HSP73_DROME
ID HSP73_DROME Reviewed; 641 AA.
AC Q9BIS2; B0BNN7; P02824; Q53XF0; Q5I7H2; Q5I7H4; Q5I7H6; Q5I7H8; Q5I7I3;
AC Q5I7I6; Q5I7I8; Q9BIS1; Q9I7J6;
DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT 13-APR-2004, sequence version 2.
DT 03-AUG-2022, entry version 151.
DE RecName: Full=Major heat shock 70 kDa protein Bb;
DE Short=Heat shock protein 70Bb;
DE AltName: Full=HSP70-87C1;
GN Name=Hsp70Bb; ORFNames=CG31359;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=6777045; DOI=10.1016/0092-8674(80)90430-4;
RA Ingolia T.D., Craig E.A., McCarthy B.J.;
RT "Sequence of three copies of the gene for the major Drosophila heat shock
RT induced protein and their flanking regions.";
RL Cell 21:669-679(1980).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=3CPA126, 3CPA2, 3CPA35, 3CPA43, 3CPA47, 3CPA61, 3CPA81, 3CPA86, AUS,
RC B25, B28, FrV3-1, QD18, Z(H)1, and ZZ30;
RX PubMed=11965431; DOI=10.1007/s00239-001-0044-7;
RA Bettencourt B.R., Feder M.E.;
RT "Rapid concerted evolution via gene conversion at the Drosophila hsp70
RT genes.";
RL J. Mol. Evol. 54:569-586(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Larva, and Pupae;
RA Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.W., Champe M.,
RA Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.A.,
RA Gonzalez M., Guarin H., Kapadia B., Kronmiller B., Li P.W., Liao G.,
RA Miranda A., Mungall C.J., Nunoo J., Pacleb J.M., Paragas V., Park S.,
RA Patel S., Phouanenavong S., Wan K.H., Yu C., Lewis S.E., Rubin G.M.,
RA Celniker S.E.;
RL Submitted (JAN-2008) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-609.
RC STRAIN=IS2, IS25, IS3, IS4, IS5, IS9, Z(S)10, Z(S)11, Z(S)15, Z(S)2,
RC Z(S)24, Z(S)29, Z(S)30, Z(S)30A, Z(S)49, Z(S)53, Z(S)56, and Z(S)6;
RX PubMed=12361573; DOI=10.1016/s0960-9822(02)01181-8;
RA Maside X., Bartolome C., Charlesworth B.;
RT "S-element insertions are associated with the evolution of the Hsp70 genes
RT in Drosophila melanogaster.";
RL Curr. Biol. 12:1686-1691(2002).
RN [7]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-55.
RC STRAIN=Evolution Canyon 2000;
RX PubMed=15574805; DOI=10.1093/molbev/msi063;
RA Lerman D.N., Feder M.E.;
RT "Naturally occurring transposable elements disrupt hsp70 promoter function
RT in Drosophila melanogaster.";
RL Mol. Biol. Evol. 22:776-783(2005).
CC -!- INDUCTION: Heat shock induces the synthesis of seven proteins at five
CC otherwise inactive sites in the polytene chromosomes of fruit fly
CC larvae. Two separate sites, producing two and three copies,
CC respectively, code for the 70 kDa protein.
CC -!- MISCELLANEOUS: Most strains have three copies of the gene coding for
CC this protein at chromosome locus 87C1; two tandemly repeated Hsp70
CC genes (Hsp70Bb and Hsp70Bc) and one in reverse orientation (Hsp70Ba).
CC Some strains, including that sequenced in the Drosophila genome project
CC have three tandemly repeated Hsp70 genes (Hsp70Bb, Hsp70Bbb and
CC Hsp70Bc).
CC -!- SIMILARITY: Belongs to the heat shock protein 70 family. {ECO:0000305}.
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DR EMBL; J01104; AAD15226.1; -; Genomic_DNA.
DR EMBL; AF295951; AAG26905.1; -; Genomic_DNA.
DR EMBL; AF295952; AAG26906.1; -; Genomic_DNA.
DR EMBL; AF295953; AAG26907.1; -; Genomic_DNA.
DR EMBL; AF295954; AAG26908.1; -; Genomic_DNA.
DR EMBL; AF295955; AAG26909.1; -; Genomic_DNA.
DR EMBL; AF295956; AAG26910.1; -; Genomic_DNA.
DR EMBL; AF295957; AAG26911.1; -; Genomic_DNA.
DR EMBL; AF350476; AAK30233.1; -; Genomic_DNA.
DR EMBL; AF350477; AAK30234.1; -; Genomic_DNA.
DR EMBL; AF350478; AAK30235.1; -; Genomic_DNA.
DR EMBL; AF350479; AAK30236.1; -; Genomic_DNA.
DR EMBL; AF350480; AAK30237.1; -; Genomic_DNA.
DR EMBL; AF350481; AAK30238.1; -; Genomic_DNA.
DR EMBL; AF350482; AAK30239.1; -; Genomic_DNA.
