGLDVLRI INEPTAAAIA
     YGLDKKSEGE KNVLIFDLGG GTFDVSLLTI EEGIFEVKAT AGDTHLGGED FDNRLVNHFV
     QEFKRKNKKD LSSNARALRR LRTACERAKR TLSSAAQTSI EIDSLFDGID FYTSITRARF
     EELCQDLFRS TMDPVEKVLR DSKIDKSSVN EIVLVGGSTR IPKIQKLVSD MFSGREPNRS
     INPDEAVAYG AAVQAAILTG DTSEATQDLL LLDVAPLSMG IETAGGIMTP LIKRNTTVPT
     KKSEVFSTYS DNQPGVLIQV FEGERAKTKD CNLLGKFDLS GIPPAPRGVP QIEVSFDVDA
     NGILNVNAAD KSTGKSNKIT ITNDKGRLSK EEIERMLAEA EKFKAEDEAA AATVQAKNGL
     ESYAYSLKTT LSDNQDKFDS ADHETLSKKV DEVISSLDTM QSASKEEFEG LQKELEAVAN
     PIMTKFYGAQ GGAPGGAPGG FPGAGGAGGA PAHEEGPSVE EVD