ID   HSTA_YEREN              Reviewed;          71 AA.
AC   P07593;
DT   01-APR-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1993, sequence version 2.
DT   25-MAY-2022, entry version 73.
DE   RecName: Full=Heat-stable enterotoxin A;
DE   AltName: Full=YST-A;
DE   Flags: Precursor;
GN   Name=ystA; Synonyms=yst;
OS   Yersinia enterocolitica.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Yersiniaceae; Yersinia.
OX   NCBI_TaxID=630;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Serotype O:8;
RX   PubMed=1427005; DOI=10.1016/0378-1097(92)90364-t;
RA   Ibrahim A., Liesack W., Pike S., Stackebrandt E.;
RT   "The polymerase chain reaction: an epidemiological tool to differentiate
RT   between two clusters of pathogenic Yersinia enterocolitica strains.";
RL   FEMS Microbiol. Lett. 76:63-66(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=W1024 / Serotype O:9;
RX   PubMed=2201642; DOI=10.1128/iai.58.9.2983-2988.1990;
RA   Delor I., Kaeckenbeeck A., Wauters G., Cornelis G.R.;
RT   "Nucleotide sequence of yst, the Yersinia enterocolitica gene encoding the
RT   heat-stable enterotoxin, and prevalence of the gene among pathogenic and
RT   nonpathogenic yersiniae.";
RL   Infect. Immun. 58:2983-2988(1990).
RN   [3]
RP   PROTEIN SEQUENCE OF 54-71.
RX   PubMed=4043080; DOI=10.1111/j.1432-1033.1985.tb09183.x;
RA   Takao T., Tominaga N., Yoshimura S., Shimonishi Y., Hara S., Inoue T.,
RA   Miyama A.;
RT   "Isolation, primary structure and synthesis of heat-stable enterotoxin
RT   produced by Yersinia enterocolitica.";
RL   Eur. J. Biochem. 152:199-206(1985).
RN   [4]
RP   TRANSCRIPTIONAL REGULATION.
RX   PubMed=7715452; DOI=10.1111/j.1365-2958.1994.tb01326.x;
RA   Mikulskis A.V., Delor I., Thi V.H., Cornelis G.R.;
RT   "Regulation of the Yersinia enterocolitica enterotoxin Yst gene. Influence
RT   of growth phase, temperature, osmolarity, pH and bacterial host factors.";
RL   Mol. Microbiol. 14:905-915(1994).
CC   -!- FUNCTION: Toxin which activates the particulate form of guanylate
CC       cyclase and increases cyclic GMP levels within the host intestinal
CC       epithelial cells.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- INDUCTION: In cultured cells, expressed only at temperatures <30
CC       degrees Celsius. Under conditions of high osmolarity and alkaline pH
CC       (as it is the case in the host's intestine), it is expressed at 37
CC       degrees Celsius. {ECO:0000269|PubMed:7715452}.
CC   -!- MISCELLANEOUS: Transcription reached a maximum at the entry to the
CC       stationary phase and significantly varied in different Y.enterocolitica
CC       strains.
CC   -!- SIMILARITY: Belongs to the heat-stable enterotoxin family.
CC       {ECO:0000305}.
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DR   EMBL; X65999; CAA46801.1; -; Genomic_DNA.
DR   EMBL; U09235; AAA18472.1; -; Unassigned_DNA.
DR   PIR; S25659; S25659.
DR   RefSeq; WP_038892553.1; NZ_UHIX01000001.1.
DR   AlphaFoldDB; P07593; -.
DR   GO; GO:0005615; C:extracellular space; IEA:InterPro.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   InterPro; IPR019806; Heat-stable_enterotox_CS.
DR   InterPro; IPR001489; Heat-stable_enterotox_STa.
DR   Pfam; PF02048; Enterotoxin_ST; 1.
DR   PROSITE; PS00273; ENTEROTOXIN_H_STABLE; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Enterotoxin; Secreted; Signal;
KW   Toxin; Virulence.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000255"
FT   PROPEP          20..53
FT                   /evidence="ECO:0000269|PubMed:4043080"
FT                   /id="PRO_0000035137"
FT   PEPTIDE         54..71
FT                   /note="Heat-stable enterotoxin A"
FT                   /id="PRO_0000035138"
FT   DISULFID        59..64
FT                   /evidence="ECO:0000250"
FT   DISULFID        60..68
FT                   /evidence="ECO:0000250"
FT   DISULFID        63..71
FT                   /evidence="ECO:0000250"
FT   VARIANT         48
FT                   /note="L -> S"
SQ   SEQUENCE   71 AA;  7494 MW;  022F99B3800C861B CRC64;
     MKKIVFVLVL MLSSFGAFGQ ETVSGQFSDA LSTPITAEVY KQACDPPLPP AEVSSDWDCC
     DVCCNPACAG C