HTF4_CHICK
ID HTF4_CHICK Reviewed; 657 AA.
AC P30985; Q90622; Q9PSH1;
DT 01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2000, sequence version 2.
DT 03-AUG-2022, entry version 136.
DE RecName: Full=Transcription factor 12;
DE Short=TCF-12;
DE AltName: Full=Class A helix-loop-helix transcription factor GE1;
DE AltName: Full=E-box-binding protein;
DE AltName: Full=Transcription factor HTF-4;
DE Flags: Fragment;
GN Name=TCF12; Synonyms=CTF4;
OS Gallus gallus (Chicken).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
OC Coelurosauria; Aves; Neognathae; Galloanserae; Galliformes; Phasianidae;
OC Phasianinae; Gallus.
OX NCBI_TaxID=9031;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Neural tube;
RX PubMed=8261111; DOI=10.1111/j.1460-9568.1993.tb00498.x;
RA Neuman T., Keen A., Knapik E., Shain D., Ross M., Nornes H.O., Zuber M.X.;
RT "ME1 and GE1: basic helix-loop-helix transcription factors expressed at
RT high levels in the developing nervous system and in morphogenetically
RT active regions.";
RL Eur. J. Neurosci. 5:311-318(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 153-657.
RC TISSUE=Embryonic brain;
RX PubMed=7873406; DOI=10.1016/0925-4773(94)90019-1;
RA Helms J.A., Kuratani S., Maxwell G.D.;
RT "Cloning and analysis of a new developmentally regulated member of the
RT basic helix-loop-helix family.";
RL Mech. Dev. 48:93-108(1994).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 242-657.
RX PubMed=1579475; DOI=10.1093/nar/20.7.1805;
RA Tsay H.J., Choe Y.H., Neville C.M., Schmidt J.;
RT "CTF4, a chicken transcription factor of the helix-loop-helix class A
RT family.";
RL Nucleic Acids Res. 20:1805-1805(1992).
CC -!- FUNCTION: Transcriptional regulator. Involved in the initiation of
CC neuronal differentiation. Activates transcription by binding to the E
CC box-containing promoter (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Efficient DNA binding requires dimerization with another bHLH
CC protein. Forms homo- or heterooligomers with myogenin, E12 and ITF2
CC proteins (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- DEVELOPMENTAL STAGE: Abundantly expressed during development of the
CC central nervous system, in particular in proliferating neuroblasts and
CC in cells at the initial stages of differentiation. Also expressed at
CC high levels in morphogenetically active regions such as limb buds,
CC somites and mesonephric tubules. Expression decreases once cellular
CC differentiation is over.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA49088.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=AAA85801.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; M97634; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; U12974; AAA85801.1; ALT_INIT; mRNA.
DR EMBL; X64295; CAA45573.1; ALT_FRAME; mRNA.
DR EMBL; M87337; AAA49088.1; ALT_FRAME; mRNA.
DR RefSeq; NP_990706.2; NM_205375.4.
DR AlphaFoldDB; P30985; -.
DR GeneID; 396334; -.
DR KEGG; gga:396334; -.
DR CTD; 6938; -.
DR VEuPathDB; HostDB:geneid_396334; -.
DR InParanoid; P30985; -.
DR OrthoDB; 571132at2759; -.
DR PhylomeDB; P30985; -.
DR Proteomes; UP000000539; Unplaced.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0005667; C:transcription regulator complex; IBA:GO_Central.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0070888; F:E-box binding; ISS:UniProtKB.
DR GO; GO:0046982; F:protein heterodimerization activity; ISS:UniProtKB.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0045666; P:positive regulation of neuron differentiation; ISS:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR Pfam; PF00010; HLH; 1.
