HSP75_DROME
ID HSP75_DROME Reviewed; 641 AA.
AC Q9BIR7; P02824; Q53XF0; Q9BIR8; Q9BIR9; Q9BIS0; Q9I7J6;
DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT 13-APR-2004, sequence version 2.
DT 03-AUG-2022, entry version 134.
DE RecName: Full=Major heat shock 70 kDa protein Bc;
DE Short=Heat shock protein 70Bc;
DE AltName: Full=HSP70-87C1;
GN Name=Hsp70Bc; ORFNames=CG6489;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=3CPA126, 3CPA2, 3CPA35, 3CPA43, 3CPA47, 3CPA61, 3CPA81, 3CPA86, AUS,
RC B28, FrV3-1, QD18, and Z(H)1;
RX PubMed=11965431; DOI=10.1007/s00239-001-0044-7;
RA Bettencourt B.R., Feder M.E.;
RT "Rapid concerted evolution via gene conversion at the Drosophila hsp70
RT genes.";
RL J. Mol. Evol. 54:569-586(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Larva, and Pupae;
RA Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M.,
RA Park S., Wan K.H., Yu C., Rubin G.M., Celniker S.E.;
RL Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-11.
RX PubMed=6777045; DOI=10.1016/0092-8674(80)90430-4;
RA Ingolia T.D., Craig E.A., McCarthy B.J.;
RT "Sequence of three copies of the gene for the major Drosophila heat shock
RT induced protein and their flanking regions.";
RL Cell 21:669-679(1980).
CC -!- INDUCTION: Heat shock induces the synthesis of seven proteins at five
CC otherwise inactive sites in the polytene chromosomes of fruit fly
CC larvae. Two separate sites, producing two and three copies,
CC respectively, code for the 70 kDa protein.
CC -!- MISCELLANEOUS: Most strains have three copies of the gene coding for
CC this protein at chromosome locus 87C1; two tandemly repeated Hsp70
CC genes (Hsp70Bb and Hsp70Bc) and one in reverse orientation (Hsp70Ba).
CC Some strains, including that sequenced in the Drosophila genome project
CC have three tandemly repeated Hsp70 genes (Hsp70Bb, Hsp70Bbb and
CC Hsp70Bc).
CC -!- SIMILARITY: Belongs to the heat shock protein 70 family. {ECO:0000305}.
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DR EMBL; AF295958; AAG26912.1; -; Genomic_DNA.
DR EMBL; AF295959; AAG26913.1; -; Genomic_DNA.
DR EMBL; AF295960; AAG26914.1; -; Genomic_DNA.
DR EMBL; AF295961; AAG26915.1; -; Genomic_DNA.
DR EMBL; AF295962; AAG26916.1; -; Genomic_DNA.
DR EMBL; AF350484; AAK30241.1; -; Genomic_DNA.
DR EMBL; AF350485; AAK30242.1; -; Genomic_DNA.
DR EMBL; AF350486; AAK30243.1; -; Genomic_DNA.
DR EMBL; AF350487; AAK30244.1; -; Genomic_DNA.
DR EMBL; AF350488; AAK30245.1; -; Genomic_DNA.
DR EMBL; AF350489; AAK30246.1; -; Genomic_DNA.
DR EMBL; AF350490; AAK30247.1; -; Genomic_DNA.
DR EMBL; AF350491; AAK30248.1; -; Genomic_DNA.
DR EMBL; AE014297; AAG22149.2; -; Genomic_DNA.
DR EMBL; BT011541; AAS15677.1; -; mRNA.
DR EMBL; J01104; AAD15227.1; -; Genomic_DNA.
DR PIR; A03307; HHFF72.
DR RefSeq; NP_524927.2; NM_080188.3.
DR RefSeq; NP_650209.1; NM_141952.2.
DR AlphaFoldDB; Q9BIR7; -.
DR SMR; Q9BIR7; -.
DR BioGRID; 71512; 47.
DR BioGRID; 71513; 47.
DR DNASU; 48583; -.
DR EnsemblMetazoa; FBtr0082637; FBpp0082106; FBgn0013278.
DR EnsemblMetazoa; FBtr0082638; FBpp0082107; FBgn0013279.
DR GeneID; 48582; -.
DR GeneID; 48583; -.
DR KEGG; dme:Dmel_CG31359; -.
DR KEGG; dme:Dmel_CG6489; -.
DR CTD; 48582; -.
DR CTD; 48583; -.
DR FlyBase; FBgn0013279; Hsp70Bc.
DR VEuPathDB; VectorBase:FBgn0013278; -.
DR VEuPathDB; VectorBase:FBgn0013279; -.
DR HOGENOM; CLU_005965_3_0_1; -.
DR InParanoid; Q9BIR7; -.
DR OMA; ECEACIK; -.
DR OrthoDB; 288077at2759; -.
DR PhylomeDB; Q9BIR7; -.
DR Reactome; R-DME-3371453; Regulation of HSF1-mediated heat shock response.
DR Reactome; R-DME-3371497; HSP90 chaperone cycle for steroid hormone receptors (SHR) in the presence of ligand.
DR Reactome; R-DME-3371571; HSF1-dependent transactivation.
DR Reactome; R-DME-450408; AUF1 (hnRNP D0) binds and destabilizes mRNA.
DR Reactome; R-DME-6798695; Neutrophil degranulation.
DR BioGRID-ORCS; 48582; 0 hits in 3 CRISPR screens.
