ID   HTF2_CARMO              Reviewed;          10 AA.
AC   P62542; P11385;
DT   19-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT   19-JUL-2004, sequence version 1.
DT   11-DEC-2019, entry version 42.
DE   RecName: Full=Hypertrehalosaemic factor 2;
DE   AltName: Full=Hypertrehalosaemic factor II;
DE            Short=HRTH-II;
DE            Short=HTF-II;
DE   AltName: Full=Hypertrehalosaemic neuropeptide II;
OS   Carausius morosus (Indian stick insect) (Dixippus morosus).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Polyneoptera; Phasmatodea; Verophasmatodea; Anareolatae;
OC   Lonchodidae; Lonchodinae; Carausius.
OX   NCBI_TaxID=7022;
RN   [1]
RP   PROTEIN SEQUENCE, PYROGLUTAMATE FORMATION AT GLN-1, AND MASS SPECTROMETRY.
RC   TISSUE=Corpora cardiaca;
RX   PubMed=3828078; DOI=10.1515/bchm3.1987.368.1.67;
RA   Gaede G., Rinehart K.L. Jr.;
RT   "Primary structure of the hypertrehalosaemic factor II from the corpus
RT   cardiacum of the Indian stick insect, Carausius morosus, determined by fast
RT   atom bombardment mass spectrometry.";
RL   Biol. Chem. Hoppe-Seyler 368:67-75(1987).
RN   [2]
RP   GLYCOSYLATION AT TRP-8, AND AMIDATION AT THR-10.
RC   TISSUE=Corpora cardiaca;
RX   PubMed=1482345; DOI=10.1016/0006-291x(92)90215-7;
RA   Gaede G., Kellner R., Rinehart K.L. Jr., Proefke M.L.;
RT   "A tryptophan-substituted member of the AKH/RPCH family isolated from a
RT   stick insect corpus cardiacum.";
RL   Biochem. Biophys. Res. Commun. 189:1303-1309(1992).
RN   [3]
RP   GLYCOSYLATION AT TRP-8.
RC   TISSUE=Corpora cardiaca;
RA   Gaede G.;
RT   "The adipokinetic hormone/red pigment-concentrating hormone family: new
RT   structures, unique post-translational modifications and a new physiological
RT   role.";
RL   Ann. N. Y. Acad. Sci. 839:101-104(1998).
CC   -!- FUNCTION: Hypertrehalosaemic factors are neuropeptides that elevate the
CC       level of trehalose in the hemolymph (trehalose is the major
CC       carbohydrate in the hemolymph of insects).
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- PTM: The glycosylated tryptophan appears to be identical to the C-
CC       linked mannose found in mammals, but lacks the typical W-X-X-W motif.
CC       The carbohydrate moiety is not necessary for hypertrehalosaemic
CC       activity. {ECO:0000269|PubMed:1482345, ECO:0000269|Ref.3}.
CC   -!- MASS SPECTROMETRY: Mass=1308.61; Method=FAB;
CC       Evidence={ECO:0000269|PubMed:3828078};
CC   -!- SIMILARITY: Belongs to the AKH/HRTH/RPCH family. {ECO:0000305}.
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DR   PIR; JC1416; JC1416.
DR   iPTMnet; P62542; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0005179; F:hormone activity; IEA:UniProtKB-KW.
DR   GO; GO:0007218; P:neuropeptide signaling pathway; IEA:UniProtKB-KW.
DR   InterPro; IPR002047; Adipokinetic_hormone_CS.
DR   PROSITE; PS00256; AKH; 1.
PE   1: Evidence at protein level;
KW   Amidation; Direct protein sequencing; Glycoprotein; Hormone; Neuropeptide;
KW   Pyrrolidone carboxylic acid; Secreted.
FT   PEPTIDE         1..10
FT                   /note="Hypertrehalosaemic factor 2"
FT                   /id="PRO_0000043429"
FT   MOD_RES         1
FT                   /note="Pyrrolidone carboxylic acid"
FT                   /evidence="ECO:0000269|PubMed:3828078"
FT   MOD_RES         10
FT                   /note="Threonine amide"
FT                   /evidence="ECO:0000269|PubMed:1482345"
FT   CARBOHYD        8
FT                   /note="C-linked (Man) tryptophan; atypical"
FT                   /evidence="ECO:0000269|PubMed:1482345, ECO:0000269|Ref.3"
SQ   SEQUENCE   10 AA;  1164 MW;  9B9036745771A9D1 CRC64;
     QLTFTPNWGT