ID   3SXNA_CALBG             Reviewed;          57 AA.
AC   P0DL82;
DT   22-NOV-2017, integrated into UniProtKB/Swiss-Prot.
DT   22-NOV-2017, sequence version 1.
DT   25-MAY-2022, entry version 9.
DE   RecName: Full=Delta-elapitoxin-Cb1a {ECO:0000303|PubMed:27763551};
DE            Short=Delta-EPTX-Cb1a {ECO:0000305|PubMed:27763551};
DE   AltName: Full=Calliotoxin {ECO:0000303|PubMed:27763551};
DE   AltName: Full=Three-finger toxin;
DE            Short=3FTx;
OS   Calliophis bivirgatus (Blue Malaysian coral snake) (Maticora bivirgata).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Lepidosauria; Squamata; Bifurcata; Unidentata; Episquamata; Toxicofera;
OC   Serpentes; Colubroidea; Elapidae; Elapinae; Calliophis.
OX   NCBI_TaxID=8633;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE, FUNCTION, SUBCELLULAR
RP   LOCATION, AND MASS SPECTROMETRY.
RC   TISSUE=Venom, and Venom gland;
RX   PubMed=27763551; DOI=10.3390/toxins8100303;
RA   Yang D.C., Deuis J.R., Dashevsky D., Dobson J., Jackson T.N., Brust A.,
RA   Xie B., Koludarov I., Debono J., Hendrikx I., Hodgson W.C., Josh P.,
RA   Nouwens A., Baillie G.J., Bruxner T.J., Alewood P.F., Lim K.K., Frank N.,
RA   Vetter I., Fry B.G.;
RT   "The snake with the scorpion's sting: novel three-finger toxin sodium
RT   channel activators from the venom of the long-glanded blue coral snake
RT   (Calliophis bivirgatus).";
RL   Toxins 8:303-323(2016).
CC   -!- FUNCTION: This toxin shifts the voltage-dependence of Nav1.4/SCN4A
CC       activation to more hyperpolarised potentials, inhibits inactivation,
CC       and produces large ramp currents, consistent with its profound effects
CC       on contractile force in an isolated skeletal muscle preparation
CC       (PubMed:27763551). This toxin produces large muscle contractions and
CC       fasciculations in the indirectly stimulated chick biventer cervicis
CC       nerve-muscle assay, which are significantly inhibited by the addition
CC       of the sodium channel antagonist tetrodotoxin (PubMed:27763551).
CC       {ECO:0000269|PubMed:27763551}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:27763551}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC       {ECO:0000305|PubMed:27763551}.
CC   -!- MASS SPECTROMETRY: Mass=6725.9; Method=Electrospray; Note=Monoisotopic
CC       mass.; Evidence={ECO:0000269|PubMed:27763551};
CC   -!- SIMILARITY: Belongs to the snake three-finger toxin family. Short-chain
CC       subfamily. {ECO:0000305}.
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DR   AlphaFoldDB; P0DL82; -.
DR   SMR; P0DL82; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0017080; F:sodium channel regulator activity; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   Gene3D; 2.10.60.10; -; 1.
DR   InterPro; IPR045860; Snake_toxin-like_sf.
DR   SUPFAM; SSF57302; SSF57302; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Ion channel impairing toxin;
KW   Neurotoxin; Secreted; Toxin; Voltage-gated sodium channel impairing toxin.
FT   CHAIN           1..57
FT                   /note="Delta-elapitoxin-Cb1a"
FT                   /evidence="ECO:0000269|PubMed:27763551"
FT                   /id="PRO_0000442419"
FT   DISULFID        3..22
FT                   /evidence="ECO:0000250|UniProtKB:P85092"
FT   DISULFID        15..36
FT                   /evidence="ECO:0000250|UniProtKB:P85092"
FT   DISULFID        40..49
FT                   /evidence="ECO:0000250|UniProtKB:P85092"
FT   DISULFID        50..55
FT                   /evidence="ECO:0000250|UniProtKB:P85092"
SQ   SEQUENCE   57 AA;  6739 MW;  069ECE4947F75018 CRC64;
     LECYDTIFKW HTMTCPEGQN LCFYYFTWRI FLVRGCTATC PVGYSHTHCC DTDKCNN