ID   HTIA_MANSE              Reviewed;          56 AA.
AC   P26226;
DT   01-MAY-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1992, sequence version 1.
DT   25-MAY-2022, entry version 68.
DE   RecName: Full=Hemolymph trypsin inhibitor A;
DE   AltName: Full=BPI-type;
DE   Flags: Fragment;
OS   Manduca sexta (Tobacco hawkmoth) (Tobacco hornworm).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Lepidoptera; Glossata; Ditrysia; Bombycoidea;
OC   Sphingidae; Sphinginae; Sphingini; Manduca.
OX   NCBI_TaxID=7130;
RN   [1]
RP   PROTEIN SEQUENCE.
RC   TISSUE=Larval hemolymph;
RX   PubMed=3165377; DOI=10.1016/s0021-9258(18)37989-4;
RA   Ramesh N., Sugumaran M., Mole J.E.;
RT   "Purification and characterization of two trypsin inhibitors from the
RT   hemolymph of Manduca sexta larvae.";
RL   J. Biol. Chem. 263:11523-11527(1988).
CC   -!- FUNCTION: Inhibits trypsin.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   PIR; A29235; A29235.
DR   GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR   CDD; cd00109; KU; 1.
DR   Gene3D; 4.10.410.10; -; 1.
DR   InterPro; IPR002223; Kunitz_BPTI.
DR   InterPro; IPR036880; Kunitz_BPTI_sf.
DR   Pfam; PF00014; Kunitz_BPTI; 1.
DR   SMART; SM00131; KU; 1.
DR   SUPFAM; SSF57362; SSF57362; 1.
DR   PROSITE; PS50279; BPTI_KUNITZ_2; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Protease inhibitor;
KW   Serine protease inhibitor.
FT   CHAIN           1..>56
FT                   /note="Hemolymph trypsin inhibitor A"
FT                   /id="PRO_0000155425"
FT   DOMAIN          22..>56
FT                   /note="BPTI/Kunitz inhibitor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00031"
FT   SITE            32..33
FT                   /note="Reactive bond for trypsin"
FT                   /evidence="ECO:0000250"
FT   DISULFID        22..?
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00031"
FT   DISULFID        31..55
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00031"
FT   DISULFID        47..?
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00031"
FT   UNSURE          55
FT   NON_TER         56
SQ   SEQUENCE   56 AA;  6284 MW;  9782CBE7E695D58F CRC64;
     AGLYKPPNNI ESENEVYTGN ICFLPLEVGV CRALFFRYGY DPAIKACXEF MYGGCQ