HTD2_YEAST
ID HTD2_YEAST Reviewed; 280 AA.
AC P38790; D3DL16;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 1.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=Hydroxyacyl-thioester dehydratase type 2, mitochondrial;
DE EC=4.2.1.-;
DE AltName: Full=3-hydroxyacyl-[acyl-carrier-protein] dehydratase;
GN Name=HTD2; Synonyms=RMD12; OrderedLocusNames=YHR067W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8091229; DOI=10.1126/science.8091229;
RA Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z.,
RA Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T.,
RA Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P., Louis E.J.,
RA Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L.,
RA St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P.,
RA Waterston R., Wilson R., Vaudin M.;
RT "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome
RT VIII.";
RL Science 265:2077-2082(1994).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [4]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [5]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=15387819; DOI=10.1111/j.1365-2958.2004.04191.x;
RA Kastaniotis A.J., Autio K.J., Sormunen R.T., Hiltunen J.K.;
RT "Htd2p/Yhr067p is a yeast 3-hydroxyacyl-ACP dehydratase essential for
RT mitochondrial function and morphology.";
RL Mol. Microbiol. 53:1407-1421(2004).
RN [6]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], AND IDENTIFICATION BY MASS
RP SPECTROMETRY.
RX PubMed=16823961; DOI=10.1021/pr050477f;
RA Reinders J., Zahedi R.P., Pfanner N., Meisinger C., Sickmann A.;
RT "Toward the complete yeast mitochondrial proteome: multidimensional
RT separation techniques for mitochondrial proteomics.";
RL J. Proteome Res. 5:1543-1554(2006).
CC -!- FUNCTION: Mitochondrial 3-hydroxyacyl-thioester dehydratase involved in
CC fatty acid biosynthesis. Required for respiratory growth and for normal
CC mitochondrial morphology. {ECO:0000269|PubMed:15387819}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000269|PubMed:14562095,
CC ECO:0000269|PubMed:15387819, ECO:0000269|PubMed:16823961}.
CC -!- MISCELLANEOUS: Present with 799 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the HTD2 family. {ECO:0000305}.
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DR EMBL; U00061; AAB68378.1; -; Genomic_DNA.
DR EMBL; BK006934; DAA06760.1; -; Genomic_DNA.
DR PIR; S46699; S46699.
DR RefSeq; NP_011934.1; NM_001179197.1.
DR AlphaFoldDB; P38790; -.
DR SMR; P38790; -.
DR BioGRID; 36499; 469.
DR DIP; DIP-4110N; -.
DR IntAct; P38790; 2.
DR MINT; P38790; -.
DR STRING; 4932.YHR067W; -.
DR MaxQB; P38790; -.
DR PaxDb; P38790; -.
DR PRIDE; P38790; -.
DR EnsemblFungi; YHR067W_mRNA; YHR067W; YHR067W.
DR GeneID; 856464; -.
DR KEGG; sce:YHR067W; -.
DR SGD; S000001109; HTD2.
DR VEuPathDB; FungiDB:YHR067W; -.
DR eggNOG; ENOG502S5QU; Eukaryota.
DR HOGENOM; CLU_028690_2_0_1; -.
DR InParanoid; P38790; -.
DR OMA; HRIHWDL; -.
DR BioCyc; MetaCyc:G3O-31118-MON; -.
DR BioCyc; YEAST:G3O-31118-MON; -.
DR PRO; PR:P38790; -.
DR Proteomes; UP000002311; Chromosome VIII.
DR RNAct; P38790; protein.
DR GO; GO:0005739; C:mitochondrion; IDA:SGD.
DR GO; GO:0019171; F:3-hydroxyacyl-[acyl-carrier-protein] dehydratase activity; IDA:SGD.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR GO; GO:0006633; P:fatty acid biosynthetic process; IEA:InterPro.
DR GO; GO:0006631; P:fatty acid metabolic process; ISS:SGD.
DR InterPro; IPR029069; HotDog_dom_sf.
DR InterPro; IPR026223; Htd2.
DR PRINTS; PR02096; HTDHYDRTASE2.
DR SUPFAM; SSF54637; SSF54637; 1.
PE 1: Evidence at protein level;
KW Lyase; Mitochondrion; NAD; Oxidoreductase; Reference proteome.
FT CHAIN 1..280
FT /note="Hydroxyacyl-thioester dehydratase type 2,
FT mitochondrial"
FT /id="PRO_0000202898"
SQ SEQUENCE 280 AA; 33055 MW; 9FE7B9A602B7A083 CRC64;
MKSKTWIFRD VLSSHRTKAF DSLLCRRLPV SKATKHLQLG EHFLFFPPSF EKLDRDGYFN
YQNPASLLGN PDLRYRRRIW GQGELVQYLP VTLDQEYTCH ESIKYVKKIR DEHVVCIERT
LLQERPENVS SPMDICLFER RVLMYTNSPA NKTAVKMPVG EENYKILKNF TVTDMDIVAY
GQMSLNPHRI HWDKEYSRYV EGYDDIIMQG PFSVQLLQKC IQPFLEQPIR QLRYRNLNYI
YPNTTLSICQ SLSSSSGMYT FQIRDLQKAN LVYMKADVFC
