ID   HT1_IXOHO               Reviewed;          72 AA.
AC   Q5UB45;
DT   02-JUN-2021, integrated into UniProtKB/Swiss-Prot.
DT   07-DEC-2004, sequence version 1.
DT   25-MAY-2022, entry version 22.
DE   RecName: Full=Holocyclotoxin-1 {ECO:0000303|PubMed:25172536};
DE            Short=Ht-1 {ECO:0000303|PubMed:25172536};
DE   Flags: Precursor;
GN   Name=HT1 {ECO:0000312|EMBL:AAV34602.1};
OS   Ixodes holocyclus (Australian paralysis tick).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Acari;
OC   Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Ixodinae; Ixodes.
OX   NCBI_TaxID=65647;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Masina S., Thurn M.J., Padula M., Broady K.W.;
RT   "Sequence of a neurotoxin, HT-1 from the Australian paralysis tick, Ixodes
RT   holocyclus.";
RL   Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   STRUCTURE BY NMR OF 23-72, FUNCTION, SYNTHESIS OF 23-72, AND DISULFIDE
RP   BONDS.
RX   PubMed=25172536; DOI=10.1016/j.toxicon.2014.08.068;
RA   Vink S., Daly N.L., Steen N., Craik D.J., Alewood P.F.;
RT   "Holocyclotoxin-1, a cystine knot toxin from Ixodes holocyclus.";
RL   Toxicon 90:308-317(2014).
CC   -!- FUNCTION: Probable neurotoxin. {ECO:0000305|PubMed:25172536}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305|PubMed:25172536}.
CC   -!- TISSUE SPECIFICITY: Expressed by the salivary glands.
CC       {ECO:0000305|PubMed:25172536}.
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DR   EMBL; AY766147; AAV34602.1; -; mRNA.
DR   AlphaFoldDB; Q5UB45; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Disulfide bond; Neurotoxin; Secreted; Signal; Toxin.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000305|PubMed:25172536"
FT   CHAIN           23..72
FT                   /note="Holocyclotoxin-1"
FT                   /evidence="ECO:0000305|PubMed:25172536"
FT                   /id="PRO_5004262700"
FT   DISULFID        24..40
FT                   /evidence="ECO:0000269|PubMed:25172536"
FT   DISULFID        32..57
FT                   /evidence="ECO:0000269|PubMed:25172536"
FT   DISULFID        36..60
FT                   /evidence="ECO:0000269|PubMed:25172536"
FT   DISULFID        42..70
FT                   /evidence="ECO:0000269|PubMed:25172536"
SQ   SEQUENCE   72 AA;  7834 MW;  5D880DBEE051AD7D CRC64;
     MSKVTTVFIG ALVLLLLIEN GFSCTNPGKK RCNAKCSTHC DCKDGPTHNF GAGPVQCKKC
     TYQFKGEAYC KQ