ID   HSPC_ELECI              Reviewed;          84 AA.
AC   P83183;
DT   15-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2002, sequence version 1.
DT   25-MAY-2022, entry version 44.
DE   RecName: Full=Cysteine-rich protamine;
OS   Eledone cirrhosa (Curled octopus) (Ozaena cirrosa).
OC   Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Cephalopoda;
OC   Coleoidea; Octopodiformes; Octopoda; Incirrata; Octopodidae; Eledone.
OX   NCBI_TaxID=102876 {ECO:0000305};
RN   [1]
RP   PROTEIN SEQUENCE, FUNCTION, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RC   TISSUE=Sperm;
RX   PubMed=12113475; DOI=10.1078/0171-9335-00253;
RA   Gimenez-Bonafe P., Ribes E., Sautiere P., Gonzalez A., Kasinsky H.E.,
RA   Kouach M., Sautiere P.-E., Ausio J., Chiva M.;
RT   "Chromatin condensation, cysteine-rich protamine, and establishment of
RT   disulphide interprotamine bonds during spermiogenesis of Eledone cirrhosa
RT   (Cephalopoda).";
RL   Eur. J. Cell Biol. 81:341-349(2002).
CC   -!- FUNCTION: Protamines substitute for histones in the chromatin of sperm
CC       during the haploid phase of spermatogenesis. They compact sperm DNA
CC       into a highly condensed, stable and inactive complex. This protamine
CC       condenses spermiogenic chromatin in a pattern which comprises fibers
CC       with a progressively larger diameter and lamellae that finally undergo
CC       definitive coalescence. {ECO:0000269|PubMed:12113475}.
CC   -!- SUBUNIT: Cross-linked by interchain disulfide bonds around the DNA-
CC       helix. {ECO:0000250|UniProtKB:P02318}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:12113475}. Chromosome
CC       {ECO:0000269|PubMed:12113475}.
CC   -!- TISSUE SPECIFICITY: Testis. {ECO:0000269|PubMed:12113475, ECO:0000305}.
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DR   AlphaFoldDB; P83183; -.
DR   SMR; P83183; -.
DR   PRIDE; P83183; -.
DR   GO; GO:0000786; C:nucleosome; IEA:UniProtKB-KW.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR   GO; GO:0030261; P:chromosome condensation; IEA:UniProtKB-KW.
DR   GO; GO:0007283; P:spermatogenesis; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Chromosome; Developmental protein; Differentiation;
KW   Direct protein sequencing; Disulfide bond; DNA condensation; DNA-binding;
KW   Nucleosome core; Nucleus; Spermatogenesis.
FT   CHAIN           1..84
FT                   /note="Cysteine-rich protamine"
FT                   /id="PRO_0000106640"
FT   DISULFID        12
FT                   /note="Interchain (with C-31)"
FT                   /evidence="ECO:0000250"
FT   DISULFID        16..24
FT                   /evidence="ECO:0000250"
FT   DISULFID        31
FT                   /note="Interchain (with C-12)"
FT                   /evidence="ECO:0000250"
FT   DISULFID        63
FT                   /note="Interchain (with C-63)"
FT                   /evidence="ECO:0000250"
FT   DISULFID        64..80
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   84 AA;  10612 MW;  70932DAC3C71837E CRC64;
     FCGSKPRCRP RCKPRCRSRS KKRCRRCRRR CSRIVKKCCR RRSKCCRRRR RCPCPCPRKK
     LRCCKRRPKR RCPKRKKKRC RRKC