ID   HSSR_STAA8              Reviewed;         224 AA.
AC   Q2FVQ9;
DT   29-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   21-MAR-2006, sequence version 1.
DT   25-MAY-2022, entry version 101.
DE   RecName: Full=Heme response regulator HssR;
GN   Name=hssR; OrderedLocusNames=SAOUHSC_02643;
OS   Staphylococcus aureus (strain NCTC 8325 / PS 47).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=93061;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NCTC 8325 / PS 47;
RA   Gillaspy A.F., Worrell V., Orvis J., Roe B.A., Dyer D.W., Iandolo J.J.;
RT   "The Staphylococcus aureus NCTC 8325 genome.";
RL   (In) Fischetti V., Novick R., Ferretti J., Portnoy D., Rood J. (eds.);
RL   Gram positive pathogens, 2nd edition, pp.381-412, ASM Press, Washington
RL   D.C. (2006).
CC   -!- FUNCTION: Member of the two-component regulatory system HssS/HssR
CC       involved in intracellular heme homeostasis and tempering of
CC       staphylococcal virulence. Phosphorylated HssR binds to a direct repeat
CC       sequence within hrtAB promoter and activates the expression of hrtAB,
CC       an efflux pump, in response to extracellular heme, hemin, hemoglobin or
CC       blood (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- PTM: Phosphorylated by HssS. {ECO:0000250}.
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DR   EMBL; CP000253; ABD31651.1; -; Genomic_DNA.
DR   RefSeq; WP_000249497.1; NZ_LS483365.1.
DR   RefSeq; YP_501105.1; NC_007795.1.
DR   AlphaFoldDB; Q2FVQ9; -.
DR   SMR; Q2FVQ9; -.
DR   STRING; 1280.SAXN108_2613; -.
DR   EnsemblBacteria; ABD31651; ABD31651; SAOUHSC_02643.
DR   GeneID; 3921205; -.
DR   KEGG; sao:SAOUHSC_02643; -.
DR   PATRIC; fig|93061.5.peg.2390; -.
DR   eggNOG; COG0745; Bacteria.
DR   HOGENOM; CLU_000445_30_3_9; -.
DR   OMA; KYLMSHP; -.
DR   PRO; PR:Q2FVQ9; -.
DR   Proteomes; UP000008816; Chromosome.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0032993; C:protein-DNA complex; IBA:GO_Central.
DR   GO; GO:0001216; F:DNA-binding transcription activator activity; IBA:GO_Central.
DR   GO; GO:0000156; F:phosphorelay response regulator activity; IBA:GO_Central.
DR   GO; GO:0000976; F:transcription cis-regulatory region binding; IBA:GO_Central.
DR   CDD; cd00383; trans_reg_C; 1.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR011006; CheY-like_superfamily.
DR   InterPro; IPR001867; OmpR/PhoB-type_DNA-bd.
DR   InterPro; IPR001789; Sig_transdc_resp-reg_receiver.
DR   InterPro; IPR039420; WalR-like.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   PANTHER; PTHR48111; PTHR48111; 1.
DR   Pfam; PF00072; Response_reg; 1.
DR   Pfam; PF00486; Trans_reg_C; 1.
DR   SMART; SM00448; REC; 1.
DR   SMART; SM00862; Trans_reg_C; 1.
DR   SUPFAM; SSF52172; SSF52172; 1.
DR   PROSITE; PS51755; OMPR_PHOB; 1.
DR   PROSITE; PS50110; RESPONSE_REGULATORY; 1.
PE   3: Inferred from homology;
KW   Activator; Cytoplasm; DNA-binding; Phosphoprotein; Reference proteome;
KW   Transcription; Transcription regulation; Two-component regulatory system;
KW   Virulence.
FT   CHAIN           1..224
FT                   /note="Heme response regulator HssR"
FT                   /id="PRO_0000331329"
FT   DOMAIN          3..116
FT                   /note="Response regulatory"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
FT   DNA_BIND        124..222
FT                   /note="OmpR/PhoB-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01091"
FT   MOD_RES         52
FT                   /note="4-aspartylphosphate"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00169"
SQ   SEQUENCE   224 AA;  25946 MW;  251BF68572E9155A CRC64;
     MVQCLVVDDD PRILNYIASH LQTEHIDAYT QPSGEAALKL LEKQRVDIAV VDIMMDGMDG
     FQLCNTLKND YDIPVIMLTA RDALSDKERA FISGTDDYVT KPFEVKELIF RIRAVLRRYN
     INSNSEMTIG NLTLNQSYLE LQVSNKTMTL PNKEFQLLFM LAARPKQIFT REQIIEKIWG
     YDYEGDERTV DVHIKRLRQR LKKLNATLTI ETVRGQGYKV ENHV