ID   HTHAR_MYCTU             Reviewed;         107 AA.
AC   O53478; L0T8F6;
DT   03-OCT-2012, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 3.
DT   25-MAY-2022, entry version 130.
DE   RecName: Full=HTH-type transcriptional regulator Rv2034;
GN   OrderedLocusNames=Rv2034;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   FUNCTION, AND DNA-BINDING.
RX   PubMed=21782791; DOI=10.1016/j.bbrc.2011.07.014;
RA   Gao C.H., Yang M., He Z.G.;
RT   "An ArsR-like transcriptional factor recognizes a conserved sequence motif
RT   and positively regulates the expression of phoP in mycobacteria.";
RL   Biochem. Biophys. Res. Commun. 411:726-731(2011).
RN   [3]
RP   FUNCTION, DNA-BINDING, ACTIVITY REGULATION, SUBUNIT, INDUCTION, AND
RP   MUTAGENESIS OF ALA-32; GLU-35 AND CYS-61.
RX   PubMed=22558408; DOI=10.1371/journal.pone.0036255;
RA   Gao C.H., Yang M., He Z.G.;
RT   "Characterization of a novel ArsR-like regulator encoded by Rv2034 in
RT   Mycobacterium tuberculosis.";
RL   PLoS ONE 7:E36255-E36255(2012).
CC   -!- FUNCTION: Involved in the regulation of lipid metabolism and hypoxic
CC       response. Positively regulates transcription of various genes, such as
CC       phoP, groEL2 and dosR. Negatively regulates its own transcription. Acts
CC       by binding to a specific palindromic sequence motif in promoter
CC       regions. {ECO:0000269|PubMed:21782791, ECO:0000269|PubMed:22558408}.
CC   -!- ACTIVITY REGULATION: DNA-binding ability is not susceptible to zinc,
CC       nickel, cobalt, cadmium, lead, copper and manganese ions.
CC       {ECO:0000269|PubMed:22558408}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000269|PubMed:22558408}.
CC   -!- INDUCTION: Negatively autoregulated. {ECO:0000269|PubMed:22558408}.
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DR   EMBL; AL123456; CCP44807.1; -; Genomic_DNA.
DR   PIR; A70943; A70943.
DR   RefSeq; NP_216550.1; NC_000962.3.
DR   RefSeq; WP_003410200.1; NZ_NVQJ01000046.1.
DR   AlphaFoldDB; O53478; -.
DR   SMR; O53478; -.
DR   STRING; 83332.Rv2034; -.
DR   PaxDb; O53478; -.
DR   DNASU; 887859; -.
DR   GeneID; 45426014; -.
DR   GeneID; 887859; -.
DR   KEGG; mtu:Rv2034; -.
DR   TubercuList; Rv2034; -.
DR   eggNOG; COG0640; Bacteria.
DR   InParanoid; O53478; -.
DR   OMA; YQQDEAW; -.
DR   PhylomeDB; O53478; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IDA:MTBBASE.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR   CDD; cd00090; HTH_ARSR; 1.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR011991; ArsR-like_HTH.
DR   InterPro; IPR001845; HTH_ArsR_DNA-bd_dom.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   Pfam; PF01022; HTH_5; 1.
DR   PRINTS; PR00778; HTHARSR.
DR   SMART; SM00418; HTH_ARSR; 1.
DR   SUPFAM; SSF46785; SSF46785; 1.
DR   PROSITE; PS50987; HTH_ARSR_2; 1.
PE   1: Evidence at protein level;
KW   Activator; DNA-binding; Reference proteome; Repressor; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..107
FT                   /note="HTH-type transcriptional regulator Rv2034"
FT                   /id="PRO_0000419177"
FT   DOMAIN          1..93
FT                   /note="HTH arsR-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00340"
FT   DNA_BIND        33..56
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00340"
FT   MUTAGEN         32
FT                   /note="A->G: Does not bind DNA, but retains the capacity to
FT                   form dimers; when associated with G-35."
FT                   /evidence="ECO:0000269|PubMed:22558408"
FT   MUTAGEN         35
FT                   /note="E->G: Does not bind DNA, but retains the capacity to
FT                   form dimers; when associated with G-32."
FT                   /evidence="ECO:0000269|PubMed:22558408"
FT   MUTAGEN         61
FT                   /note="C->A: Does not bind DNA and does not form dimers."
FT                   /evidence="ECO:0000269|PubMed:22558408"
SQ   SEQUENCE   107 AA;  11856 MW;  975FB95505F826E2 CRC64;
     MSTYRSPDRA WQALADGTRR AIVERLAHGP LAVGELARDL PVSRPAVSQH LKVLKTARLV
     CDRPAGTRRV YQLDPTGLAA LRTDLDRFWT RALTGYAQLI DSEGDDT