ID   HTIB_MANSE              Reviewed;          53 AA.
AC   P26227;
DT   01-MAY-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1992, sequence version 1.
DT   25-MAY-2022, entry version 71.
DE   RecName: Full=Hemolymph trypsin inhibitor B;
DE   AltName: Full=BPI-type;
DE   Flags: Fragment;
OS   Manduca sexta (Tobacco hawkmoth) (Tobacco hornworm).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Lepidoptera; Glossata; Ditrysia; Bombycoidea;
OC   Sphingidae; Sphinginae; Sphingini; Manduca.
OX   NCBI_TaxID=7130;
RN   [1]
RP   PROTEIN SEQUENCE.
RC   TISSUE=Larval hemolymph;
RX   PubMed=3165377; DOI=10.1016/s0021-9258(18)37989-4;
RA   Ramesh N., Sugumaran M., Mole J.E.;
RT   "Purification and characterization of two trypsin inhibitors from the
RT   hemolymph of Manduca sexta larvae.";
RL   J. Biol. Chem. 263:11523-11527(1988).
CC   -!- FUNCTION: Inhibits trypsin.
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DR   PIR; B29235; B29235.
DR   GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR   CDD; cd00109; KU; 1.
DR   Gene3D; 4.10.410.10; -; 1.
DR   InterPro; IPR002223; Kunitz_BPTI.
DR   InterPro; IPR036880; Kunitz_BPTI_sf.
DR   InterPro; IPR020901; Prtase_inh_Kunz-CS.
DR   Pfam; PF00014; Kunitz_BPTI; 1.
DR   PRINTS; PR00759; BASICPTASE.
DR   SMART; SM00131; KU; 1.
DR   SUPFAM; SSF57362; SSF57362; 1.
DR   PROSITE; PS00280; BPTI_KUNITZ_1; 1.
DR   PROSITE; PS50279; BPTI_KUNITZ_2; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Protease inhibitor;
KW   Serine protease inhibitor.
FT   CHAIN           1..>53
FT                   /note="Hemolymph trypsin inhibitor B"
FT                   /id="PRO_0000155426"
FT   DOMAIN          4..>53
FT                   /note="BPTI/Kunitz inhibitor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00031"
FT   SITE            14..15
FT                   /note="Reactive bond for trypsin"
FT                   /evidence="ECO:0000250"
FT   DISULFID        4..?
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00031"
FT   DISULFID        13..37
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00031"
FT   DISULFID        29..50
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00031"
FT   NON_TER         53
SQ   SEQUENCE   53 AA;  5881 MW;  3C34CFD98C6BB329 CRC64;
     EDICSLPPEV GPCRAGFLKF AYYSELNKCK LFTYGGCQGN ENNFETLQAC XQA