DR BioGRID-ORCS; 48583; 0 hits in 3 CRISPR screens.
DR PRO; PR:Q9BIR7; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0013278; Expressed in imaginal disc and 11 other tissues.
DR ExpressionAtlas; Q9BIR7; baseline and differential.
DR Genevisible; Q9BIR7; DM.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IBA:GO_Central.
DR GO; GO:0016887; F:ATP hydrolysis activity; IBA:GO_Central.
DR GO; GO:0140662; F:ATP-dependent protein folding chaperone; IEA:InterPro.
DR GO; GO:0031072; F:heat shock protein binding; IBA:GO_Central.
DR GO; GO:0051787; F:misfolded protein binding; IBA:GO_Central.
DR GO; GO:0044183; F:protein folding chaperone; IBA:GO_Central.
DR GO; GO:0051082; F:unfolded protein binding; IBA:GO_Central.
DR GO; GO:0034620; P:cellular response to unfolded protein; IBA:GO_Central.
DR GO; GO:0051085; P:chaperone cofactor-dependent protein refolding; IBA:GO_Central.
DR GO; GO:0035080; P:heat shock-mediated polytene chromosome puffing; IMP:FlyBase.
DR GO; GO:0042026; P:protein refolding; IBA:GO_Central.
DR GO; GO:0009408; P:response to heat; IMP:FlyBase.
DR GO; GO:0001666; P:response to hypoxia; IMP:FlyBase.
DR GO; GO:0009636; P:response to toxic substance; IEP:FlyBase.
DR GO; GO:0006986; P:response to unfolded protein; NAS:UniProtKB.
DR GO; GO:0016192; P:vesicle-mediated transport; IBA:GO_Central.
DR Gene3D; 1.20.1270.10; -; 1.
DR Gene3D; 2.60.34.10; -; 1.
DR InterPro; IPR043129; ATPase_NBD.
DR InterPro; IPR018181; Heat_shock_70_CS.
DR InterPro; IPR029048; HSP70_C_sf.
DR InterPro; IPR029047; HSP70_peptide-bd_sf.
DR InterPro; IPR013126; Hsp_70_fam.
DR PANTHER; PTHR19375; PTHR19375; 1.
DR Pfam; PF00012; HSP70; 1.
DR SUPFAM; SSF100920; SSF100920; 1.
DR SUPFAM; SSF100934; SSF100934; 1.
DR SUPFAM; SSF53067; SSF53067; 2.
DR PROSITE; PS00297; HSP70_1; 1.
DR PROSITE; PS00329; HSP70_2; 1.
DR PROSITE; PS01036; HSP70_3; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Nucleotide-binding; Reference proteome; Stress response.
FT CHAIN 1..641
FT /note="Major heat shock 70 kDa protein Bc"
FT /id="PRO_0000078335"
FT REGION 609..641
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VARIANT 45
FT /note="E -> A (in strain: 3CPA81)"
FT VARIANT 74
FT /note="K -> R (in strain: 3CPA2)"
FT VARIANT 93
FT /note="S -> I (in strain: FrV3-1)"
FT VARIANT 135
FT /note="S -> G (in strain: 3CPA2)"
FT VARIANT 187
FT /note="K -> N (in strain: 3CPA2)"
FT VARIANT 210
FT /note="D -> Y (in strain: AUS)"
FT VARIANT 330
FT /note="H -> N (in strain: 3CPA43 and 3CPA81)"
FT VARIANT 342
FT /note="P -> S (in strain: 3CPA43)"
FT VARIANT 458
FT /note="D -> Y (in strain: 3CPA43 and 3CPA81)"
FT VARIANT 475
FT /note="T -> I (in strain: B28)"
FT VARIANT 601
FT /note="S -> P (in strain: 3CPA126)"
FT VARIANT 634
FT /note="G -> R (in strain: AUS)"
SQ SEQUENCE 641 AA; 70195 MW; F2C9C204F67EBB59 CRC64;
MPAIGIDLGT TYSCVGVYQH GKVEIIANDQ GNRTTPSYVA FTDSERLIGD PAKNQVAMNP
RNTVFDAKRL IGRKYDDPKI AEDMKHWPFK VVSDGGKPKI GVEYKGESKR FAPEEISSMV
LTKMKETAEA YLGESITDAV ITVPAYFNDS QRQATKDAGH IAGLNVLRII NEPTAAALAY
GLDKNLKGER NVLIFDLGGG TFDVSILTID EGSLFEVRST AGDTHLGGED FDNRLVTHLA
EEFKRKYKKD LRSNPRALRR LRTAAERAKR TLSSSTEATI EIDALFEGQD FYTKVSRARF
EELCADLFRN TLQPVEKALN DAKMDKGQIH DIVLVGGSTR IPKVQSLLQE FFHGKNLNLS
INPDEAVAYG AAVQAAILSG DQSGKIQDVL LVDVAPLSLG IETAGGVMTK LIERNCRIPC
KQTKTFSTYS DNQPGVSIQV YEGERAMTKD NNALGTFDLS GIPPAPRGVP QIEVTFDLDA
NGILNVSAKE MSTGKAKNIT IKNDKGRLSQ AEIDRMVNEA EKYADEDEKH RQRITSRNAL
ESYVFNVKQS VEQAPAGKLD EADKNSVLDK CNETIRWLDS NTTAEKEEFD HKMEELTRHC
SPIMTKMHQQ GAGAAGGPGA NCGQQAGGFG GYSGPTVEEV D
