ID   HSSR_STAA1              Reviewed;         224 AA.
AC   A7X5Y5;
DT   29-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   23-OCT-2007, sequence version 1.
DT   25-MAY-2022, entry version 88.
DE   RecName: Full=Heme response regulator HssR;
GN   Name=hssR; OrderedLocusNames=SAHV_2345;
OS   Staphylococcus aureus (strain Mu3 / ATCC 700698).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=418127;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Mu3 / ATCC 700698;
RX   PubMed=17954695; DOI=10.1128/aac.00534-07;
RA   Neoh H.-M., Cui L., Yuzawa H., Takeuchi F., Matsuo M., Hiramatsu K.;
RT   "Mutated response regulator graR is responsible for phenotypic conversion
RT   of Staphylococcus aureus from heterogeneous vancomycin-intermediate
RT   resistance to vancomycin-intermediate resistance.";
RL   Antimicrob. Agents Chemother. 52:45-53(2008).
CC   -!- FUNCTION: Member of the two-component regulatory system HssS/HssR
CC       involved in intracellular heme homeostasis and tempering of
CC       staphylococcal virulence. Phosphorylated HssR binds to a direct repeat
CC       sequence within hrtAB promoter and activates the expression of hrtAB,
CC       an efflux pump, in response to extracellular heme, hemin, hemoglobin or
CC       blood (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- PTM: Phosphorylated by HssS. {ECO:0000250}.
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DR   EMBL; AP009324; BAF79228.1; -; Genomic_DNA.
DR   RefSeq; WP_000249497.1; NZ_CTYB01000078.1.
DR   AlphaFoldDB; A7X5Y5; -.
DR   SMR; A7X5Y5; -.
DR   KEGG; saw:SAHV_2345; -.
DR   HOGENOM; CLU_000445_30_3_9; -.
DR   OMA; KYLMSHP; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000160; P:phosphorelay signal transduction system; IEA:UniProtKB-KW.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR   CDD; cd00383; trans_reg_C; 1.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR011006; CheY-like_superfamily.
DR   InterPro; IPR001867; OmpR/PhoB-type_DNA-bd.
DR   InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR   InterPro; IPR039420; WalR-like.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   PANTHER; PTHR48111; PTHR48111; 1.
DR   Pfam; PF00072; Response_reg; 1.
DR   Pfam; PF00486; Trans_reg_C; 1.
DR   SMART; SM00448; REC; 1.
DR   SMART; SM00862; Trans_reg_C; 1.
DR   SUPFAM; SSF52172; SSF52172; 1.
DR   PROSITE; PS51755; OMPR_PHOB; 1.
DR   PROSITE; PS50110; RESPONSE_REGULATORY; 1.
PE   3: Inferred from homology;
KW   Activator; Cytoplasm; DNA-binding; Phosphoprotein; Transcription;
KW   Transcription regulation; Two-component regulatory system; Virulence.
FT   CHAIN           1..224
FT                   /note="Heme response regulator HssR"
FT                   /id="PRO_0000331325"
FT   DOMAIN          3..116
FT                   /note="Response regulatory"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT   DNA_BIND        124..222
FT                   /note="OmpR/PhoB-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01091"
FT   MOD_RES         52
FT                   /note="4-aspartylphosphate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
SQ   SEQUENCE   224 AA;  25946 MW;  251BF68572E9155A CRC64;
     MVQCLVVDDD PRILNYIASH LQTEHIDAYT QPSGEAALKL LEKQRVDIAV VDIMMDGMDG
     FQLCNTLKND YDIPVIMLTA RDALSDKERA FISGTDDYVT KPFEVKELIF RIRAVLRRYN
     INSNSEMTIG NLTLNQSYLE LQVSNKTMTL PNKEFQLLFM LAARPKQIFT REQIIEKIWG
     YDYEGDERTV DVHIKRLRQR LKKLNATLTI ETVRGQGYKV ENHV