HSP73_YEAST
ID HSP73_YEAST Reviewed; 649 AA.
AC P09435; D6VPS7; P18862;
DT 01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1993, sequence version 3.
DT 03-AUG-2022, entry version 201.
DE RecName: Full=Heat shock protein SSA3;
GN Name=SSA3; OrderedLocusNames=YBL075C; ORFNames=YBL06.07, YBL0610;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE.
RX PubMed=8151709; DOI=10.1007/bf00175490;
RA Boorstein W.R., Ziegelhoffer T., Craig E.A.;
RT "Molecular evolution of the HSP70 multigene family.";
RL J. Mol. Evol. 38:1-17(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=7813418; DOI=10.1002/j.1460-2075.1994.tb06923.x;
RA Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C.,
RA Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M.,
RA Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M.,
RA Cziepluch C., Demolis N., Delaveau T., Doignon F., Domdey H.,
RA Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D.,
RA Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N.,
RA Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J.,
RA Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C.,
RA Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P.,
RA Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y.,
RA Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F.,
RA Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E.,
RA Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M.,
RA Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B.,
RA Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L.,
RA Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M.,
RA Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S.,
RA Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K.,
RA Mewes H.-W., Kleine K.;
RT "Complete DNA sequence of yeast chromosome II.";
RL EMBO J. 13:5795-5809(1994).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-628.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=7992509; DOI=10.1002/yea.320100811;
RA Logghe M., Molemans F., Fiers W., Contreras R.;
RT "The two genes encoding yeast ribosomal protein S8 reside on different
RT chromosomes, and are closely linked to the hsp70 stress protein genes SSA3
RT and SSA4.";
RL Yeast 10:1093-1100(1994).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-205.
RX PubMed=1922071; DOI=10.1128/mcb.11.11.5693-5700.1991;
RA Nakazawa N., Harashima S., Oshima Y.;
RT "AAR2, a gene for splicing pre-mRNA of the MATa1 cistron in cell type
RT control of Saccharomyces cerevisiae.";
RL Mol. Cell. Biol. 11:5693-5700(1991).
RN [6]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-35.
RX PubMed=2188113; DOI=10.1128/mcb.10.6.3262-3267.1990;
RA Boorstein W.R., Craig E.A.;
RT "Transcriptional regulation of SSA3, an HSP70 gene from Saccharomyces
RT cerevisiae.";
RL Mol. Cell. Biol. 10:3262-3267(1990).
RN [7]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 602-649.
RC STRAIN=ATCC 204626 / S288c / A364A;
RX PubMed=2663194; DOI=10.1007/bf00435455;
RA Martindale D.W., Gu Z.M., Csank C.;
RT "Isolation and complete sequence of the yeast isoleucyl-tRNA synthetase
RT gene (ILS1).";
RL Curr. Genet. 15:99-106(1989).
RN [8]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
CC -!- SUBCELLULAR LOCATION: Cytoplasm.
CC -!- MISCELLANEOUS: Present with 6440 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the heat shock protein 70 family. {ECO:0000305}.
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DR EMBL; M97225; AAC37398.1; -; Unassigned_DNA.
DR EMBL; Z35836; CAA84896.1; -; Genomic_DNA.
DR EMBL; Z26879; CAA81523.1; -; Genomic_DNA.
DR EMBL; D90455; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; M36115; AAA35097.1; -; Genomic_DNA.
DR EMBL; X07886; CAA30732.1; ALT_SEQ; Genomic_DNA.
DR EMBL; BK006936; DAA07047.1; -; Genomic_DNA.
DR PIR; S36753; S36753.
DR RefSeq; NP_009478.1; NM_001178315.1.
DR AlphaFoldDB; P09435; -.
DR SMR; P09435; -.
DR BioGRID; 32627; 145.
DR DIP; DIP-2266N; -.
DR IntAct; P09435; 61.
DR MINT; P09435; -.
DR STRING; 4932.YBL075C; -.
DR iPTMnet; P09435; -.
DR UCD-2DPAGE; P09435; -.
DR MaxQB; P09435; -.
DR PaxDb; P09435; -.
DR PRIDE; P09435; -.
DR EnsemblFungi; YBL075C_mRNA; YBL075C; YBL075C.
DR GeneID; 852203; -.
DR KEGG; sce:YBL075C; -.
DR SGD; S000000171; SSA3.
DR VEuPathDB; FungiDB:YBL075C; -.
DR eggNOG; KOG0101; Eukaryota.
DR GeneTree; ENSGT00940000176322; -.
DR HOGENOM; CLU_005965_3_0_1; -.
DR InParanoid; P09435; -.
DR OMA; GCKVQNA; -.
DR BioCyc; YEAST:G3O-28967-MON; -.