DR EMBL; AF350483; AAK30240.1; -; Genomic_DNA.
DR EMBL; AE014297; AAN13546.1; -; Genomic_DNA.
DR EMBL; BT011058; AAR31129.1; -; mRNA.
DR EMBL; BT011379; AAR96171.1; -; mRNA.
DR EMBL; BT031349; ABY76186.1; -; mRNA.
DR EMBL; AY846848; AAW34339.1; -; Genomic_DNA.
DR EMBL; AY846849; AAW34340.1; -; Genomic_DNA.
DR EMBL; AY846850; AAW34341.1; -; Genomic_DNA.
DR EMBL; AY846851; AAW34342.1; -; Genomic_DNA.
DR EMBL; AY846852; AAW34343.1; -; Genomic_DNA.
DR EMBL; AY846853; AAW34344.1; -; Genomic_DNA.
DR EMBL; AY846854; AAW34345.1; -; Genomic_DNA.
DR EMBL; AY846855; AAW34346.1; -; Genomic_DNA.
DR EMBL; AY846856; AAW34347.1; -; Genomic_DNA.
DR EMBL; AY846857; AAW34348.1; -; Genomic_DNA.
DR EMBL; AY846858; AAW34349.1; -; Genomic_DNA.
DR EMBL; AY846859; AAW34350.1; -; Genomic_DNA.
DR EMBL; AY846860; AAW34351.1; -; Genomic_DNA.
DR EMBL; AY846861; AAW34352.1; -; Genomic_DNA.
DR EMBL; AY846862; AAW34353.1; -; Genomic_DNA.
DR EMBL; AY846863; AAW34354.1; -; Genomic_DNA.
DR EMBL; AY846864; AAW34355.1; -; Genomic_DNA.
DR EMBL; AY846865; AAW34356.1; -; Genomic_DNA.
DR EMBL; AY370940; AAQ75092.1; -; Genomic_DNA.
DR RefSeq; NP_524927.2; NM_080188.3.
DR RefSeq; NP_650209.1; NM_141952.2.
DR AlphaFoldDB; Q9BIS2; -.
DR SMR; Q9BIS2; -.
DR BioGRID; 71512; 47.
DR BioGRID; 71513; 47.
DR IntAct; Q9BIS2; 18.
DR PRIDE; Q9BIS2; -.
DR DNASU; 48583; -.
DR EnsemblMetazoa; FBtr0082637; FBpp0082106; FBgn0013278.
DR EnsemblMetazoa; FBtr0082638; FBpp0082107; FBgn0013279.
DR GeneID; 48582; -.
DR GeneID; 48583; -.
DR KEGG; dme:Dmel_CG31359; -.
DR KEGG; dme:Dmel_CG6489; -.
DR CTD; 48582; -.
DR CTD; 48583; -.
DR FlyBase; FBgn0013278; Hsp70Bb.
DR VEuPathDB; VectorBase:FBgn0013278; -.
DR VEuPathDB; VectorBase:FBgn0013279; -.
DR GeneTree; ENSGT00940000154813; -.
DR HOGENOM; CLU_005965_3_0_1; -.
DR InParanoid; Q9BIS2; -.
DR OMA; ECEACIK; -.
DR OrthoDB; 288077at2759; -.
DR PhylomeDB; Q9BIS2; -.
DR Reactome; R-DME-3371453; Regulation of HSF1-mediated heat shock response.
DR Reactome; R-DME-3371497; HSP90 chaperone cycle for steroid hormone receptors (SHR) in the presence of ligand.
DR Reactome; R-DME-3371571; HSF1-dependent transactivation.
DR Reactome; R-DME-450408; AUF1 (hnRNP D0) binds and destabilizes mRNA.
DR Reactome; R-DME-6798695; Neutrophil degranulation.
DR SignaLink; Q9BIS2; -.
DR BioGRID-ORCS; 48582; 0 hits in 3 CRISPR screens.
DR BioGRID-ORCS; 48583; 0 hits in 3 CRISPR screens.
DR PRO; PR:Q9BIS2; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0013278; Expressed in imaginal disc and 11 other tissues.
DR ExpressionAtlas; Q9BIS2; baseline and differential.
DR Genevisible; Q9BIS2; DM.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IBA:GO_Central.
DR GO; GO:0016887; F:ATP hydrolysis activity; IBA:GO_Central.
DR GO; GO:0140662; F:ATP-dependent protein folding chaperone; IEA:InterPro.
DR GO; GO:0031072; F:heat shock protein binding; IBA:GO_Central.
DR GO; GO:0051787; F:misfolded protein binding; IBA:GO_Central.
DR GO; GO:0044183; F:protein folding chaperone; IBA:GO_Central.
DR GO; GO:0051082; F:unfolded protein binding; IBA:GO_Central.
DR GO; GO:0034620; P:cellular response to unfolded protein; IBA:GO_Central.
DR GO; GO:0051085; P:chaperone cofactor-dependent protein refolding; IBA:GO_Central.