DR SMART; SM00353; HLH; 1.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
PE 2: Evidence at transcript level;
KW Developmental protein; DNA-binding; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN <1..657
FT /note="Transcription factor 12"
FT /id="PRO_0000127234"
FT DOMAIN 552..605
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 1..76
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 68..89
FT /note="Leucine-zipper"
FT REGION 89..285
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 297..340
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 459..555
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 607..630
FT /note="Class A specific domain"
FT REGION 628..657
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 130..136
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255"
FT COMPBIAS 1..26
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 27..41
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 42..76
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 89..112
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 113..127
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 142..156
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 165..218
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 229..280
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 459..490
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 492..525
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 532..555
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 166
FT /note="T -> S (in Ref. 2)"
FT /evidence="ECO:0000305"
FT CONFLICT 170
FT /note="Missing (in Ref. 1)"
FT /evidence="ECO:0000305"
FT CONFLICT 174
FT /note="F -> P (in Ref. 1)"
FT /evidence="ECO:0000305"
FT CONFLICT 181
FT /note="S -> N (in Ref. 2; AAA85801)"
FT /evidence="ECO:0000305"
FT CONFLICT 189
FT /note="S -> M (in Ref. 1)"
FT /evidence="ECO:0000305"
FT CONFLICT 197
FT /note="F -> Y (in Ref. 2; AAA85801)"
FT /evidence="ECO:0000305"
FT CONFLICT 200
FT /note="I -> M (in Ref. 2; AAA85801)"
FT /evidence="ECO:0000305"
FT CONFLICT 203..205
FT /note="TST -> GSS (in Ref. 2; AAA85801)"
FT /evidence="ECO:0000305"
FT CONFLICT 211..212
FT /note="SG -> YS (in Ref. 1)"
FT /evidence="ECO:0000305"
FT CONFLICT 219
FT /note="S -> T (in Ref. 2; AAA85801)"
FT /evidence="ECO:0000305"
FT CONFLICT 230
FT /note="V -> L (in Ref. 1)"
FT /evidence="ECO:0000305"
FT CONFLICT 237
FT /note="T -> A (in Ref. 2; AAA85801)"
FT /evidence="ECO:0000305"
FT CONFLICT 247
FT /note="R -> K (in Ref. 1)"
FT /evidence="ECO:0000305"
FT CONFLICT 256
FT /note="V -> L (in Ref. 1)"
FT /evidence="ECO:0000305"
FT CONFLICT 261
FT /note="Missing (in Ref. 1)"
FT /evidence="ECO:0000305"
FT CONFLICT 264
FT /note="V -> I (in Ref. 1)"
FT /evidence="ECO:0000305"
FT CONFLICT 268..274
FT /note="DSIVGTK -> ENILGNR (in Ref. 2 and 3)"
FT /evidence="ECO:0000305"
FT CONFLICT 289
FT /note="K -> R (in Ref. 1)"
FT /evidence="ECO:0000305"
FT CONFLICT 336
FT /note="N -> S (in Ref. 1)"
FT /evidence="ECO:0000305"
FT CONFLICT 392..393
FT /note="GP -> A (in Ref. 1; M97634)"
FT /evidence="ECO:0000305"
FT CONFLICT 422..423
FT /note="SY -> NS (in Ref. 1)"
FT /evidence="ECO:0000305"
FT CONFLICT 456
FT /note="P -> S (in Ref. 1)"
FT /evidence="ECO:0000305"
FT CONFLICT 463
FT /note="S -> T (in Ref. 3; CAA45573/AAA49088)"
FT /evidence="ECO:0000305"
FT CONFLICT 474..478
FT /note="YRALS -> FR (in Ref. 1)"
FT /evidence="ECO:0000305"
FT CONFLICT 486..489
FT /note="VAIG -> GSFV (in Ref. 1)"
FT /evidence="ECO:0000305"
FT CONFLICT 638..640
FT /note="TPH -> STL (in Ref. 1)"
FT /evidence="ECO:0000305"
FT NON_TER 1
SQ SEQUENCE 657 AA; 70542 MW; 99B5C09ABC3403C8 CRC64;
MDEKGGTTSW GTSGQPSPSY DSRGFTDSPH YSDHLNDSRK GTHEGLSPTP FSNSNLIGKT
SGRGSFSLYS RDSGLSGCQS SLLRQELGLG SPAQLSSSGK PETPYYSFSA TSSRRRPKHD
SVALDPLQAK KVRKVPGLPS SVYRPSPNSD DFNRESPSYP SPKPPTSMFA STFFMQDGTH
SSSDLWSSSN GMSQPGFGGI LGTSTSHMSQ SGSYGSLHSH DRLSYPPHSV SPTDINTSLP
PMSSFHRGST SSSPYVAASH TPPVNGSDSI VGTKGNGAGG SQTGDALGKA LASIYSPDHT
SSSFPSNPST PVGSPSPLTG ASQWSRSGGQ APSSPNYENS LHSLKNRVEQ QLHEHLQDAM
SFLKDVCEQS RMEDRLDRLD DAIHVLRNHA VGPSTSLSGG HGDIHSLLGP SHNGPIGSLN
SSYGASSLVA ANRQASMVAA HREESVSLNS NHAVLPSTVS AQSTELNHKT QESYRALSGG
LQSQSVAIGP TEIKSEHKEK DENIHEPPSS DDMKSDDESS QKDIKVSSRG RTSSTNEDED
LNPEQKIERE KERRMANNAR ERLRVRDINE AFKELGRMCQ LHLKSEKPQT KLLILHQAVA
VILSLEQQVR ERNLNPKAAC LKRREEEKVS AVSAEPPTPH PGSHPGLSET TNPMGHM