DR Reactome; R-SCE-3371453; Regulation of HSF1-mediated heat shock response.
DR Reactome; R-SCE-3371497; HSP90 chaperone cycle for steroid hormone receptors (SHR) in the presence of ligand.
DR Reactome; R-SCE-3371568; Attenuation phase.
DR PRO; PR:P09435; -.
DR Proteomes; UP000002311; Chromosome II.
DR RNAct; P09435; protein.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005829; C:cytosol; IDA:SGD.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IBA:GO_Central.
DR GO; GO:0016887; F:ATP hydrolysis activity; IGI:SGD.
DR GO; GO:0140662; F:ATP-dependent protein folding chaperone; IEA:InterPro.
DR GO; GO:0031072; F:heat shock protein binding; IBA:GO_Central.
DR GO; GO:0051787; F:misfolded protein binding; IBA:GO_Central.
DR GO; GO:0044183; F:protein folding chaperone; IBA:GO_Central.
DR GO; GO:0051082; F:unfolded protein binding; IGI:SGD.
DR GO; GO:0034620; P:cellular response to unfolded protein; IBA:GO_Central.
DR GO; GO:0051085; P:chaperone cofactor-dependent protein refolding; IBA:GO_Central.
DR GO; GO:0006457; P:protein folding; IGI:SGD.
DR GO; GO:0006515; P:protein quality control for misfolded or incompletely synthesized proteins; IMP:SGD.
DR GO; GO:0042026; P:protein refolding; IBA:GO_Central.
DR GO; GO:0006616; P:SRP-dependent cotranslational protein targeting to membrane, translocation; IMP:SGD.
DR GO; GO:0016192; P:vesicle-mediated transport; IBA:GO_Central.
DR Gene3D; 1.20.1270.10; -; 1.
DR Gene3D; 2.60.34.10; -; 1.
DR InterPro; IPR043129; ATPase_NBD.
DR InterPro; IPR018181; Heat_shock_70_CS.
DR InterPro; IPR029048; HSP70_C_sf.
DR InterPro; IPR029047; HSP70_peptide-bd_sf.
DR InterPro; IPR013126; Hsp_70_fam.
DR PANTHER; PTHR19375; PTHR19375; 1.
DR Pfam; PF00012; HSP70; 1.
DR SUPFAM; SSF100920; SSF100920; 1.
DR SUPFAM; SSF100934; SSF100934; 1.
DR SUPFAM; SSF53067; SSF53067; 2.
DR PROSITE; PS00297; HSP70_1; 1.
DR PROSITE; PS00329; HSP70_2; 1.
DR PROSITE; PS01036; HSP70_3; 1.
PE 1: Evidence at protein level;
KW ATP-binding; Cytoplasm; Nucleotide-binding; Reference proteome;
KW Stress response.
FT CHAIN 1..649
FT /note="Heat shock protein SSA3"
FT /id="PRO_0000078387"
FT REGION 608..649
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 620
FT /note="G -> A (in Ref. 7; CAA30732)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 649 AA; 70547 MW; F4B136759B7D4636 CRC64;
MSRAVGIDLG TTYSCVAHFS NDRVEIIAND QGNRTTPSYV AFTDTERLIG DAAKNQAAIN
PHNTVFDAKR LIGRKFDDPE VTTDAKHFPF KVISRDGKPV VQVEYKGETK TFTPEEISSM
VLSKMKETAE NYLGTTVNDA VVTVPAYFND SQRQATKDAG TIAGMNVLRI INEPTAAAIA
YGLDKKGRAE HNVLIFDLGG GTFDVSLLSI DEGVFEVKAT AGDTHLGGED FDNRLVNHLA
TEFKRKTKKD ISNNQRSLRR LRTAAERAKR ALSSSSQTSI EIDSLFEGMD FYTSLTRARF
EELCADLFRS TLEPVEKVLK DSKLDKSQID EIVLVGGSTR IPKIQKLVSD FFNGKEPNRS
INPDEAVAYG AAVQAAILTG DQSTKTQDLL LLDVAPLSLG IETAGGIMTK LIPRNSTIPT
KKSETFSTYA DNQPGVLIQV FEGERTRTKD NNLLGKFELS GIPPAPRGVP QIDVTFDIDA
NGILNVSALE KGTGKSNKIT ITNDKGRLSK DDIDRMVSEA EKYRADDERE AERVQAKNQL
ESYAFTLKNT INEASFKEKV GEDDAKRLET ASQETIDWLD ASQAASTDEY KDRQKELEGI
ANPIMTKFYG AGAGAGPGAG ESGGFPGSMP NSGATGGGED TGPTVEEVD