DR GO; GO:0035080; P:heat shock-mediated polytene chromosome puffing; IMP:FlyBase.
DR GO; GO:0042026; P:protein refolding; IBA:GO_Central.
DR GO; GO:0009408; P:response to heat; IMP:FlyBase.
DR GO; GO:0001666; P:response to hypoxia; IMP:FlyBase.
DR GO; GO:0006986; P:response to unfolded protein; NAS:UniProtKB.
DR GO; GO:0016192; P:vesicle-mediated transport; IBA:GO_Central.
DR Gene3D; 1.20.1270.10; -; 1.
DR Gene3D; 2.60.34.10; -; 1.
DR InterPro; IPR043129; ATPase_NBD.
DR InterPro; IPR018181; Heat_shock_70_CS.
DR InterPro; IPR029048; HSP70_C_sf.
DR InterPro; IPR029047; HSP70_peptide-bd_sf.
DR InterPro; IPR013126; Hsp_70_fam.
DR PANTHER; PTHR19375; PTHR19375; 1.
DR Pfam; PF00012; HSP70; 1.
DR SUPFAM; SSF100920; SSF100920; 1.
DR SUPFAM; SSF100934; SSF100934; 1.
DR SUPFAM; SSF53067; SSF53067; 2.
DR PROSITE; PS00297; HSP70_1; 1.
DR PROSITE; PS00329; HSP70_2; 1.
DR PROSITE; PS01036; HSP70_3; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Nucleotide-binding; Reference proteome; Stress response.
FT CHAIN 1..641
FT /note="Major heat shock 70 kDa protein Bb"
FT /id="PRO_0000078333"
FT REGION 609..641
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VARIANT 43
FT /note="D -> E (in strain: 3CPA86)"
FT VARIANT 60
FT /note="P -> S (in strain: FrV3-1)"
FT VARIANT 67
FT /note="A -> P (in Z(S)29)"
FT VARIANT 71
FT /note="I -> T (in Z(S)2)"
FT VARIANT 187
FT /note="K -> N (in strain: IS2, IS3, IS4, IS5, IS25 and
FT QD18)"
FT VARIANT 195
FT /note="F -> L (in strain: ZZ30)"
FT VARIANT 241
FT /note="E -> D (in strain: Z(H)1)"
FT VARIANT 350
FT /note="E -> D (in strain: Z(H)1)"
FT VARIANT 430
FT /note="S -> A (in strain: Z(H)1)"
FT VARIANT 437
FT /note="S -> P (in strain: IS4 and IS5)"
FT VARIANT 516
FT /note="M -> I (in strain: B28)"
FT VARIANT 524
FT /note="A -> V (in strain: B28)"
FT VARIANT 544
FT /note="V -> I (in strain: Z(S)24 and Z(S)49)"
FT VARIANT 606
FT /note="K -> T (in strain: 3CPA126)"
FT VARIANT 607
FT /note="M -> V (in strain: Z(S)30A)"
FT CONFLICT 26..28
FT /note="IAN -> NAY (in Ref. 1; AAD15226)"
FT /evidence="ECO:0000305"
FT CONFLICT 48..50
FT /note="IGD -> NGE (in Ref. 1; AAD15226)"
FT /evidence="ECO:0000305"
FT CONFLICT 306
FT /note="D -> N (in Ref. 1; AAD15226)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 641 AA; 70195 MW; F2C9C204F67EBB59 CRC64;
MPAIGIDLGT TYSCVGVYQH GKVEIIANDQ GNRTTPSYVA FTDSERLIGD PAKNQVAMNP
RNTVFDAKRL IGRKYDDPKI AEDMKHWPFK VVSDGGKPKI GVEYKGESKR FAPEEISSMV
LTKMKETAEA YLGESITDAV ITVPAYFNDS QRQATKDAGH IAGLNVLRII NEPTAAALAY
GLDKNLKGER NVLIFDLGGG TFDVSILTID EGSLFEVRST AGDTHLGGED FDNRLVTHLA
EEFKRKYKKD LRSNPRALRR LRTAAERAKR TLSSSTEATI EIDALFEGQD FYTKVSRARF
EELCADLFRN TLQPVEKALN DAKMDKGQIH DIVLVGGSTR IPKVQSLLQE FFHGKNLNLS
INPDEAVAYG AAVQAAILSG DQSGKIQDVL LVDVAPLSLG IETAGGVMTK LIERNCRIPC
KQTKTFSTYS DNQPGVSIQV YEGERAMTKD NNALGTFDLS GIPPAPRGVP QIEVTFDLDA
NGILNVSAKE MSTGKAKNIT IKNDKGRLSQ AEIDRMVNEA EKYADEDEKH RQRITSRNAL
ESYVFNVKQS VEQAPAGKLD EADKNSVLDK CNETIRWLDS NTTAEKEEFD HKMEELTRHC
SPIMTKMHQQ GAGAAGGPGA NCGQQAGGFG GYSGPTVEEV D
